About 50% of 1,057 green ash (Fraxinus pennsylvanica) systematically sampled in the Great Plains and Rocky Mountain regions had substantial dieback (>10% of crown branches with dieback), and the average growth ring width during the last 20 years was 2.9 mm. The overall condition of the population was rated fair. Ash yellows phytoplasmas were identified at 102 of 106 sites throughout six U.S. states (North Dakota, South Dakota, Wyoming, Nebraska, Colorado, Kansas) and three Canadian provinces (Alberta, Saskatchewan, Manitoba). These phytoplasmas had not previously been known in Alberta, Saskatchewan, Manitoba, Wyoming, Colorado, or Kansas. Incidence of phytoplasmal detection ranged from 16% in Wyoming to 71% in South Dakota. Incidence varied in the range 41 to 67% across site types and crown dieback classes. Incidence was highest in rural plantings, in trees with the most crown dieback, and in larger diameter trees. No significant relationships were detected between presence of ash yellows phytoplasmas and radial growth rates of trees.