Asexual Cell Genetics

Wenzel, G.

doi:10.1201/9781420049275.ptb

Cited by 3 publications

(3 citation statements)

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“…This finding contradicts the commonly held belief that plants regenerated without a passage through a callus phase are less prone to somaclonal variation than those emerging after a period in callus [15-17]. This view has generated a widespread preference for anther culture over embryo culture for both micropropagation and for the generation of doubled haploid progenies, widely used in both breeding and genetic analysis.…”

Section: Discussionmentioning

confidence: 85%

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et al. 2007

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Background: When plant tissue is passaged through in vitro culture, many regenerated plants appear to be no longer clonal copies of their donor genotype. Among the factors that affect this so-called tissue culture induced variation are explant genotype, explant tissue origin, medium composition, and the length of time in culture. Variation is understood to be generated via a combination of genetic and/or epigenetic changes. A lack of any phenotypic variation between regenerants does not necessarily imply a concomitant lack of genetic (or epigenetic) change, and it is therefore of interest to assay the outcomes of tissue culture at the genotypic level.

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Section: Discussionmentioning

confidence: 85%

Untitled

et al. 2007

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“…According to a common notion, plant regeneration via androgenesis should be less error prone than via embryogenesis due to the lack or hardly observed callus phase [ 64 , 65 ], which is responsible for the release of cellular control over mutagenesis generated i.e. by the motion of transposons agents possibly induced as the result of genomic DNA demethylation [ 66 ].…”

Section: Discussionmentioning

confidence: 99%

DNA methylation changes and TE activity induced in tissue cultures of barley (Hordeum vulgare L.)

Orłowska

Machczyńska

Oleszczuk

et al. 2016

J of Biol Res-Thessaloniki

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BackgroundIn vitro plant regeneration via androgenesis or somatic embryogenesis is capable of inducing (epi)mutations that may affect sexual progenies. While epimutations are associated with DNA methylation, mutations could be due to the movement of transposons. The common notion is that both processes are linked. It is being assumed that demethylation activates transposable elements (TEs). Analysis of methylation changes and their relation with TEs activation in tissue cultures requires uniquely derived donor plants (Ds), their regenerants (Rs) and respective progeny (Ps) that would allow discrimination of processes not related to changes introduced via in vitro cultures. Moreover, a set of methods (RP-HPLC, SSAP, and MSTD) is needed to study whether different TEs families are being activated during in vitro tissue culture plant regeneration and whether their activity could be linked to DNA methylation changes or alternative explanations should be considered.ResultsThe in vitro tissue culture plant regeneration in barley was responsible for the induction of DNA methylation in regenerants and conservation of the methylation level in the progeny as shown by the RP-HPLC approach. No difference between andro- and embryo-derived Rs and Ps was observed. The SSAP and MSTD approach revealed that Ds and Rs were more polymorphic than Ps. Moreover, Rs individuals exhibited more polymorphisms with the MSTD than SSAP approach. The differences between Ds, Rs and Ps were also evaluated via ANOVA and AMOVA.ConclusionsStressful conditions during plant regeneration via in vitro tissue cultures affect regenerants and their sexual progeny leading to an increase in global DNA methylation of Rs and Ps compared to Ds in barley. The increased methylation level noted among regenerants remains unchanged in the Ps as indicated via RP-HPLC data. Marker-based experiments suggest that TEs are activated via in vitro tissue cultures and that, independently of the increased methylation, their activity in Rs is greater than in Ps. Thus, the increased methylation level may not correspond to the stabilization of TEs movement at least at the level of regenerants. The presence of TEs variation among Ds that were genetically and epigenetically uniform may suggest that at least some mobile elements may be active, and they may mask variation related to tissue cultures. Thus, tissue cultures may activate some TEs whereas the others remain intact, or their level of movement is changed. Finally, we suggest that sexual reproduction may be responsible for the stabilization of TEs.

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“…Ferrie (2002) and Wenzel (1998) reviewed haploid plant generation and estimated over 2000 publications covering more than 250 species describing these techniques and their application. The main advantage of androgenesis is the production of homozygous lines in one generation (haploid production followed by chromosome doubling) which may save years of selfing to establish homozygosity.…”

Section: Androgenesis (Haploid Plant Generation)mentioning

confidence: 99%

Biotechnology in Horticulture: 100 Years of Application

McCown¹

2003

HortSci

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Asexual Cell Genetics

Cited by 3 publications

References 124 publications

Untitled

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DNA methylation changes and TE activity induced in tissue cultures of barley (Hordeum vulgare L.)

Biotechnology in Horticulture: 100 Years of Application

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