Asian corn borer pheromone binding protein 3, a candidate for evolving specificity to the 12-tetradecenyl acetate sex pheromone

Allen, Jean E.; Wanner, Kevin W.

doi:10.1016/j.ibmb.2010.10.005

Cited by 43 publications

(62 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of these transcripts, 14 showed > 95% sequence identity to previously identified O . furnacalis gene products already represented in GenBank: 5 PBPs (Accession number: GU828024 to GU828028; 1 GOBP2 (DQ673101) and 8 ORs (AB467327, JX910526, JN169134, JN169136, JN169138, JN169142, JX910532, JX910533; [16,32]; remaining search results not shown). The additional 75 putative olfactory-related transcripts found in the current study were not previously described in O .…”

Section: Resultsmentioning

confidence: 99%

“…Specifically, regulation of PBP3 is biased for high expression in male compared to female antennae [16], and analogous male-biased expression was also shown for O . nubilalis OR3 , 4 , 5 and 6 [31].…”

Section: Discussionmentioning

confidence: 96%

“…Directional selection between orthologs of male Ostrinia nubilalis and O . furnacalis antennal expressed PBP3 was hypothesized to result from the evolution of selective binding between structurally distinct sex pheromones emitted by corresponding females of the same species [16]. Furthermore, neuron response to the Drosophila pheromone, cis -vaccenyl acetate (cVA), was shown to directly depend on the function of an OBP called LUSH [17,18] that acts to solubilize and transport cVA [19].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Male- and Female-Biased Gene Expression of Olfactory-Related Genes in the Antennae of Asian Corn Borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae)

Zhang

Coates

et al. 2015

PLoS ONE

View full text Add to dashboard Cite

The Asian corn borer (ACB), Ostrinia furnacalis (Guenée), is a destructive pest insect of cultivated corn crops, for which antennal-expressed receptors are important to detect olfactory cues for mate attraction and oviposition. Few olfactory related genes were reported in ACB, so we sequenced and characterized the transcriptome of male and female O. furnacalis antennae. Non-normalized male and female O. furnacalis antennal cDNA libraries were sequenced on the Illumina HiSeq 2000 and assembled into a reference transcriptome. Functional gene annotations identified putative olfactory-related genes; 56 odorant receptors (ORs), 23 odorant binding proteins (OBPs), and 10 CSPs. RNA-seq estimates of gene expression respectively showed up- and down-regulation of 79 and 30 genes in female compared to male antennae, which included up-regulation of 8 ORs and 1 PBP gene in male antennae as well as 3 ORs in female antennae. Quantitative real-time RT-PCR analyses validated strong male antennal-biased expression of OfurOR3, 4, 6, 7, 8, 11, 12, 13 and 14 transcripts, whereas OfurOR17 and 18 were specially expressed in female antennae. Sex-biases gene expression described here provides important insight in gene functionalization, and provides candidate genes putatively involved in environmental perception, host plant attraction, and mate recognition.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Male- and Female-Biased Gene Expression of Olfactory-Related Genes in the Antennae of Asian Corn Borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae)

Zhang

Coates

et al. 2015

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…ECB and ACB antennae were prepared for RNA extraction using previously published methods (48). Full-length ORs were amplified from male ACB, ECB(E), and ECB(Z) antennal cDNA using primers designed to the 3′ and 5′ UTRs.…”

Section: Methodsmentioning

confidence: 99%

“…qPCR was performed as described previously (48). ORs 1, 3, 4, and 5 primer sets spanned an intron, and the absence of PCR products resulting from contaminating genomic DNA was confirmed by gel electrophoresis and melting temperature analysis.…”

Section: Methodsmentioning

confidence: 99%

Single mutation to a sex pheromone receptor provides adaptive specificity between closely related moth species

Leary

Allen

Bunger

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

183

208

View full text Add to dashboard Cite

Sex pheromone communication, acting as a prezygotic barrier to mating, is believed to have contributed to the speciation of moths and butterflies in the order Lepidoptera. Five decades after the discovery of the first moth sex pheromone, little is known about the molecular mechanisms that underlie the evolution of pheromone communication between closely related species. Although Asian and European corn borers (ACB and ECB) can be interbred in the laboratory, they are behaviorally isolated from mating naturally by their responses to subtly different sex pheromone isomers, (E)-12- and (Z)-12-tetradecenyl acetate and (E)-11- and (Z)-11-tetradecenyl acetate (ACB: E12, Z12; ECB; E11, Z11). Male moth olfactory systems respond specifically to the pheromone blend produced by their conspecific females. In vitro, ECB(Z) odorant receptor 3 (OR3), a sex pheromone receptor expressed in male antennae, responds strongly to E11 but also generally to the Z11, E12, and Z12 pheromones. In contrast, we show that ACB OR3, a gene that has been subjected to positive selection (ω = 2.9), responds preferentially to the ACB E12 and Z12 pheromones. In Ostrinia species the amino acid residue corresponding to position 148 in transmembrane domain 3 of OR3 is alanine (A), except for ACB OR3 that has a threonine (T) in this position. Mutation of this residue from A to T alters the pheromone recognition pattern by selectively reducing the E11 response ∼14-fold. These results suggest that discrete mutations that narrow the specificity of more broadly responsive sex pheromone receptors may provide a mechanism that contributes to speciation.

show abstract

MOLECULAR CHARACTERIZATION, EXPRESSION PATTERNS, AND LIGAND‐BINDING PROPERTIES OF TWO ODORANT‐BINDING PROTEIN GENES FROM Orthaga achatina (BUTLER) (LEPIDOPTERA: PYRALIDAE)

Liu

et al. 2012

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

It is postulated that insect pheromone-binding proteins (PBPs) are involved in sex pheromone reception, while the general odorant-binding proteins (GOBPs) are involved in reception of the general odorants including plant volatiles. However, this functional specificity is not completely conclusive. In the present study, full-length sequences of two new OBP genes were molecularly identified as OachPBP1 and OachGOBP2 from Orthaga achatina, an important pest of the camphor tree Cinnamomum camphora. Quantification of transcript levels by qRT-PCR showed that the two genes highly expressed in antennae, with OachPBP1 male-biased and OachGOBP2 similar between sexes. These expression patterns are consistent with the generally proposed functions of PBPs and GOBPs. With the recombinant proteins obtained by a bacterial expression system, the binding specificity of these proteins was further investigated and compared using the competitive binding assay. OachPBP1 exhibited high binding affinities with all three putative sex pheromones and 10 pheromone analogs, supporting its role in pheromone reception. On the other hand, in addition to binding with some plant volatiles, OachGOBP2 surprisingly displayed similar or even higher binding affinities with the sex pheromones than OachPBP1. Therefore, we propose that OachGOBP2 might play roles in reception of sex pheromone. Additionally, plant volatiles farnesol and farnesene showed high binding with both OachGOBP2 and OachPBP1, suggesting that these volatile chemicals have regulatory functions in the behavior of O. achatina.

show abstract

Asian corn borer pheromone binding protein 3, a candidate for evolving specificity to the 12-tetradecenyl acetate sex pheromone

Cited by 43 publications

References 58 publications

Male- and Female-Biased Gene Expression of Olfactory-Related Genes in the Antennae of Asian Corn Borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae)

Male- and Female-Biased Gene Expression of Olfactory-Related Genes in the Antennae of Asian Corn Borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae)

Single mutation to a sex pheromone receptor provides adaptive specificity between closely related moth species

MOLECULAR CHARACTERIZATION, EXPRESSION PATTERNS, AND LIGAND‐BINDING PROPERTIES OF TWO ODORANT‐BINDING PROTEIN GENES FROM Orthaga achatina (BUTLER) (LEPIDOPTERA: PYRALIDAE)

Contact Info

Product

Resources

About