Aspects of a Suspended Bioprinting System Affect Cell Viability and Support Bath Properties

Navara, Adam M.; Xu, Yilan; Perez, Marissa R; Mikos, Antonios G.

doi:10.1089/ten.tea.2023.0097

Cited by 3 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the more promising bioprinting methods that aim to combine optimal biological response while simultaneously retaining good printing fidelity is suspended bioprinting [ 302 , 303 ], in which the printing process is conducted upon a sacrificial substrate bath, that can be easily removed afterwards, usually through mild heating, leaving the 3D bioprinted scaffold completely intact. This method enables the facile printing of soft hydrogels that exhibit tunable mechanical properties, without sacrificing printing resolution, giving ample space for biomechanical adaptation [ 303 , 304 ].…”

Section: Applications Of Bioprinting In Tissue and Organ Regenerationmentioning

confidence: 99%

Advances in 3D bioprinting for regenerative medicine applications

Loukelis,

Koutsomarkos,

Mikos

et al. 2024

Regenerative Biomaterials

View full text Add to dashboard Cite

Biofabrication techniques allow for the construction of biocompatible and biofunctional structures composed from biomaterials, cells and biomolecules. Bioprinting is an emerging 3D printing method which utilizes biomaterial-based mixtures with cells and other biological constituents into printable suspensions known as bioinks. Coupled with automated design protocols and based on different modes for droplet deposition, 3D bioprinters are able to fabricate hydrogel-based objects with specific architecture and geometrical properties, providing the necessary environment that promotes cell growth and directs cell differentiation towards application-related lineages. For the preparation of such bioinks, various water-soluble biomaterials have been employed, including natural and synthetic biopolymers, and inorganic materials. Bioprinted constructs are considered to be one of the most promising avenues in regenerative medicine due to their native organ biomimicry. For successful application, the bioprinted constructs should meet particular criteria such as optimal biological response, mechanical properties similar to the target tissue, high levels of reproducibility and printing fidelity, but also increased upscaling capability. In this review, we highlight the most recent advances in bioprinting, focusing on the regeneration of various tissues including bone, cartilage, cardiovascular, neural, skin, and other organs such as liver, kidney, pancreas, and lungs. We discuss the rapidly developing co-culture bioprinting systems used to resemble the complexity of tissues and organs and the crosstalk between various cell populations towards regeneration. Moreover, we report on the basic physical principles governing 3D bioprinting, and the ideal bioink properties based on the biomaterials’ regenerative potential. We examine and critically discuss the present status of 3D bioprinting regarding its applicability and current limitations that need to be overcome to establish it at the forefront of artificial organ production and transplantation.

show abstract

Section: Applications Of Bioprinting In Tissue and Organ Regenerationmentioning

confidence: 99%

Advances in 3D bioprinting for regenerative medicine applications

Loukelis,

Koutsomarkos,

Mikos

et al. 2024

Regenerative Biomaterials

View full text Add to dashboard Cite

show abstract

“…Osmotic pressure differences can drive solvent exchange between a bioink and support bath from the location with a lower concentration to that with a higher concentration. [ 40 , 83 , 84 ] In this situation, the osmotic pressure is the driving force behind the bioink diffusion, which causes a change in the diameter of bioprinted fibers. [ 40 ] In particular, diffusion limits the resolution when a bioink with extremely low viscosity is bioprinted.…”

Section: Materials Systemsmentioning

confidence: 99%

Dissecting the Interplay Mechanism among Process Parameters toward the Biofabrication of High‐Quality Shapes in Embedded Bioprinting

Wu,

Yang,

Gupta

et al. 2024

Adv Funct Materials

View full text Add to dashboard Cite

Embedded bioprinting overcomes the barriers associated with the conventional extrusion‐based bioprinting process as it enables the direct deposition of bioinks in 3D inside a support bath by providing in situ self‐support for deposited bioinks during bioprinting to prevent their collapse and deformation. Embedded bioprinting improves the shape quality of bioprinted constructs made up of soft materials and low‐viscosity bioinks, leading to a promising strategy for better anatomical mimicry of tissues or organs. Herein, the interplay mechanism among the printing process parameters toward improved shape quality is critically reviewed. The impact of material properties of the support bath and bioink, printing conditions, cross–linking mechanisms, and post‐printing treatment methods, on the printing fidelity, stability, and resolution of the structures is meticulously dissected and thoroughly discussed. Further, the potential scope and applications of this technology in the fields of bioprinting and regenerative medicine are presented. Finally, outstanding challenges and opportunities of embedded bioprinting as well as its promise for fabricating functional solid organs in the future are discussed.

show abstract

Advances in 3D printing for the repair of tympanic membrane perforation: a comprehensive review

Xue,

Chen,

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Tympanic membrane perforation (TMP) is one of the most common conditions in otolaryngology worldwide, and hearing damage caused by inadequate or prolonged healing can be distressing for patients. This article examines the rationale for utilizing three-dimensional (3D) printing to produce scaffolds for repairing TMP, compares the advantages and disadvantages of 3D printed and bioprinted grafts with traditional autologous materials and other tissue engineering materials in TMP repair, and highlights the practical and clinical significance of 3D printing in TMP repair while discussing the current progress and promising future of 3D printing and bioprinting. There is a limited number of reviews specifically dedicated to 3D printing for TMP repair. The majority of reviews offer a general overview of the applications of 3D printing in the broader realm of tissue regeneration, with some mention of TMP repair. Alternatively, they explore the biopolymers, cells, and drug molecules utilized for TMP repair. However, more in-depth analysis is needed on the strategies for selecting bio-inks that integrate biopolymers, cells, and drug molecules for tympanic membrane repair.

show abstract

Aspects of a Suspended Bioprinting System Affect Cell Viability and Support Bath Properties

Cited by 3 publications

References 40 publications

Advances in 3D bioprinting for regenerative medicine applications

Advances in 3D bioprinting for regenerative medicine applications

Dissecting the Interplay Mechanism among Process Parameters toward the Biofabrication of High‐Quality Shapes in Embedded Bioprinting

Advances in 3D printing for the repair of tympanic membrane perforation: a comprehensive review

Contact Info

Product

Resources

About