Aspartic acid racemization of root dentin used for dental age estimation in a Polish population sample

Wochna, Katarzyna; Bonikowski, Radosław; Śmigielski, Janusz; Berent, Jarosław

doi:10.1007/s12024-018-9984-8

Cited by 26 publications

(29 citation statements)

References 29 publications

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“…Typically, the hydrolysis of Suc residue produces Asp and isoAsp residues at a molar ratio of approximately 1:3 [ 4 , 6 , 7 ]. These isomerized Asp residues (i.e., l -isoAsp, d -Asp, and d -isoAsp) have been detected in various aging tissues, such as eye lenses [ 1 , 8 , 9 , 10 , 11 ], brains [ 12 , 13 ], skin [ 14 ], ligaments [ 15 ], aortas [ 16 ], teeth [ 17 , 18 ], and bones [ 19 ]. The formation of isomerized Asp residues disrupts the three-dimensional structures of peptides and proteins and is considered to be associated with various age-related diseases, such as cataracts [ 1 , 8 , 9 , 10 , 11 ] and Alzheimer’s disease [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms of Succinimide Formation from Aspartic Acid Residues Catalyzed by Two Water Molecules in the Aqueous Phase

Nakayoshi

Kato

Fukuyoshi

et al. 2021

IJMS

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Aspartic acid (Asp) residues are prone to nonenzymatic isomerization via a succinimide (Suc) intermediate. The formation of isomerized Asp residues is considered to be associated with various age-related diseases, such as cataracts and Alzheimer’s disease. In the present paper, we describe the reaction pathway of Suc residue formation from Asp residues catalyzed by two water molecules using the B3LYP/6-31+G(d,p) level of theory. Single-point energies were calculated using the MP2/6-311+G(d,p) level of theory. For these calculations, we used a model compound in which an Asp residue was capped with acetyl and methylamino groups on the N- and C-termini, respectively. In the aqueous phase, Suc residue formation from an Asp residue was roughly divided into three steps, namely, iminolization, cyclization, and dehydration, with the activation energy estimated to be 109 kJ mol−1. Some optimized geometries and reaction modes in the aqueous phase were observed that differed from those in the gas phase.

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Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms of Succinimide Formation from Aspartic Acid Residues Catalyzed by Two Water Molecules in the Aqueous Phase

Nakayoshi

Kato

Fukuyoshi

et al. 2021

IJMS

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“…are based on degenerative processes of the teeth but a lot of them require extraction of the teeth and studying them which can be both expensive and unethical (12)(13)(14)(15)(16)(17). However, some changes like secondary dentin deposition in teeth happen during the entire lifespan of a subject and except for caries and specific abrasion, no other factor affects it.…”

Section: Ajdr Avicenna Journal Of Dental Researchmentioning

confidence: 99%

Age Estimation in Iranian Population Using Tooth/Pulp Ratio in Mandibular Premolars by Panoramic Radiography

Dabbaghi

Kazemi

2020

Avicenna J Dent Res

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Background: Age estimation is a fundamental part of forensic science. Considering the fact that pulp size decreases throughout the life because of secondary dentine apposition, many researchers have used it as a method for age estimation with great results. This study aimed to determine the applicability of tooth to pulp ratio in mandibular second premolars in an Iranian population using panoramic radiographs. Methods: The sample consisted of 153 digital panoramic images of male and female Iranians aged 13-70 years, and the tooth to pulp ratio of both left and right mandibular second premolars was obtained using AutoCAD software. Analysis of variance (ANOVA), Pearson’s correlation, and regression analysis were used to analyze the data. A total of 20 images were reexamined after 30 days to test intra-observer error. Results: ANOVA test showed that gender had no effect on age estimation, and there was a significant correlation between age and tooth/pulp ratio (r=0.679 for right premolar, r=0.712 for left premolars, and r=0.736 for both premolars). Linear regression formulae were created based on the results and the mean differences between the estimated and actual age were reported to be 10.35±0.51, 9.17±0.54, and 8.83±0.52 for right, left, and both premolars, respectively. Intra-observer error showed no significant difference between repeated measurements. Conclusions: Tooth/pulp ratio of mandibular second premolars is a reliable and repeatable method for age estimation in Iranian population and using premolars of both sides yields the best results.

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“…The relationship between D-Asp and age is very close and can be used as basis for age at death estimation. In forensic casework, age estimation based on the D-Asp content in dentinal protein is one of the most accurate methods for age at death estimation in adults [ 26 , 27 , 14 , 16 , 17 ]. The application of this method to archaeological samples has been less successful so far; this has been attributed to diagenetic changes [ 28 – 30 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular clocks in ancient proteins: Do they reflect the age at death even after millennia?

Mahlke

Renhart

Talaa³

et al. 2021

Int J Legal Med

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Age at death estimation in cases of human skeletal finds is an important task in forensic medicine as well as in anthropology. In forensic medicine, methods based on “molecular clocks” in dental tissues and bone play an increasing role. The question, whether these methods are applicable also in cases with post-depositional intervals far beyond the forensically relevant period, was investigated for two “protein clocks”, the accumulation of D-aspartic acid (D-Asp) and the accumulation of pentosidine (Pen) in dentine. Eight teeth of skeletons from different burial sites in Austria and with post-depositional intervals between c. 1216 and c. 8775 years were analysed. The results of age at death estimation based on D-Asp and Pen in dentine were compared to that derived from a classical morphological examination. Age at death estimation based on D-Asp resulted consistently in false high values. This finding can be explained by a post-mortem accumulation of D-Asp that may be enhanced by protein degradation. In contrast, the Pen-based age estimates fitted well with the morphological age diagnoses. The described effect of post-mortem protein degradation is negligible in forensically relevant time horizons, but not for post-depositional intervals of thousands of years. That means that the “D-Asp clock” loses its functionality with increasing post-depositional intervals, whereas Pen seems to be very stable. The “Pen-clock” may have the potential to become an interesting supplement to the existing repertoire of methods even in cases with extremely long post-depositional intervals. Further investigations have to test this hypothesis.

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Aspartic acid racemization of root dentin used for dental age estimation in a Polish population sample

Cited by 26 publications

References 29 publications

Molecular Mechanisms of Succinimide Formation from Aspartic Acid Residues Catalyzed by Two Water Molecules in the Aqueous Phase

Molecular Mechanisms of Succinimide Formation from Aspartic Acid Residues Catalyzed by Two Water Molecules in the Aqueous Phase

Age Estimation in Iranian Population Using Tooth/Pulp Ratio in Mandibular Premolars by Panoramic Radiography

Molecular clocks in ancient proteins: Do they reflect the age at death even after millennia?

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