Ascorbyl free radical: A noninvasive marker of oxidative stress in human open-heart surgery

Pietri, Sylvia; Seguin, J; D’Arbigny, Pierre; Culcasi, Marcel

doi:10.1016/0891-5849(94)90131-7

Cited by 67 publications

(29 citation statements)

References 23 publications

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“…Vitamin C has a major function in addition to its role as an enzyme cofactor, it being a key chain-breaking antioxidant in the aqueous phase (35). Vitamin C, in the presence of increased free radical production, can be rapidly deleted by electron spin resonance in body fl uids (36) as observed in this study; in the presence of increased lipid peroxidation, the concentration of vitamin C did not increase in the vitamin C or NACϩvitamin C group. The mechanism of the antioxidant defense systems in the lung and their overlapping specifi c activities suggested normal pulmonary cellular function needs adequate redox balance (37).…”

Section: Discussionsupporting

confidence: 51%

Efficacy of Ascorbic Acid (Vitamin C) and/N-Acetylcysteine (NAC) Supplementation on Nutritional and Antioxidant Status of Male Chronic Obstructive Pulmonary Disease (COPD) Patients

Pirabbasi

Shahar

Manaf

et al. 2016

J Nutr Sci Vitaminol

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Summary Antioxidant therapy has a potential to be introduced as therapeutic modality for chronic obstructive pulmonary disease (COPD) patients. This study aimed to determine the effect of antioxidant supplementation [ascorbic acid and N-Acetylcysteine (NAC)] on nutritional and antioxidant status in male COPD patients. A parallel and single blind randomised controlled clinical trial (RCT) was conducted at two medical centers in Kuala Lumpur, Malaysia. Seventy-nine subjects were recruited and randomly divided into four trial arms (i.e., NAC, vitamin C, NACϩvitamin C and control groups) for six mo. The primary outcome was changes in body mass index by estimating power of 90% and signifi cance level of pϽ0.05. Repeated Measure ANOVA showed that there was a signifi cant interaction effect on BMI (pϭ0.046) and carbohydrate intake (pϭ0.030), especially in the NAC group. Plasma glutathione (GSH) increased signifi cantly in all intervention groups, especially in vitamin C (pϭ0.005). A single supplementation of NAC or vitamin C improved nutritional and antioxidant status of subjects.

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Section: Discussionsupporting

confidence: 51%

Efficacy of Ascorbic Acid (Vitamin C) and/N-Acetylcysteine (NAC) Supplementation on Nutritional and Antioxidant Status of Male Chronic Obstructive Pulmonary Disease (COPD) Patients

Pirabbasi

Shahar

Manaf

et al. 2016

J Nutr Sci Vitaminol

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“…Thus, ascorbyl radicals provide an appropriate biomarker during oxidative stress. In accordance with this, Pietri et al (1994) reported that ascorbyl free radical is a noninvasive marker of oxidative stress in human open-heart surgery. Duan et al (2009) employ an ascorbyl radical ESR spectrum as a biomarker to study the oxidative stress level of the influenza virus A FM1 subset-infected mouse.…”

Section: Discussionsupporting

confidence: 49%

The role of cyclooxygenase-derived oxidative stress in surgically induced lymphedema in a mouse tail model

Chang

Uen

Chou

et al. 2013

Pharmaceutical Biology

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Context: Oxidative stress may contribute to lymphedema and subsequent tissue damage. However, the causal role of oxidative stress in lymphedema remains unclear. Objective: We attempted to detect and identify the free radicals formed in lymphedema fluid and assessed the protective mechanisms and effects of specific enzyme inhibitors and natural antioxidants. Materials and methods: To study the level of postsurgical oxidative stress with lymphedema in a mouse tail model, we used an electron spin resonance (ESR) method and an ascorbyl radical's ESR spectrum as an oxidative stress biomarker. The drug-treatment group received an i.p. injection with indomethacin (2 mg/kg), baicalein (15 mg/kg), MK-886 (3 mg/kg), zileuton (6.25 mg/kg), diphenyleneiodonium (DPI; 1 mg/kg), sulforaphane (30 mg/kg), oryzanol (30 mg/ kg) or sesamol (30 mg/kg) once daily for 14 d from the day of operation. All animals were sacrificed on day 14. Results: Administration of indomethacin, sulforaphane, oryzanol and sesamol significantly suppressed both the tail volume (56.9%, 77.8%, 72.2% and 38.1% inhibition, respectively, p50.01) and ascorbyl radical signals (31.4%, 54.5%, 79.3% and 57.1% inhibition, respectively, p50.01), compared with the control mice. No significant differences were found between any of the baicalein, MK-886, or zileuton groups compared with the control. DPI suppressed the tail volume (25.9% inhibition, p50.01) but not the ascorbyl radical signals. Conclusion: This study showed that COX-derived oxidative stress plays a major role in the pathological mechanisms of surgically induced lymphedema. Indomethacin, sulforaphane, oryzanol and sesamol exhibit potent protective properties against surgically induced lymphedema.

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“…It has been shown that ascorbate depletion or its reaction with free radicals can be directly probed by direct detection of AFR-DMSO or AFR, respectively, in a variety of chronic or acute oxidative stress situations in animals (28,29,42) and humans (2,43,44). Altered ascorbic acid status has been reported in the mucosa (5) and plasma (27) of IBD patients.…”

Section: Discussionmentioning

confidence: 99%

“…Thawed samples were quickly introduced into a standard aqueous flat cell which was fitted within the cavity of a Bruker ESP 300 (Karlsruhe, Germany) X-band (9.8 GHz) ESR spectrometer equipped with a TM110 cavity and operating at a field modulation of 100-kHz and a microwave power of 10 mW. Spectral acquisition was initiated at room temperature 60 s after either the addition of DMSO or the complete thawing of the DEPMPO- was performed according to the literature (16,42,44). Free-radical concentrations were derived from double integrals of the simulated spectra obtained with either WinSim software (13) or the program of Rockenbauer and Korecz (48).…”

Section: Methodsmentioning

confidence: 99%

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Colitis and Colitis-Associated Cancer Are Exacerbated in Mice Deficient for Tumor Protein 53-Induced Nuclear Protein 1

Gommeaux

Cano

García

et al. 2007

Molecular and Cellular Biology

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Tumor protein 53-induced nuclear protein 1 (TP53INP1) is an antiproliferative and proapoptotic protein involved in cell stress response. To address its physiological roles in colorectal cancer and colitis, we generated and tested the susceptibility of Trp53inp1-deficient mice to the development of colorectal tumors induced by injection of the carcinogen azoxymethane followed by dextran sulfate sodium (DSS)-induced chronic colitis. Trp53inp1-deficient mice showed an increased incidence and multiplicity of tumors compared to those of wild-type (WT) mice. Furthermore, acute colitis induced by DSS treatment was more severe in Trp53inp1-deficient mice than in WT mice. Treatment with the antioxidant N-acetylcysteine prevented colitis and colitisassociated tumorigenesis more efficiently in WT mice than in Trp53inp1-deficient mice, suggesting a higher oxidative load in the latter. Consistently, we demonstrated by electron spin resonance and spin trapping that colons derived from deficient mice produced more free radicals than those of the WT during colitis and that the basal blood level of the antioxidant ascorbate was decreased in Trp53inp1-deficient mice. Collectively, these results indicate that the oxidative load is higher in Trp53inp1-deficient mice than in WT mice, generating a more-severe DSS-induced colitis, which favors development of colorectal tumors in Trp53inp1-deficient mice.

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Ascorbyl free radical: A noninvasive marker of oxidative stress in human open-heart surgery

Cited by 67 publications

References 23 publications

Efficacy of Ascorbic Acid (Vitamin C) and/N-Acetylcysteine (NAC) Supplementation on Nutritional and Antioxidant Status of Male Chronic Obstructive Pulmonary Disease (COPD) Patients

Efficacy of Ascorbic Acid (Vitamin C) and/N-Acetylcysteine (NAC) Supplementation on Nutritional and Antioxidant Status of Male Chronic Obstructive Pulmonary Disease (COPD) Patients

The role of cyclooxygenase-derived oxidative stress in surgically induced lymphedema in a mouse tail model

Colitis and Colitis-Associated Cancer Are Exacerbated in Mice Deficient for Tumor Protein 53-Induced Nuclear Protein 1

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