Asexual embryogenesis and plant regeneration in Quercus

Gingas, V. M.; Lineberger, R. Daniel

doi:10.1007/bf00046867

Cited by 69 publications

(53 citation statements)

References 15 publications

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“…Indeed our results show that, whatever the growth regulators in the culture medium, explants prepared from small translucent embryonic structures (STE) were more embryogenic and gave direct secondary embryos at higher frequency than explants prepared from more advanced embryonic structures (LWE). This confirmed that the capacity for secondary embryogenesis is dependent on the non-differentiated state of the tissues which progressively disappears during growth and tissue specialisation as observed when zygotic embryos of Quercus [5,13,16,22] and other species [29,35,43,47] are used as initial explants.…”

Section: Discussionsupporting

confidence: 74%

“…The multiplication of the embryogenic lines was first achieved from calli ageing on the same culture medium [12,16] or via successive transfers onto fresh culture media with different growth regulator supplements [12,14]. Embryogenic response from anthers and ovary tissues was also obtained using similar procedures [20].…”

Section: Introductionmentioning

confidence: 99%

“…Secondary embryogenesis on culture media without growth regulators has been reported for a number of species including Q. rubra [16], Q. suber [11,14], Q. acutissima [22] and Q. robur [9,34]. Fernández-Guijarro et al [14] showed that on these growth regulator-free media, the secondary embryogenesis is influenced by macronutrient composition.…”

Section: Introductionmentioning

confidence: 99%

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Histological investigation of the multiplication step in secondary somatic embryogenesis of Quercus robur L.

2001

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-Standardized explants composed of hypocotyl and root-tip were prepared from embryonic structures obtained from one embryogenic line of Quercus robur L. maintained by regular transfer onto a solidified reference medium composed of the MS mineral solutions, glucose (0.12 M), casamino acid (0.1%), and NAA (10.74 µM). The regeneration capacity from these explants were tested on the reference medium and on 2 alternative media in which the NAA (10.74 µM) was omitted or substituted for a combination of IBA (9.80 µM)/BAP (8.90 µM). Within 30 days, 4 types of responses were observed including direct and indirect secondary embryogenesis. In the direct pathway, somatic embryos arose from 3-4 epidermal cells following two different modes, depending on whether or not the formation of a meristematic mass preceded the initiation of the embryogenic process. In the indirect pathway the embryos were formed from clumps of mitotically active cells included in callus developed within the cortical tissues. Depending on their histological origin, the embryos exhibited differences in their structural organization which could influence their potential for further maturation and conversion into viable plantlets. Explants prepared from small translucent embryonic structures were more embryogenic and expressed the direct pathway of secondary embryogenesis at higher frequency than explants prepared from more advanced embryonic structures. On the culture medium without growth regulator, direct secondary embryogenesis was the exclusive response whereas on the culture medium with growth regulators added both direct and indirect secondary embryogenesis occurred. NAA favoured the direct secondary embryogenesis, while conversely, the IBA/BAP combination stimulated the indirect secondary embryogenesis. The results are discussed in reference to the PEDC concept (pre-embryogenic determined cells).

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Section: Discussionsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Histological investigation of the multiplication step in secondary somatic embryogenesis of Quercus robur L.

2001

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“…Gingas and Lineberger (1989) only acclimated three plantlets of Q. rubra, which died six months later. Ishii et al (1999) transferred 10 plantlets from somatic embryos of Q. serrata, eight of which survived.…”

Section: Acclimation Of Cork Oak Plantletsmentioning

confidence: 99%

Oak somatic and gametic embryos maturation is affected by charcoal and specific aminoacids mixture

Pintos¹,

Manzanera

Bueno³

2010

Ann. For. Sci.

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Abstract• Development of both somatic and gametic embryogenesis has many applications in clonal forestry and genetic improvement, for instance as mass-propagation of genetically improved plants and production of pure lines through doubled-haploid plant regeneration from gametic embryos.• The goal of this work was to improve growth, maturation and plantlet regeneration of cork oak (Quercus suber L.) embryos from both somatic and gametic origin.• Activated charcoal promoted a significant increase in growth in terms of relative size and weight of both somatic and doubled-haploid embryos, as well as a more efficient control of secondary somatic embryogenesis during development. A significant interaction was also observed with amino acid nutrition. While some amino acids (i.e., glutamine, arginine or asparagine) did not show significant differences with the controls, a mixture of these three amino acids or gamma amino butyric acid stimulated embryo growth. The highest survival rate during acclimation of plantlets from both somatic and doubled-haploid origin was obtained when the embryos had been previously cultured on basal medium with 3% sucrose and 1% activated charcoal for two months at 4 • C and germinated on medium supplemented with 6-benzylaminopurine and indole-3-butyric acid.• We obtained more than 900 cork oak plantlets acclimated from several embryogenic lines, with a high survival rate, demonstrating that this methodology is applicable for large scale plantlet production. We also report the first regeneration of doubled-haploid plantlets in cork oak. • Le développement des deux embryogenèses gamétique et somatique a de nombreuses applications dans la foresterie clonale et l'amélioration génétique, par exemple en tant que propagation en masse de plants génétiquement améliorés et la production de lignées pures par régénération de plants haploïdes doublés d'embryons gamétiques. Mots-clés• L'objectif de ce travail était d'améliorer la croissance, la maturation et la régénération des plantules des embryons de chêne-liège (Quercus suber L.) des deux origines gamétique et somatique.• Le charbon actif a favorisé une augmentation significative de la croissance en termes de taille et de poids des embryons haploïdes doublés et somatiques, ainsi qu'un contrôle plus efficace de l'embryogenèse somatique secondaire au cours du développement. Une interaction significative a également été observée avec la nutrition aminoacide. Alors que certains acides aminés (c'est-à-dire, la glutamine, l'arginine ou l'asparagine) ne montrent pas de différences significatives avec les témoins, un mélange de ces trois acides aminés ou l'acide butyrique amino gamma a stimulé la croissance de l'embryon. Le taux de survie le plus élevé au cours de l'acclimatation des plantules d'origine à la fois somatique et haploïde doublé, a été obtenu lorsque les embryons ont été auparavant cultivés sur milieu de base à 3 % de saccharose et de 1 % de charbon actif pendant deux mois à 4 • C et ont germé sur un milieu complété par la 6-benzylaminopurine et ...

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“…Some authors report for formation of embryogenic structures preceded by development of callus tissue (4,11). A direct formation of somatic embryos has also been observed (5,6,10). In the study presented an initial embryogenic reaction following the both types mentioned above could be observed.…”

Section: Resultsmentioning

confidence: 87%

Somatic Embryogenesis and Regeneration of Plantlets In Common Oak (Quercus RoburL.)

Tsvetkov

1998

Biotechnology & Biotechnological Equipment

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Asexual embryogenesis and plant regeneration in Quercus

Cited by 69 publications

References 15 publications

Histological investigation of the multiplication step in secondary somatic embryogenesis of Quercus robur L.

Histological investigation of the multiplication step in secondary somatic embryogenesis of Quercus robur L.

Oak somatic and gametic embryos maturation is affected by charcoal and specific aminoacids mixture

Somatic Embryogenesis and Regeneration of Plantlets In Common Oak (Quercus RoburL.)

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