Aspartate aminotransferase in ejaculates of manchego and merino rams after minimal and maximal sperm damage

Borque, C.; Ayllón, A.

doi:10.1016/s0093-691x(96)00266-x

Cited by 7 publications

(6 citation statements)

References 16 publications

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“…One unit of enzyme was considered to be the amount producing an optical density change of 2.07/min at 340 nm, which corresponded to oxidation of 1 μmol nicotinamide adenine dinucleotide (NADH) under the conditions of the assay. The LDH activity was measured using the method of Borque & Ayllón (1996). One unit of GOT activity was equivalent to a change in optical density of 0.001/min at 340 nm.…”

Section: Methodsmentioning

confidence: 99%

Effect of alpha-lipoic acid on boar spermatozoa quality during freezing–thawing

Shen

Jiang

et al. 2015

Zygote

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Alpha-lipoic acid (ALA) is known to be a natural antioxidant. The aim of the present study was to evaluate the cryoprotective effect of ALA on the motility of boar spermatozoa and its antioxidant effect on boar spermatozoa during freezing-thawing. Different concentrations (2.0, 4.0, 6.0, 8.0 or 10.0 mg/ml) of ALA were added to the extender used to freeze boar semen, and the effects on the quality and endogenous antioxidant enzyme activities of frozen-thawed spermatozoa were assessed. The results indicated that the addition of ALA to the extender resulted in a higher percentage of motile spermatozoa post-thaw (P < 0.05). The activities of superoxide dismutase, lactate dehydrogenase, glutamic-oxaloacetic transaminase and catalase improved after adding ALA to the extender (P < 0.05). Artificial insemination results showed that pregnancy rate and litter size were significantly higher at 6.0 mg/ml in the ALA group than in the control group (P < 0.05). In conclusion, ALA conferred a cryoprotective capacity to the extender used for boar semen during the process of freezing-thawing, and the optimal concentration of ALA for the frozen extender was 6.0 mg/ml.

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Section: Methodsmentioning

confidence: 99%

Effect of alpha-lipoic acid on boar spermatozoa quality during freezing–thawing

Shen

Jiang

et al. 2015

Zygote

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“…GOT Assay. The GOT activity was measured at 25°C using a method based on the fact that GOT catalyzed the transfer of the l-aspartate amino group to oxoglutarate, observed with the spectrophotometer (Shanghai Spectrophotometer Co. Ltd.) at 340 nm (Borque and Ayllón, 1996). One unit of GOT activity was equivalent to a change in optical density of 0.001/ min at 340 nm.…”

Section: Biochemical Assaymentioning

confidence: 99%

Testing Rhodiola sachalinensis saccharide as cryoprotectant for bovine spermatozoa

Cao

Sun

et al. 2013

Journal of Dairy Science

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Rhodiola sachalinensis saccharide (RSS) was extracted from the rhizome of Herba Rhodiolae and was expected as a novel cryoprotectant. The aim of this study was to test the effects of RSS on motility of bull sperm and the activities of superoxide dismutase (SOD), lactate dehydrogenase (LDH), and glutamic oxaloacetic transaminase (GOT) in bull sperm during cryopreservation. Rhodiola sachalinensis saccharide was added at the concentrations of 0.02, 0.04, 0.06, 0.08, and 0.10 mg/mL to the extenders, which were used to store bovine semen. It was found that the RSS-added extends resulted in a higher percentage of cryopreserved sperm motility, mitochondrial activity, and membrane and acrosome integrity than those of RSS-free extenders. The SOD, LDH, and GOT activities were all decreased during the process of freezing and thawing. The extenders supplemented with RSS improved the SOD, LDH, and GOT activities after cryopreservation compared with the RSS-free groups. In conclusion, RSS conferred great cryoprotective capacity to the basic extender for bull spermatozoa during the process of freezing-thawing, and the optimal concentration of RSS for the extender was 0.06 mg/mL.

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“…Neste estudo, o estresse causado pelo resfriamento não foi suficiente para promover diferenças nos níveis de AAT liberados. Borque & Ayllón (1996), trabalhando com sêmen de carneiros, submeteram as células espermáticas ao que foi chamado de estresse mínimo (centrifugação da amostra de sêmen fresco) e estresse máximo (congelamento e descongelamento sucessivos), e encontraram um efeito significativo (P<0,01) na atividade enzimática da AAT depois que os ejaculados foram submetidos ao processo de estresse máximo. No entanto, num estudo conduzido por Ciereszko et al (1992), para avaliar e caracterizar a atividade da AAT no sêmen de reprodutor suíno e determinar o efeito na taxa de diluição foi encontrado que a atividade da AAT que tinha saído do espermatozóide aumentava gradualmente durante o período de estoque em vários diluentes a uma temperatura de 16-18° C. Todos estes achados sugerem que para o sêmen suíno resfriado a validade da técnica de avaliação do perfil enzimático é controversa.…”

Section: Resultsunclassified

“…Porém, o resultado da fertilidade do animal requer um considerável número de animais, tempo e recursos financeiros. Além disso, as diferenças peculiares entre as fêmeas podem confundir a interpretação dos resultados (BORQUE & AYLLÓN, 1996).…”

Section: Introductionunclassified

Efeito da adição de cloreto de cálcio sobre a qualidade espermática e atividade da aspartato amino transferase no sêmen resfriado de suíno

et al. 2007

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RESUMOO experimento foi realizado com o objetivo de testar dois processos de resfriamento de sêmen suíno, analisar o efeito da adição de CaCl 2 ao diluidor BTS e testar o método de avaliação do perfil enzimático da Aspartato Aminotransferase (AAT) sobre a qualidade espermática. Foram utilizados 12 ejaculados suínos de animais procedentes do setor de Suinocultura DZO/UFLA. Estes ejaculados foram diluídos e receberam diferentes concentrações de CaCl 2 (A: 0,0; B: 2,5; C: 5,0 e D: 7,5 mM). As amostras dos ejaculados foram submetidas a três processos de resfriamento (1: convencional 15° C ; 2: lento 15° C/5° C; 3: rápido 5° C), sendo que cada ejaculado ficou armazenado por um período de 72 horas para avaliações da qualidade espermática, constituindo os tratamentos experimentais. Os parâmetros seminais avaliados foram motilidade e vigor espermáticos e perfil enzimático da AAT. Houve diferença significativa (P<0,05) quanto aos níveis de CaCl 2 para o parâmetro de motilidade espermática, sendo que níveis maiores deste sal apresentaram resultados mais favoráveis à motilidade espermática. Para as variáveis vigor espermático e para o perfil da AAT não foi observado efeito da adição deste sal. Quanto ao processo de resfriamento, foi observada diferença significativa (P<0,05) para os parâmetros de motilidade e vigor espermáticos. Para o perfil enzimático não foi observado diferença significativa (P>0,05). Concluise que a adição de CaCl 2 melhora a motilidade espermática das amostras dos ejaculados suínos e que o processo de resfriamento lento substitui o processo convencional sem afetar a qualidade espermática do sêmen submetido à refrigeração. A avaliação da AAT não é válida para sêmen resfriado.Termos para indexação: Sêmen resfriado, suínos, parâmetros espermáticos. ABSTRACTThe study was carried out with objective to test two swine semen cooling processes and verify the effects of adding chloride of calcium (CaCl 2 ) on semen dilutor BTS and also to test the evaluation method of Aspartate Aminotransferase (AAT) enzymatic profile on the cooled swine semen spermatic quality. Were used twelve samples of ejaculation of breeders supplied by the Swine Breeding section at the DZO/UFLA. The samples were diluted and received different concentrations of CaCl 2 (A: 0.0; B: 2.5; C 5.0; D 7.5mM). The samples of ejaculation were submitted to three processes of cooling: 1 standard cooling (15° C); 2 slow cooling (15° C/ 5° C); 3 fast cooling (5° C), and each sample of ejaculation was stored for a period of 72 hours to evaluation of spermatic quality, over all experimental treatments. The seminal parameters evaluated were the spermatic motility and strength and enzymatic profile of the AAT. A meaningful difference was verified (P< 0.05) in the levels of CaCl 2 for the parameters of spermatic motility, high levels of this salt showed better results in this parameter . The variables spermatic strength and AAT profile, shown no effects for any addition of CaCl 2 . For the cooling process, a meaningful difference was verified (P<0.05) in the...

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Aspartate aminotransferase in ejaculates of manchego and merino rams after minimal and maximal sperm damage

Cited by 7 publications

References 16 publications

Effect of alpha-lipoic acid on boar spermatozoa quality during freezing–thawing

Effect of alpha-lipoic acid on boar spermatozoa quality during freezing–thawing

Testing Rhodiola sachalinensis saccharide as cryoprotectant for bovine spermatozoa

Efeito da adição de cloreto de cálcio sobre a qualidade espermática e atividade da aspartato amino transferase no sêmen resfriado de suíno

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