Asparaginase II of Saccharomyces cerevisiae: Characterization of a Mutation that Affects Expression in Rapidly Growing Cells

Asparaginase II of Saccharomyces cerevisiae: positive selection of two mutations that prevent enzyme synthesis

“…Asparaginase II is a yeast exoenzyme which is secreted into the cell wall in response to nitrogen limitation (5-9, 11, 12, 15-17, 20-22, 25). The genetics of Saccharomyces cerevisiae asparaginase II have been previously characterized with wild-type S. cerevisiae strains which either fail to synthesize the enzyme or exhibit a reduced synthetic capacity (5,11,12,20). Yeasts carrying the asp2 mutation can synthesize only 20 to 30% of the normal enzyme levels.…”