Aspartyl, arginyl and alanyl aminopeptidase activities in the hippocampus and hypothalamus of streptozotocin-induced diabetic rats

Zambotti-Villela, Leonardo; Yamasaki, Simone Cristina; Villarroel, Joyce S.; Alponti, Rafaela Fadoni; Silveira, Paulo Flávio

doi:10.1016/j.brainres.2007.07.026

Cited by 16 publications

(9 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…PGI, APB and PSA activity was fluorimetrically measured using a modified version of the method described by Zambotti-Villela et al 27. PGI was quantified with pGlu-β-naphthylamide (0.125mM) as substrate and APB with Arg-β-naphthylamide (0.125mM).…”

Section: Methodsmentioning

confidence: 99%

Altered Activity and Expression of Cytosolic Peptidases in Colorectal Cancer

et al. 2015

View full text Add to dashboard Cite

Background and Objective: The role of peptidases in carcinogenic processes and their potential usefulness as tumor markers in colorectal cancer (CRC) have been classically attributed to cell-surface enzymes. The objective of the present study was to analyze the activity and mRNA expression of three cytosolic peptidases in the CRC and to correlate the obtained results with classic histopathological parameters for tumor prognosis and survival.Methods: The activity and mRNA levels of puromycin-sensitive aminopeptidase (PSA), aminopeptidase B (APB) and pyroglutamyl-peptidase I (PGI) were measured by fluorimetric and quantitative RT-PCR methods in colorectal mucosa and tumor tissues and plasma samples from CRC patients (n=81).Results: 1) PSA and APB activity was higher in adenomas and carcinomas than in the uninvolved mucosa. 2) mRNA levels of PSA and PGI was lower in tumors. 3) PGI activity in CRC tissue correlated negatively with histological grade, tumor size and 5-year overall suvival of CRC patients. 4) Higher plasmatic APB activity was independently associated with better 5-year overall survival.Conclusions: Data suggest that cytosolic peptidases may be involved in colorectal carcinogenesis and point to the determination of this enzymes as a valuable method in the determination of CRC prognosis.

show abstract

Section: Methodsmentioning

confidence: 99%

Altered Activity and Expression of Cytosolic Peptidases in Colorectal Cancer

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Increase in circulating OXT reduces insulin secretion [78] whereas intracerebroventricular OXT causes a rise of insulin levels by activation of vagal cholinergic neurons innervating pancreatic beta-cells [79]. By contrast, insulin can alter OXT levels in the hypothalamus by activating the insulin-regulated aminopeptidase [80, 81]. Similarly, CCK interacts with OXT at many levels.…”

Section: Effects On Endocrine Systemsmentioning

confidence: 99%

Nonsocial Functions of Hypothalamic Oxytocin

et al. 2013

View full text Add to dashboard Cite

Oxytocin (OXT) is a hypothalamic neuropeptide composed of nine amino acids. The functions of OXT cover a variety of social and nonsocial activity/behaviors. Therapeutic effects of OXT on aberrant social behaviors are attracting more attention, such as social memory, attachment, sexual behavior, maternal behavior, aggression, pair bonding, and trust. The nonsocial behaviors/functions of brain OXT have also received renewed attention, which covers brain development, reproduction, sex, endocrine, immune regulation, learning and memory, pain perception, energy balance, and almost all the functions of peripheral organ systems. Coordinating with brain OXT, locally produced OXT also involves the central and peripheral actions of OXT. Disorders in OXT secretion and functions can cause a series of aberrant social behaviors, such as depression, autism, and schizophrenia as well as disturbance of nonsocial behaviors/functions, such as anorexia, obesity, lactation failure, osteoporosis, diabetes, and carcinogenesis. As more and more OXT functions are identified, it is essential to provide a general view of OXT functions in order to explore the therapeutic potentials of OXT. In this review, we will focus on roles of hypothalamic OXT on central and peripheral nonsocial functions.

show abstract

“…Mapping of APM in the hypothalamus and hippocampus of food deprived and obese rats showed the regulation of this protein under these metabolic challenges (Alponti et al 2011). APB and neutral AP in peripheral tissues and in CNS were previously reported to be altered in diabetes mellitus induced by streptozotocin (Zambotti-Villela et al 2007, Zambotti-Villela et al 2008. Although it should be predictable that changes in adipocyte subcellular distribution or regulation could occur between healthy and obese subjects, or between basal condition and insulin …”

Section: Discussionmentioning

confidence: 99%

Novel adipocyte aminopeptidases are selectively upregulated by insulin in healthy and obese rats

Alponti

Alves

Silveira

2015

Journal of Endocrinology

View full text Add to dashboard Cite

The lack of a complete assembly of the sensitivity of subcellular aminopeptidase (AP) activities to insulin in different pathophysiological conditions has hampered the complete view of the adipocyte metabolic pathways and its implications in these conditions. Here we investigated the influence of insulin on basic AP (APB), neutral puromycin-sensitive AP (PSA), and neutral puromycin-insensitive AP (APM) in high and low density microsomal and plasma membrane fractions from adipocytes of healthy and obese rats. Catalytic activities of these enzymes were fluorometrically monitoring in these fractions with or without insulin stimulus. Canonical traffic such as insulin-regulated AP was not detected for these novel adipocyte APs in healthy and obese rats. However, insulin increased APM in low density microsomal and plasma membrane fractions from healthy rats, APB in high density microsomal fraction from obese rats and PSA in plasma membrane fraction from healthy rats. A new concept of intracellular compartment-dependent upregulation of AP enzyme activities by insulin emerges from these data. This relatively selective regulation has pathophysiological significance, since these enzymes are well known to act as catalysts and receptor of peptides directly related to energy metabolism. Overall, the regulation of each one of these enzyme activities reflects certain dysfunction in obese individuals.

show abstract

Aspartyl, arginyl and alanyl aminopeptidase activities in the hippocampus and hypothalamus of streptozotocin-induced diabetic rats

Cited by 16 publications

References 37 publications

Altered Activity and Expression of Cytosolic Peptidases in Colorectal Cancer

Altered Activity and Expression of Cytosolic Peptidases in Colorectal Cancer

Nonsocial Functions of Hypothalamic Oxytocin

Novel adipocyte aminopeptidases are selectively upregulated by insulin in healthy and obese rats

Contact Info

Product

Resources

About