Aspect ratio of nano/microstructures determines
            <i>Staphylococcus aureus</i>
            adhesion on PET and titanium surfaces

Meinshausen, Ann-Kathrin; Herbster, Maria; Zwahr, Christoph; Soldera, Marcos; Müller, Andreas J.; Halle, Thorsten; Lasagni, Andrés Fabían; Bertrand, Jessica

doi:10.1111/jam.15033

Cited by 16 publications

(17 citation statements)

References 106 publications

(172 reference statements)

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“…This has led to the invention of many new types of titanium-based orthopedic materials with not only a higher resistance to corrosion and better tribological properties [14,15], but also improved mechanical properties [16][17][18][19]. In addition, biocompatibility [19] and resistance to bacterial infections [12,[20][21][22] can be positively influenced by those newly developed materials. As mentioned above, S. aureus and S. epidermidis are especially prone to cause device-related infections, and they are used in many biofilm-related studies in the field of orthopedics [6,[21][22][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, biocompatibility [19] and resistance to bacterial infections [12,[20][21][22] can be positively influenced by those newly developed materials. As mentioned above, S. aureus and S. epidermidis are especially prone to cause device-related infections, and they are used in many biofilm-related studies in the field of orthopedics [6,[21][22][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

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TiAl6V4 Alloy Surface Modifications and Their Impact on Biofilm Development of S. aureus and S. epidermidis

Paulitsch-Fuchs

Wolrab

Eck

et al. 2021

JFB

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One of the most serious complications following joint replacement surgeries are periprosthetic infections (PIs) arising from the adhesion of bacteria to the artificial joint. Various types of titanium–aluminum–vanadium (TiAl6V4) alloy surface modifications (coatings with silver (Ag), titanium nitride (TiN), pure titanium (cpTi), combinations of cpTi and hydroxyapatite (HA), combinations of cpTi and tricalcium phosphate (TCP), and a rough-blasted surface of TiAl6V4) have been investigated to assess their effects on biofilm development. Biofilms were grown, collected, and analyzed after 48 h to measure their protein and glucose content and the cell viability. Biofilm-associated genes were also monitored after 48 h of development. There was a distinct difference in the development of staphylococcal biofilms on the surfaces of the different types of alloy. According to the findings of this study, the base alloy TiAl6V4 and the TiN-coated surface are the most promising materials for biofilm reduction. Rough surfaces are most favorable when it comes to bacterial infections because they allow an easy attachment of pathogenic organisms. Of all rough surfaces tested, rough-blasted TiAl6V4 was the most favorable as an implantation material; all the other rough surfaces showed more distinct signs of inducing the development of biofilms which displayed higher protein and polysaccharide contents. These results are supported by RT-qPCR measurements of biofilm associated genes for Staphylococcus aureus (icaA, icaC, fnbA, fnbB, clfB, atl) and Staphylococcus epidermidis (atle, aap).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

TiAl6V4 Alloy Surface Modifications and Their Impact on Biofilm Development of S. aureus and S. epidermidis

Paulitsch-Fuchs

Wolrab

Eck

et al. 2021

JFB

View full text Add to dashboard Cite

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“…The easiest approach to translate in clinical applications would be pure surface modification without coating or the addition of antimicrobial substances. This approach was chosen by Meinshausen et al using a periodic line-like surface structure to affect bacterial adhesion [ 17 ]. Structuring titanium (Ti) surface by laser interference patterning or polyethylene terephthalate (PET) surfaces by roll-to-roll hot embossing revealed a clear effect of the aspect ratio on the adhesion of S. aureus with the most prominent effect at 0.02 to 0.05.…”

Section: Resultsmentioning

confidence: 99%

Infections @ Trauma/Orthopedic Implants: Recent Advances on Materials, Methods, and Microbes—A Mini-Review

Wildemann

2021

Materials

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Implants and materials are indispensable in trauma and orthopedic surgery. The continuous improvements of implant design have resulted in an optimized mechanical function that supports tissue healing and restoration of function. One of the still unsolved problems with using implants and materials is infection. Trauma and material implantation change the local inflammatory situation and enable bacterial survival and material colonization. The main pathogen in orthopedic infections is Staphylococcus aureus. The research efforts to optimize antimicrobial surfaces and to develop new anti-infective strategies are enormous. This mini-review focuses on the publications from 2021 with the keywords S. aureus AND (surface modification OR drug delivery) AND (orthopedics OR trauma) AND (implants OR nails OR devices). The PubMed search yielded 16 original publications and two reviews. The original papers reported the development and testing of anti-infective surfaces and materials: five studies described an implant surface modification, three developed an implant coating for local antibiotic release, the combination of both is reported in three papers, while five publications are on antibacterial materials but not metallic implants. One review is a systematic review on the prevention of stainless-steel implant-associated infections, the other addressed the possibilities of mixed oxide nanotubes. The complexity of the approaches differs and six of them showed efficacy in animal studies.

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“…However, not only do absolute values of surface feature size and density seem to be important to achieve bacteria repellent surface structures, but also their aspect ratio. Meinshausen et al generated periodic surface structures on PET foils via the DLIP technique with spatial periods larger than the size of the test strain S. aureus (diameter 1 µm, spherical shape) [34]. The authors supposed that the aspect ratio of topographical features is of special importance to accomplish bacteria repellent surfaces and reported minimal bacterial adhesion for an aspect ratio of about 0.02.…”

Section: Bacterial Attachment Depends On the Ripples Spatial Periodmentioning

confidence: 99%

“…In contrast, intrinsically bacterial-repellent surfaces can be achieved through modified surface topographies [22,[26][27][28][29], where laser-fabricated surface structures in the microand sub-micrometer range demonstrated both, increased and reduced bacterial retention [30][31][32][33][34][35][36][37]. It was suggested that the specific shape of the bacteria plays a decisive role for their adhesion as well as the depth-to-period aspect ratio of laser-generated microstructures.…”

Section: Introductionmentioning

confidence: 99%

Spatial Period of Laser-Induced Surface Nanoripples on PET Determines Escherichia coli Repellence

et al. 2021

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Bacterial adhesion and biofilm formation on surfaces are associated with persistent microbial contamination, biofouling, and the emergence of resistance, thus, calling for new strategies to impede bacterial surface colonization. Using ns-UV laser treatment (wavelength 248 nm and a pulse duration of 20 ns), laser-induced periodic surface structures (LIPSS) featuring different sub-micrometric periods ranging from ~210 to ~610 nm were processed on commercial poly(ethylene terephthalate) (PET) foils. Bacterial adhesion tests revealed that these nanorippled surfaces exhibit a repellence for E. coli that decisively depends on the spatial periods of the LIPSS with the strongest reduction (~91%) in cell adhesion observed for LIPSS periods of 214 nm. Although chemical and structural analyses indicated a moderate laser-induced surface oxidation, a significant influence on the bacterial adhesion was ruled out. Scanning electron microscopy and additional biofilm studies using a pili-deficient E. coli TG1 strain revealed the role of extracellular appendages in the bacterial repellence observed here.

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Aspect ratio of nano/microstructures determines Staphylococcus aureus adhesion on PET and titanium surfaces

Abstract: Article type : Journal of Applied Microbiology Aspect ratio of nano/microstructures determines Staphylococcus aureus adhesion on PET and titanium surfaces

Cited by 16 publications

References 106 publications

TiAl6V4 Alloy Surface Modifications and Their Impact on Biofilm Development of S. aureus and S. epidermidis

TiAl6V4 Alloy Surface Modifications and Their Impact on Biofilm Development of S. aureus and S. epidermidis

Infections @ Trauma/Orthopedic Implants: Recent Advances on Materials, Methods, and Microbes—A Mini-Review

Spatial Period of Laser-Induced Surface Nanoripples on PET Determines Escherichia coli Repellence

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