Asian Sand Dust Particles Enhance the Development of Aspergillus fumigatus Biofilm on Nasal Epithelial Cells

Abstract

Background: Asian sand dust (ASD) and Aspergillus fumigatus are known risk factors for airway mucosal inflammatory diseases. Bacterial and fungal biofilms commonly coexist in chronic rhinosinusitis and fungus balls. We evaluated the effects of ASD on the development of A. fumigatus biofilm formation on nasal epithelial cells. Methods: Primary nasal epithelial cells were cultured with A. fumigatus conidia with or without ASD for 72 h. The production of interleukin (IL)-6, IL-8, and transforming growth factor (TGF)-β1 from nasal epithelial cells was determined by the enzyme-linked immunosorbent assay. The effects of ASD on A. fumigatus biofilm formation were determined using crystal violet, concanavalin A, safranin staining, and confocal scanning laser microscopy. Results: ASD and A. fumigatus significantly enhanced the production of IL-6 and IL-8 from nasal epithelial cells. By coculturing A. fumigatus with ASD, the dry weight and safranin staining of the fungal biofilms significantly increased in a time-dependent manner. However, the increased level of crystal violet and concanavalin A stain decreased after 72 h of incubation. Conclusions: ASD and A. fumigatus induced the production of inflammatory chemical mediators from nasal epithelial cells. The exposure of A. fumigatus to ASD enhanced the formation of biofilms. The coexistence of ASD and A. fumigatus may increase the development of fungal biofilms and fungal inflammatory diseases in the sinonasal mucosa.

Impact of Zinc Oxide on the Development of Aspergillus-Induced Maxillary Sinusitis Rabbit Model

“…The germination of conidia and filamentous growth of fungi with the interaction of polysaccharides in the extracellular matrix can develop biofilms. A. fumigatus can form biofilm on primary nasal epithelial cells and various hazardous conditions can exacerbate the development of fungal biofilms [ 6 , 14 ]. ZO can paralyze the mucociliary function or cause edema and hyperemia of sinus mucosa, which induce a favorable condition for fungal growth and the development of fungal biofilms [ 8 , 11 ].…”

“…In healthy mucosa with an intact innate immune defense system, fungal biofilms cannot develop with inhaled fungal conidia. In pathologic conditions, such as mucosal injury, exposure to air pollutants, or obstructed sinus ostium, can induce the development of fungal biofilms [ 5 , 6 ]. Biofilm formation in sinus mucosa could be an important factor in the development of recalcitrant chronic inflammatory disease.…”