Aspartate aminotransferase—immunoglobulin G complexes in sera of two patients with acute hepatitis and immunoblatic lymphadenopathy

Kanemitsu, Fusae

doi:10.1016/0009-8981(86)90021-5

Cited by 14 publications

(11 citation statements)

References 2 publications

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“…Binding of serum enzyme to serum immunoglobulin is well known in amylase, LDH, and Al-P [1][2][3][4][5][6] . Because AST is also complexed with immunoglobulins such as IgG and IgA [7][8][9][10][11][12] , markedly high values of serum AST are sometimes observed whether liver disease exists or not.…”

Section: Discussionmentioning

confidence: 99%

“…Because the serum activities of the enzymes are higher in patients with enzyme-immunoglobulin complexes, which are frequently observed in patients with autoimmune diseases, it is important to evaluate enzymeimmunoglobulin complexes in clinical practice. Some investigators [7][8][9][10][11][12][13][14] have reported that AST-immunoglobulin complexes are observed in several diseases, although the incidence is low. The ratio of AST to alanine aminotransferase (AST/ALT) increases with the progression of chronic liver diseases [15] .…”

Section: Introductionmentioning

confidence: 99%

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Aspartate aminotransferase-immunoglobulin complexes in patients with chronic liver disease

Tameda

Shiraki²,

Ooi³

et al. 2005

WJG

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AIM:To determine the complex of AST and immunoglobulin and to investigate its clinical significance in patients with liver disease. METHODS:The complex of AST and immunoglobulin was determined by encounter immunoelectrophoresis and its clinical significance was investigated in 128 patients with liver disease. RESULTS:AST was bound to immunoglobulin of antiimmunoglobulin A (IgA) class, but any binding to antiimmunoglobulin G and anti-immunoglobulin M classes was not observed. Although the incidence of ASTimmunoglobulin complex was 41.8% in chronic hepatitis (CH), the incidences in liver cirrhosis and hepatocellular carcinoma were 62.2 and 90.0%, respectively. In alcoholic liver disease with high level of serum IgA, the incidence of the complex was 66.7%, which was higher than that in CH. The ratio of binding to lambda-chain of IgA was higher than that to kappa-chain of IgA. The serum level of IgA and the ratio of AST/alanine aminotransferase (ALT) were significantly higher in patients with AST-IgA complex than in those without complex. CONCLUSION:These results suggest that AST-IgA complex in patients with progressive liver diseases and alcoholic liver injury can lead to elevation of the ratio of AST/ALT.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Aspartate aminotransferase-immunoglobulin complexes in patients with chronic liver disease

Tameda

Shiraki²,

Ooi³

et al. 2005

WJG

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“…Ugyanakkor a makro-AST megjelenhet betegség fenállásakor is. Jelenlétét leírták krónikus májbetegségekben, monoklonális gammopathiában, májáttéttel járó tüdőrákban, szepszis után, allergiás rhinitis specifi kus allergénnel történő injekciós terápia után, colitis ulcerosában, akut hepatitisben és immunoblastos lymphadenopathiában [17,18,19,20,21,22,23,24,25]. Kimutatására többféle módszert is kidolgoztak: hűtőteszt, PEG-precipitációs teszt, méretki-zárásos kromatográfi a, elektroforézis és piridoxál-5-foszfát-aktivációs módszer [11,14,16].…”

Section: Megbeszélésunclassified

Isolated aspartate aminotransferase elevation in a young, healthy person. Case report

Kovács

Varga

Szabó³

et al. 2014

Orvosi Hetilap

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A szerzők egy fi atal, egészséges egyén esetét ismertetik, akinél az izolált aszpartát-aminotranszferáz-emelkedés diagnosztikai nehézséget jelentett. A probléma megoldására polietilénglikol precipitációs tesztet, aszpartát-aminotranszferáz-szérumelektroforézist és immunfi xációt alkalmaztak. Kimutatták, hogy a beteg szérummintáiban polietilén glikol-precipitációt követően az aszpartát-aminotranszferáz-aktivitás majdnem teljesen megszűnt. Az aszpartát-aminotranszferáz a kontrollmintákban az anód, míg a betegmintában a katód felé vándorolt. Immunfi xációs gélen szélesebb és intenzívebben festődő csík volt látható az immunglobulin-A-régióban a betegmintában a kontrollmintához képest. Ezek a laboratóriumi tesztek megerősítették, hogy az aszpartát-aminotranszferáz-aktivitás makroformáció következményeként is megemelkedhet. Esetükben az emelkedett immunglobulin-A-szint és a szelektív poliklonális immunglobulin-A-(κ-és λ-könnyűláncok) növekedés makroformátumot jelzett, amit az aszpartát-aminotranszferázhoz való immunglobulin-A-kötődés hozott létre. Orv. Hetil., 2014, 155(39), 1558-1562. Kulcsszavak: makroenzimek, makroaszpartát-aminotranszferáz, májbetegségIsolated aspartate aminotransferase elevation in a young, healthy person. Case reportThe authors present diagnostic methods used in a young healthy person who had isolated aspartate aminotransferase elevation. Polyethylene glycol precipitation test, aspartate aminotransferase serum electrophoresis and immunofi xation were performed for measuring the macro-aspartate aminotransferase. It was found that aspartate aminotransferase activity in the patient was almost completely eliminated after precipitation of immunoglobulins with polyethylene glycol. In addtion, aspartate aminotransferase migrated in the control samples to the anode while in the patient towards the cathode. Finally, a wider and more intense staining band was visible in the region of immunoglobulin A in the patient sample on the immunofi xation gel as compared to the control sample. The authors conclude that that increased aspartate aminotransferase activity was due to macro formation. The elevated level of immunoglobulin A and selective increase of polyclonal immunoglobulin A (κ and λ light chains) indicated that the macro format was created by immunoglobulin A bound to aspartate aminotransferase.

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“…The macro aspartate aminotransferase migrated as a broad band between the cytoplasmic aspartate aminotransferase and mitochondrial aspartate aminotransferase iso-enzymes in cellulose acetate or agarose electrophoresis (44, 46, 48 -51). IgG K was demonstrated in 2 cases (45,49) and IgG K + IgG x in 3 other cases (46,49). IgG K was demonstrated in 2 cases (45,49) and IgG K + IgG x in 3 other cases (46,49).…”

Section: Aspartate and Alanine Aminotransferasesmentioning

confidence: 99%

“…Based on the virtual absence of the cytoplasmic iso-enzyme in electrophoresis, this iso-enzyme is presumed to be involved in at least 8 patients (44,46,47,50). In one patient, the IgG fraction specifically bound the cytoplasmic iso-enzyme, whereas the IgA component complexed both the cytoplasmic and mitochondrial iso-enzyme (49). In one patient, the IgG fraction specifically bound the cytoplasmic iso-enzyme, whereas the IgA component complexed both the cytoplasmic and mitochondrial iso-enzyme (49).…”

Section: Aspartate and Alanine Aminotransferasesmentioning

confidence: 99%

Review

1990

Clinical Chemistry and Laboratory Medicine

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The presence of a macro enzyme, i. e. an enzyme with an abnormally high molecular weight, frequently leads to a genuinely increased serum activity or an interference in laboratory assays. This may thereby result in false positive diagnoses, such as acute myocardial infarction or pancreatitis. The various forms of macro enzymes are reviewed. In the case of immune complexes the immunoglobulins and (iso)enzymes involved are discussed, as well as the specificity of the interaction, the prevalence and clinical relevance, associations with autoimmune or other diseases, and their behaviour in laboratory assays.

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Aspartate aminotransferase—immunoglobulin G complexes in sera of two patients with acute hepatitis and immunoblatic lymphadenopathy

Cited by 14 publications

References 2 publications

Aspartate aminotransferase-immunoglobulin complexes in patients with chronic liver disease

Aspartate aminotransferase-immunoglobulin complexes in patients with chronic liver disease

Isolated aspartate aminotransferase elevation in a young, healthy person. Case report

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