Ascorbic Acid Uptake and Regulation of Type I Collagen Synthesis in Cultured Vascular Smooth Muscle Cells

Qiao, Huan; Bell, Jason; Juliao, Saul F.; Li, Liying; May, James M.

doi:10.1159/000135661

Cited by 48 publications

(40 citation statements)

References 68 publications

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“…Ascorbic acid, which is an antioxidant vitamin, is also reported to affect collagen metabolism in fibroblasts [43][44][45]. The amount of collagen secreted, however, was evaluated in the presence of ascorbic acid since ascorbic acid is essential for collagen maturation and secretion [46].…”

Section: Discussionmentioning

confidence: 99%

Effect of Selective Estrogen Receptor Modulator/Raloxifene Analogue on Proliferation and Collagen Metabolism of Tendon Fibroblast

Irie

Takahata

Majima

et al. 2010

Connective Tissue Research

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The selective estrogen receptor modulator raloxifene is therapeutically beneficial for postmenopausal connective tissue degradation, such as osteoporosis, vascular sclerosis, and dermal degradation; however, the effects of raloxifene on postmenopausal tendon metabolism have not been clarified. In this study, we investigated the effects of raloxifene analogue (LY117018) on cell proliferation and collagen metabolism using cultured rat Achilles tendon fibroblasts. 17beta-Estradiol (E(2); 10(-11)-10(-9) M) and LY117018 (10(-9)-10(-7) M) had no significant effects on tendon fibroblast proliferation, based on a BrdU (5-bromo-2'-deoxyuridine) incorporation assay (24 hr) and a WST-8 colorimetric assay (2 or 6 days). Neither E(2) nor LY117018 significantly altered the expression of type I collagen, which is a main component of the tendon extracellular matrix (ECM), whereas both E(2) and LY117018 significantly increased the expression of matrix metalloproteinase (MMP)-13, which is responsible for tendon collagen degradation in rat. Also, both E(2) and LY117018 increased the expression of type III collagen and elastin, which are minor components of tendon ECM, but are considered to govern the elastic properties of tendons. These changes in collagen and MMP induced by either E(2) or LY117018 were attenuated by the estrogen receptor alpha blocker ICI 182,780. The results of this study suggest that postmenopausal estrogen deficiency might downregulate tendon collagen turnover and decrease tendon elasticity. Further, raloxifene treatment might restore these changes to premenopausal levels.

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Section: Discussionmentioning

confidence: 99%

Effect of Selective Estrogen Receptor Modulator/Raloxifene Analogue on Proliferation and Collagen Metabolism of Tendon Fibroblast

Irie

Takahata

Majima

et al. 2010

Connective Tissue Research

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“…It has been shown that ferric Cygb (Cygb(Fe 3+ )) can be reduced by cellular reductants such as ascorbate (Asc)[23-25]. Asc concentrations are in the range of tens of micromolar in human serum [26-28], ~0.1 mM in vascular smooth muscle cells [29] in the presence of 50 μM extracellular Asc, ~300 μM in rat liver and brain cells [30], and 0.5-1 mM in human fibroblasts and endothelium[31, 32]. In the presence of O 2 , reduced Cygb binds O 2 to form oxyCygb that rapidly reacts with NO generating ferric Cygb.…”

Section: Introductionmentioning

confidence: 99%

Effect of temperature, pH and heme ligands on the reduction of Cygb(Fe3+) by ascorbate

Tong

Zweier

Huskey

et al. 2014

Archives of Biochemistry and Biophysics

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Cytoglobin (Cygb) plays a role in regulating vasodilation in response to changes in local oxygen concentration by altering the rate of nitric oxide (NO) metabolism. Because the reduction of Cygb(Fe3+) by a reductant is the control step for Cygb-mediated NO metabolism, we examined the effects of temperature, pH, and heme ligands on the Cygb(Fe3+) reduction by ascorbate (Asc) under anaerobic conditions. The standard enthalpy of Cygb(Fe3+) reduction by Asc was determined to be 42.4 ± 3.1 kJ/mol. The rate of Cygb(Fe3+) reduction increased ~6% per °C when temperature varied from 35 °C to 40 °C. The yield and the rate of Cygb(Fe3+) reduction significantly increases with pH (2-3 times per pH unit), paralleling the formation of the Asc ion (A2-) and the increased stability of reduced state of heme iron at high pH values. Heme ligand cyanide (CN-) decreased the yield and the rate of Cygb(Fe3+) reduction, but ligands CO and NO allowed the process of Cygb(Fe3+) reduction to continue to completion. Critical information is provided for modeling and prediction of the process of Cygb-mediated NO metabolism in vessels in a range of temperature and pH values.

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“…According to the literature, type III collagen plays an important role in remodeling, as well as in normal growth and development of connective tissue (Duncan et al 1984, Yen et al 1989. It has been reported that the production of collagens type I and III is enhanced by AA, even though more intensely in the case of type I collagen (Azulay et al 2003, Qiao et al 2009). …”

Section: Animal Proceduresmentioning

confidence: 99%

Histological evaluation of the periodontal ligament from aged wistar rats supplemented with ascorbic acid

Zanoni

Lucas

Trevizan

et al. 2013

An. Acad. Bras. Ciênc.

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Ascorbic acid (AA) is able to neutralize reactive oxygen species and is essential for collagen synthesis. In aging process oxidative stress is elevated. This study aims to investigate the effects of AA supplementation on the periodontal ligament (PL) of rats during aging. Twenty five rats were used and divided into groups: J90 (90-dayold control), E345 (345-day-old control), E428 (428-day-old control), EA345 (345-day-old supplemented with AA from 90-day-old on) and EA428 (428-day-old supplemented with AA from 90-day-old on). We analyzed the thickness, density of fibroblasts and blood vessels and collagen fibers types in the PL. In group J90 there was predominantly type III collagen fibers (87.64%). In animals supplemented with AA, the area filled by type I fibers (group EA345: 65.67%, group EA428: 52.23%) was higher than type III fibers. PL in group EA428 was thicker than the one observed in group E428 (P < 0.05). During natural aging process, AA promoted the maturation of collagen fibers and enhanced angiogenesis in periodontal ligament. One can conclude that the supplementation with AA represented a beneficial factor for the development of PL in aged rats.

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Ascorbic Acid Uptake and Regulation of Type I Collagen Synthesis in Cultured Vascular Smooth Muscle Cells

Cited by 48 publications

References 68 publications

Effect of Selective Estrogen Receptor Modulator/Raloxifene Analogue on Proliferation and Collagen Metabolism of Tendon Fibroblast

Effect of Selective Estrogen Receptor Modulator/Raloxifene Analogue on Proliferation and Collagen Metabolism of Tendon Fibroblast

Effect of temperature, pH and heme ligands on the reduction of Cygb(Fe3+) by ascorbate

Histological evaluation of the periodontal ligament from aged wistar rats supplemented with ascorbic acid

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