Asiatic acid abridges pre-neoplastic lesions, inflammation, cell proliferation and induces apoptosis in a rat model of colon carcinogenesis

Siddique, Aktarul Islam; Mani, Vijay; Renganathan, Senbagarani; Ayyanar, Rajagopal; Nagappan, Ananthi; Nalini, Namasivayam

doi:10.1016/j.cbi.2017.10.024

Cited by 18 publications

(9 citation statements)

References 65 publications

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“…Previous study also revealed that AA induced cells undergo S-G2/M phase arrest and apoptosis in human breast cancer MCF-7 and MDA-MB-231 cell lines [6]. Siddique et al suggested that AA induced apoptosis through decreasing the Bcl-2 and increasing the Bax, caspase-3 and -9 in a rat model of colon carcinogenesis [11].…”

Section: Discussionmentioning

confidence: 92%

“…It has been proposed that Asiatic acid has various pharmacological activities such as anti-inflammatory and antioxidant, as well as the potent anti-hypertensive, neuro and cardio-protective, antimicrobial, and antitumor activities [5]. Many studies had proved the anti-cancer effects in vitro or in vivo of AA in breast cancer, ovary cancer, colon cancer, gastric cancer, cholangiocarcinoma, glioblastoma, lung cancer, lymphoma, and melanoma [6,[11][12][13][14][15][16][17][18]. Our study corresponds with these researches and revealed the anti-cancer effect of AA in both dose and time dependent manners in cisplatin-resistance NPC cell lines (Figure 1b).…”

Section: Discussionmentioning

confidence: 99%

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Asiatic Acid, Extracted from Centella asiatica and Induces Apoptosis Pathway through the Phosphorylation p38 Mitogen-Activated Protein Kinase in Cisplatin-Resistant Nasopharyngeal Carcinoma Cells

Liu

Chuang

et al. 2020

Biomolecules

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Nasopharyngeal carcinoma (NPC) is an important issue in Asia because of its unique geographical and ethnic distribution. Cisplatin-based regimens are commonly the first-line used chemotherapy, but resistance and toxicities remain a problem. Therefore, the use of anticancer agents derived from natural products may be a solution. Asiatic acid (AA), extracted from Centella asiatica, was found to have anticancer activity in various cancers. The aim of this study is to examine the cytotoxic effect and mediated mechanism of AA in cisplatin-resistant NPC cells. The results shows that AA significantly reduce the cell viability of cisplatin-resistant NPC cell lines (cis NPC-039 and cis NPC-BM) in dose and time dependent manners caused by apoptosis through the both intrinsic and extrinsic apoptotic pathways, including altered mitochondrial membrane potential, activated death receptors, increased Bax expression, and upregulated caspase 3, 8, and 9. The Western blot analysis of AA-treated cell lines reveals that the phosphorylation of MAPK pathway proteins is involved. Further, the results of adding inhibitors of these proteins indicates that the phosphorylation of p38 are the key mediators in AA-induced apoptosis in cisplatin-resistant human NPC cells. This is the first study to demonstrate the AA-induced apoptotic pathway through the phosphorylation p38 in human cisplatin-resistant nasopharyngeal carcinoma. AA is expected to be another therapeutic option for cisplatin-resistant NPC because of the promising anti-cancer effect and fewer toxic properties.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Asiatic Acid, Extracted from Centella asiatica and Induces Apoptosis Pathway through the Phosphorylation p38 Mitogen-Activated Protein Kinase in Cisplatin-Resistant Nasopharyngeal Carcinoma Cells

Liu

Chuang

et al. 2020

Biomolecules

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“…The identification of bound primary antibody (immune) was carried out using a secondary antibody, which was inturn conjugated with horseradish peroxidase polymer for 30 min at room temperature. The immune precipitant was envisioned by treating with 3, 3`-diaminobenzidine (DAB), counterstaining with hematoxylin, and photographed using 40X magnification (AxioScope A1, Carl Zeiss, Jena, Germany) 19 .…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…After cleansing, the membranes were incubated with 1:2000 dilution of horse radish per oxidaseconjugated secondary antibody for two hours at 30 ⁰C. After satisfactory cleaning, the immunereactive proteins were envisioned by improved chemiluminescence detection reagents (Sigma) and quantified by Image J (1.51 f) (NIH, Bethesda, MD) 19 .…”

Section: Immunohistochemistrymentioning

confidence: 99%

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2021

IJPSR

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Zingerone is an active phenolic acid and the least pungent component of Zingiber officinale. It is known to exhibit different pharmacological properties which include anti-inflammatory, antioxidant, anticancer and antimicrobial activities. But, so far there has been no information available on the role of zingerone in experimental breast carcinogenesis. The objective of this experiment is to untwine the anticancer qualities of zingerone against dimethylbenz (a) anthracene (DMBA)-incited mammary carcinogenesis. To achieve this goal, female Sprague-Dawley rats were arbitrarily classified into six groups. Group 1 was notified as the control, groups 2-5 were given a single dose of DMBA (25 mg/kg b.wt, subcutaneously) in the 4th week. Along with DMBA, groups 3 (initium), 4 (post-initium) and 5 (entire period) rats received zingerone (20mg/kg b.wt. p.o) every day in different time periods during the experimental period of 16 weeks. Group 6 rats received 20 mg/kg b.wt. of zingerone alone. Cancer-bearing animal mammary tissue when evaluated by western blot showed increased immune expression patterns of ER, PR, HER2/ neu, cyclin D1 and also antiapoptotic protein (Bcl-2) and decreased expression of the proapoptotic proteins (P53, P21, Bax, caspases 3 and 9. When zingerone (20 mg/kg b. wt.) supplementation was continued for the entire duration of the experiment to DMBA-treated rats, the expression patterns of various tumor and apoptotic markers including the pathological modifications were switched back to essentially normal. Therefore, we conclude that zingerone could be considered as a powerful chemo preventive agent to counter DMBA incited breast carcinogenesis.

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“…AA exhibits a range of pharmacological properties, including anti-inflammatory, antibacterial ( Huang et al, 2011 ), antioxidant, cardioprotective, and neuroprotective activities ( Nagoor Meeran et al, 2018 ). AA treatment has been shown to suppress the growth of breast ( Hsu et al, 2004 ) and lungcancer cells ( Wu et al, 2017 ), without inducing substantial toxicity in non-malignant cells ( Siddique et al, 2017 ). As our preliminary studies have suggested that AA is additionally capable of inhibitingtongue cancer cell growth, we conducted the present study in an effort to better understand the mechanistic basis for the anti-cancer activity of this promising therapeutic compound.…”

Section: Introductionmentioning

confidence: 99%

Asiatic Acid Induces Endoplasmic Reticulum Stress and Activates the Grp78/IRE1α/JNK and Calpain Pathways to Inhibit Tongue Cancer Growth

Chen²,

Huang

et al. 2021

Front. Pharmacol.

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Asiatic acid (AA) has been shown to induce apoptotic death in a range of cancers, but the mechanisms whereby it can inhibit tongue cancer growth have yet to be clarified. Herein, we explored the effects of AA on tongue cancer cells and found that it induced their apoptotic death in vitro and in vivo, while additionally impairing xenograft tumor growth in vivo. From a mechanistic perspective, AA treatment was associated with increases in levels of calcium and the calcium- dependent protease calpain, and it further induced endoplasmic reticulum (ER) stress and consequent Grp78-related IRE1α and JNK phosphorylation, ultimately driving caspase-3 activation and apoptotic death. Together, these results highlight AA as a promising tool for the therapeutic treatment of tongue cancer in clinical practice.

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Asiatic acid abridges pre-neoplastic lesions, inflammation, cell proliferation and induces apoptosis in a rat model of colon carcinogenesis

Cited by 18 publications

References 65 publications

Asiatic Acid, Extracted from Centella asiatica and Induces Apoptosis Pathway through the Phosphorylation p38 Mitogen-Activated Protein Kinase in Cisplatin-Resistant Nasopharyngeal Carcinoma Cells

Asiatic Acid, Extracted from Centella asiatica and Induces Apoptosis Pathway through the Phosphorylation p38 Mitogen-Activated Protein Kinase in Cisplatin-Resistant Nasopharyngeal Carcinoma Cells

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Asiatic Acid Induces Endoplasmic Reticulum Stress and Activates the Grp78/IRE1α/JNK and Calpain Pathways to Inhibit Tongue Cancer Growth

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