Aspartame-fed zebrafish exhibit acute deaths with swimming defects and saccharin-fed zebrafish have elevation of cholesteryl ester transfer protein activity in hypercholesterolemia

Kim, Jae-Yong; Seo, Juyi; Cho, Kyung‐Hyun

doi:10.1016/j.fct.2011.08.001

Cited by 61 publications

(51 citation statements)

References 37 publications

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“…This result suggests that modification of HDL 3 by saccharin and acesulfame K did not impair the CETP activity, although the antioxidant activity was much impaired. This result is in good agreement with our previous report, which found that saccharinfed zebrafish show the highest serum CETP activity [4]. It is interesting that all AS-treated HDL 3 showed increased CETP activity with loss of PON activity.…”

Section: Cetp Activity Is Enhanced By As Treatmentsupporting

confidence: 93%

“…In addition, saccharin-fed zebrafish show an increased atherogenic serum lipid profile along with elevated CETP activity [4]. Taken together, these findings suggest that AS has putative toxicity in the human cardiovascular system zebrafish for 2 weeks showed remarkable increase of serum cholesterol and TG, suggesting that increase of cardiovascular risk.…”

Section: Introductionmentioning

confidence: 95%

“…Among the various AS, saccharin induced the most severe impairment of apoA-I in addition to pro-senescence activity [3]. However, aspartame-fed hypercholesterolemic zebrafish display acute death with swimming defects, suggesting putative brain damage induced by aspartame [4].…”

Section: Introductionmentioning

confidence: 97%

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Modified High-Density Lipoproteins by Artificial Sweetener, Aspartame, and Saccharin, Showed Loss of Anti-atherosclerotic Activity and Toxicity in Zebrafish

et al. 2014

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Safety concerns have been raised regarding the association of chronic consumption of artificial sweeteners (ASs) with metabolic disorders, especially in the heart and brain. There has been no information on the in vivo physiological effects of AS consumption in lipoprotein metabolism. High-dosage treatment (final 25, 50, and 100 mM) with AS (aspartame, acesulfame K, and saccharin) to human high-density lipoprotein (HDL) induced loss of antioxidant ability along with elevated atherogenic effects. Aspartame-treated HDL3 (final 100 mM) almost all disappeared due to putative proteolytic degradation. Aspartame- and saccharin-treated HDL3 showed more enhanced cholesteryl ester transfer activity, while their antioxidant ability was disappeared. Microinjection of the modified HDL3 exacerbated the inflammatory death in zebrafish embryos in the presence of oxLDL. These results show that AS treatment impaired the beneficial functions of HDL, resulting in loss of antioxidant and anti-atherogenic activities. These results suggest that aspartame and saccharin could be toxic to the human circulation system as well as embryonic development via impairment of lipoprotein function.

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Section: Cetp Activity Is Enhanced By As Treatmentsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 95%

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Modified High-Density Lipoproteins by Artificial Sweetener, Aspartame, and Saccharin, Showed Loss of Anti-atherosclerotic Activity and Toxicity in Zebrafish

et al. 2014

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“…Zebrafish were maintained at 28 ± 1 o C under a 14：10 hr light:dark cycle. After feeding for 4 weeks as designated in Table 1, blood (2 μl) was drawn and analyzed, as our previous report (8). Measurements of serum and hepatic parameters were carried out as described in Supplemental methods.…”

Section: In Vivo Test Using Hypercholesterolemic Zebrafishmentioning

confidence: 99%

“…In this study, we used a hyperlipidemic zebrafish (Danio rerio) model, in which hypercholesterolemia was induced by a high cholesterol diet (HCD) to mimic early atherogenesis and concurrent complications, as suggested by the Miller group (7). We recently reported that atherosclerosis and hypercholesterolemia were induced by feeding a HCD to adult zebrafish for several weeks (1,2,8). Among lipoproteins, low-density lipoprotein (LDL) is the major cholesterol carrier in the human blood.…”

Section: Introductionmentioning

confidence: 99%

1,8-cineole protected human lipoproteins from modification by oxidation and glycation and exhibited serum lipid-lowering and anti-inflammatory activity in zebrafish

Cho¹

2012

BMB Reports

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Phase‐Separated Liposomes Hijack Endogenous Lipoprotein Transport and Metabolism Pathways to Target Subsets of Endothelial Cells In Vivo

Arias-Alpizar

Papadopoulou

Rios

et al. 2023

Adv Healthcare Materials

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Plasma lipid transport and metabolism are essential to ensure correct cellular function throughout the body. Dynamically regulated in time and space, the well‐characterized mechanisms underpinning plasma lipid transport and metabolism offers an enticing, but as yet underexplored, rationale to design synthetic lipid nanoparticles with inherent cell/tissue selectivity. Herein, a systemically administered liposome formulation, composed of just two lipids, that is capable of hijacking a triglyceride lipase‐mediated lipid transport pathway resulting in liposome recognition and uptake within specific endothelial cell subsets is described. In the absence of targeting ligands, liposome‐lipase interactions are mediated by a unique, phase‐separated (“parachute”) liposome morphology. Within the embryonic zebrafish, selective liposome accumulation is observed at the developing blood‐brain barrier. In mice, extensive liposome accumulation within the liver and spleen – which is reduced, but not eliminated, following small molecule lipase inhibition – supports a role for endothelial lipase but highlights these liposomes are also subject to significant “off‐target” by reticuloendothelial system organs. Overall, these compositionally simplistic liposomes offer new insights into the discovery and design of lipid‐based nanoparticles that can exploit endogenous lipid transport and metabolism pathways to achieve cell selective targeting in vivo.

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Aspartame-fed zebrafish exhibit acute deaths with swimming defects and saccharin-fed zebrafish have elevation of cholesteryl ester transfer protein activity in hypercholesterolemia

Cited by 61 publications

References 37 publications

Modified High-Density Lipoproteins by Artificial Sweetener, Aspartame, and Saccharin, Showed Loss of Anti-atherosclerotic Activity and Toxicity in Zebrafish

Modified High-Density Lipoproteins by Artificial Sweetener, Aspartame, and Saccharin, Showed Loss of Anti-atherosclerotic Activity and Toxicity in Zebrafish

1,8-cineole protected human lipoproteins from modification by oxidation and glycation and exhibited serum lipid-lowering and anti-inflammatory activity in zebrafish

Phase‐Separated Liposomes Hijack Endogenous Lipoprotein Transport and Metabolism Pathways to Target Subsets of Endothelial Cells In Vivo

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