Aspartic Acid Stabilized Iron Oxide Nanoparticles for Biomedical Applications

Abstract

Aspartic acid stabilized iron oxide nanoparticles (A-IONPs) with globular shape and narrow size distribution were prepared by the co-precipitation method in aqueous medium. A quantum-mechanical approach to aspartic acid optimized structure displayed negative charged sites, relatively high dipole moment, and hydrophilicity, which recommended it for interaction with iron cations and surrounding water electrical dipoles. A-IONPs were characterized by TEM, XRD, ATR-FTIR, EDS, DSC, TG, DLS, NTA, and VSM techniques. Theoretical study carried out by applying Hartree-Fock and density functional algorithms suggested that some aspartic acid properties related to the interaction can develop with nanoparticles and water molecules. The results of experimental investigation showed that the mean value of particle physical diameters was 9.17 ± 2.2 nm according to TEM image analysis, the crystallite size was about 8.9 nm according to XRD data, while the magnetic diameter was about 8.8 nm, as was determined from VSM data interpretation with Langevin’s theory. The A-IONP suspension was characterized by zeta-potential of about −11.7 mV, while the NTA investigation revealed a hydrodynamic diameter of 153.9 nm. These results recommend the A-IONP suspension for biomedical applications.

Impact of Magnetite Nanoparticles Coated with Aspartic Acid on the Growth, Antioxidant Enzymes Activity and Chlorophyll Content of Maize

“…In our previous studies, magnetic nanoparticles coated with aspartic acid (A-MNP) were prepared and tested, showing great potential for biomedical applications. Therefore, starting from these results reported by Răcuciu et al (2022) [27] regarding the synthesis and characterization of A-MNP and the study on the genotoxicity of a similar A-MNP sample, reported by Răcuciu (2020) [28], we continued the research as presented in this paper dealing with the evaluation of the A-MNP influence on maize seeds. To our knowledge, no results regarding the influence of magnetic nanoparticles coated with aspartic acid on plants have been reported.…”

“…Nanoparticles' toxicity towards plants is species-dependent [55], being linked to the applied treatment (nanoparticle type and concentration) and to growth conditions. The A-MNP sample, used in this study, is suitable for biomedical applications due to dominant small particles with a narrow size distribution and a physical diameter of about 9.17 nm, showing good stability [27].…”

“…For preparing A-MNPs aqueous suspensions, a native magnetite coated with aspartic acid (C 4 H 7 NO 4 ) sample synthesized in our laboratory by an adapted controlled chemical precipitation approach at room temperature, was used. The native magnetite nanoparticles sample is suitable for biomedical applications due to its small particles, narrow size distribution, mean physical diameter of about 9.17 nm and good stability [27]. The A-MNP concentrations corresponding to the six volume fractions used in this experiment are shown in Table 1.…”

Superparamagnetic Iron Oxide Nanoparticles (SPION): From Fundamentals to State-of-the-Art Innovative Applications for Cancer Therapy

“…The list of commonly used electrostatic stabilizers includes citric acid, ammonium salts (cetyltrimethylammonium bromide (CTAB), tetramethylammonium (TMAOH)), sodium salt (sodium dodecyl sulfate (SDS)) or amino acids (L-lysine, aspartic acid [ 49 , 50 ], etc.).…”

Recent Bio-medical applications of iron oxide magnetic nanoparticles

“…Developments in the construction of magnetic nanoparticles have managed their innovative usages in cell tracking, cell separation, bio sensors, enzyme immobilization, hyperthermia treatment, immunology, drug delivery vehicles (Ferrofluids), diagnosis, etc. For these applications, the perfect IOMNPs should display a trivial size (<100 nm), splendid magnetism with negligible polydispersity, safe, biodegradable eco-friendly surface crust, and extraordinary magnetization properties [9][10][11][12][13] . IOMNPs are most popular in biomedical applications due to their low cost, less toxicity, and their unique magnetic properties.…”