‘Ashvagandharishta’ prepared using yeast consortium from Woodfordia fruticosa flowers exhibit hepatoprotective effect on CCl4 induced liver damage in Wistar rats

Bhondave, Prashant; Devarshi, Prasad P.; Mahadik, Kakasaheb R.; Harsulkar, Abhay

doi:10.1016/j.jep.2013.10.025

Cited by 34 publications

(22 citation statements)

References 22 publications

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“…Additionally, liver is the most vulnerable to toxic chemical agents (Wang et al, 2007;Bhondave et al, 2014). Acute liver injury typically causes rapid development of hepatocellular dysfunction and has a poor prognosis.…”

Section: Introductionmentioning

confidence: 99%

Carbon tetrachloride-induced lethality in mouse is prevented by multiple pretreatment with zinc sulfate

et al. 2016

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-Carbon tetrachloride (CCl 4 ) is commonly used as a chemical inducer of experimental liver injury. Several compounds have been demonstrated to attenuate the hepatic damage caused by sublethal doses of CCl 4 . However, rescue from lethal toxicity of CCl 4 has not been reported. In the present study, we evaluated the protective effect of metallothionein (MT), an endogenous scavenger of free radicals, on CCl 4 -induced lethal toxicity of mice. To induce MT production in male ddY mice, we administered Zn (as ZnSO 4 ) at 50 mg/kg as a once-daily subcutaneous injection for 3 days prior to a single intraperitoneal administration of 4 g/kg CCl 4 . Animals were observed for mortality every 3 hr for 24 hr after CCl 4 injection. Liver damage was assessed by determining (in a subset of these mice) blood levels of alanine aminotransferase (ALT; a marker of liver injury) and liver histopathology at 6 hr after CCl 4 injection. Our results showed that three times pretreatment with Zn yielded > 40-fold induction of hepatic MT protein levels compared to control group. Zn pretreatment completely abolished the CCl 4 -induced mortality of mice. We also found that pretreatment of mice with Zn significantly decreased the ALT levels and reduced the histological liver damage as assessed at 6 hr post-CCl 4 . These findings suggest that prophylaxis with Zn protects mice from CCl 4 -induced acute hepatic toxicity and mortality, presumably by induction of radical-scavenging MT.

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Section: Introductionmentioning

confidence: 99%

Carbon tetrachloride-induced lethality in mouse is prevented by multiple pretreatment with zinc sulfate

et al. 2016

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“…Liver is one of the most important internal organs in human body with multiple of functions such as detoxification, protein synthesis, and production of biochemicals necessary for digestion etc [1] . In addition, liver is also the most vulnerable organ attacked by chemical toxic agents [2] . Acute liver injury is usually referred as the rapid development of hepatocellular dysfunction with a poor prognosis.…”

Section: Introductionmentioning

confidence: 99%

The Protective Effect of Esculentoside A on Experimental Acute Liver Injury in Mice

et al. 2014

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Inflammatory response and oxidative stress are considered to play an important role in the development of acute liver injury induced by carbon tetrachloride (CCl4) and galactosamine (GalN)/lipopolysaccharides (LPS). Esculentoside A (EsA), isolated from the Chinese herb phytolacca esculenta, has the effect of modulating immune response, cell proliferation and apoptosis as well as anti-inflammatory effects. The present study is to evaluate the protective effect of EsA on CCl4 and GalN/LPS-induced acute liver injury. In vitro, CCK-8 assays showed that EsA had no cytotoxicity, while it significantly reduced levels of TNF-α and cell death rate challenged by CCl4. Moreover, EsA treatment up-regulated PPAR-γ expression of LO2 cells and reduced levels of reactive oxygen species (ROS) challenged by CCl4. In vivo, EsA prevented mice from CCl4-induced liver histopathological damage. In addition, levels of AST and ALT were significantly decreased by EsA treatment. Furthermore, the mice treated with EsA had a lower level of TNF-α, Interleukin (IL)-1β and IL-6 in mRNA expression. EsA prevented MDA release and increased GSH-Px activity in liver tissues. Immunohistochemical staining showed that over-expression of F4/80 and CD11b were markedly inhibited by EsA. The western bolt results showed that EsA significantly inhibited CCl4-induced phosphonated IkBalpha (P-IκB) and ERK. Furthermore, EsA treatment also alleviated GalN/LPS-induced acute liver injury on liver enzyme and histopathological damage. Unfortunately, our results exhibited that EsA had no effects on CCl4-induced hepatocyte apoptosis which were showed by TUNEL staining and Bax, Caspase-3 and cleaved Caspase-3 expression. Our results proved that EsA treatment attenuated CCl4 and GalN/LPS-induced acute liver injury in mice and its protective effects might be involved in inhibiting inflammatory response and oxidative stress, but not apoptosis with its underlying mechanism associated with PPAR-γ, NF-κB and ERK signal pathways.

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“…Serum aminotransferase activity is considered a biochemical marker of liver injury (Bhondave et al, ). It is reported that increased serum LDH level is observed in liver injury caused by shock or hypoxic condition (Kotoh et al ., 2008).…”

Section: Resultsmentioning

confidence: 99%

“…Sepsis is a clinical indication of systemic inflammation associated with an infectious assault caused by endotoxins, fungi, ischemia or reperfusion, or tissue injury and is associated with inflammatory responses triggered by pathogen components, like lipopolysaccharide (LPS) that interact with toll‐like receptors (TLRs) (Wang et al, ). Lipopolysaccharide stimulates Kupffer cells to release pro‐inflammatory cytokines, like tumor necrosis factor (TNF)‐α, interleukin (IL)‐1 and IL‐6 (Calandra et al, ; Yang et al, ), and these cytokines lead to hepatic damage by regulating oxidative status and eventually organ damage (Bhondave et al, ). Tumor necrosis factor‐α is the principle mediator of endotoxin shock and also activates caspase‐dependent apoptotic signaling (Galanos and Freudenberg, ).…”

Section: Introductionmentioning

confidence: 99%

Wheatgrass‐Derived Polysaccharide Has Antiinflammatory, Anti‐Oxidative and Anti‐Apoptotic Effects on LPS‐Induced Hepatic Injury in Mice

Nepali

Lee

et al. 2017

Phytotherapy Research

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Hepatic injury occurs frequently during sepsis, and polysaccharides isolated from plants have been reported to have antiinflammatory and antioxidant effects in various models. However, the effect of wheatgrass-derived polysaccharide (WGP) has not been previously studied. In the present study, we investigated the effect of WGP on lipopolysaccharide (LPS)-induced hepatic injury in mice. Mice were pre-treated with WGP (100 or 200 mg/kg daily for 2 days) and then challenged with LPS (1 mg/kg, intraperitoneal), and sacrificed after 12 h. Wheatgrass-derived polysaccharide decreased serum aminotransferase levels and histological changes as compared with LPS-challenged mice. Wheatgrass-derived polysaccharide also significantly inhibited LPS-induced pro-inflammatory cytokine up-regulation and improved the oxidative status of liver tissues. Furthermore, these effects were found to be mediated by the suppression of the transcriptional activity of nuclear factor-kappa B (NF-κB), due to inhibitions of transforming growth factor beta (TGF-β)-activated kinase (TAK)-1 phosphorylation and inhibition of kappa B (IκB)-α degradation. In addition, WGP inhibited the activations of mitogen-activated protein kinases (MAPKs). Wheatgrass-derived polysaccharide also attenuated hepatic cell death by modulating caspase-3 and apoptosis associated mitochondrial proteins, such as, B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X (Bax). Taken together, WGP possesses antiinflammatory, anti-oxidant and anti-apoptotic activity and ameliorates LPS-induced liver injury in mice. Copyright © 2017 John Wiley & Sons, Ltd.

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‘Ashvagandharishta’ prepared using yeast consortium from Woodfordia fruticosa flowers exhibit hepatoprotective effect on CCl4 induced liver damage in Wistar rats

Cited by 34 publications

References 22 publications

Carbon tetrachloride-induced lethality in mouse is prevented by multiple pretreatment with zinc sulfate

Carbon tetrachloride-induced lethality in mouse is prevented by multiple pretreatment with zinc sulfate

The Protective Effect of Esculentoside A on Experimental Acute Liver Injury in Mice

Wheatgrass‐Derived Polysaccharide Has Antiinflammatory, Anti‐Oxidative and Anti‐Apoptotic Effects on LPS‐Induced Hepatic Injury in Mice

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