Ascorbic Acid Suppresses Germination and Dynamic States of Water in Wheat Seeds

Ishibashi, Yoshihiro; Iwaya‐Inoue, Mari

doi:10.1626/pps.9.172

Cited by 28 publications

(15 citation statements)

References 14 publications

(11 reference statements)

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“…its pro-oxidant activity), or ascorbate regulates pollen viability, pollination, fertilisation, ovule development or embryo development. A possibly related phenomenon is the inhibition of seed germination by treatment with relatively high concentrations of ascorbate (Tewary and Mookerjee, 1982;Cano et al, 1997;Ishibashi and Iwaya-Inoue, 2006), which we have confirmed in a wide variety of plant species (S. Bulley, unpublished data). Additionally, quiescent cells in meristems and mature desiccated seeds (including embryos) contain little or no reduced ascorbate and very low concentrations of oxidised dehydroascorbate (DeGara et al, 1997;Tommasi et al, 2001;De Tullio and Arrigoni, 2003).…”

Section: Discussionsupporting

confidence: 68%

Enhancing ascorbate in fruits and tubers through over‐expression of the l‐galactose pathway gene GDP‐l‐galactose phosphorylase

Bulley

Wright

Rommens

et al. 2011

Plant Biotechnology Journal

210

137

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Summary Ascorbate, or vitamin C, is obtained by humans mostly from plant sources. Various approaches have been made to increase ascorbate in plants by transgenic means. Most of these attempts have involved leaf material from model plants, with little success reported using genes from the generally accepted l‐galactose pathway of ascorbate biosynthesis. We focused on increasing ascorbate in commercially significant edible plant organs using a gene, GDP‐l‐galactose phosphorylase (GGP or VTC2), that we had previously shown to increase ascorbate concentration in tobacco and Arabidopsis thaliana. The coding sequence of Actinidia chinensis GGP, under the control of the 35S promoter, was expressed in tomato and strawberry. Potato was transformed with potato or Arabidopsis GGP genes under the control of the 35S promoter or a polyubiquitin promoter (potato only). Five lines of tomato, up to nine lines of potato, and eight lines of strawberry were regenerated for each construct. Three lines of tomato had a threefold to sixfold increase in fruit ascorbate, and all lines of strawberry showed a twofold increase. All but one line of each potato construct also showed an increase in tuber ascorbate of up to threefold. Interestingly, in tomato fruit, increased ascorbate was associated with loss of seed and the jelly of locular tissue surrounding the seed which was not seen in strawberry. In both strawberry and tomato, an increase in polyphenolic content was associated with increased ascorbate. These results show that GGP can be used to raise significantly ascorbate concentration in commercially significant edible crops.

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Section: Discussionsupporting

confidence: 68%

Enhancing ascorbate in fruits and tubers through over‐expression of the l‐galactose pathway gene GDP‐l‐galactose phosphorylase

Bulley

Wright

Rommens

et al. 2011

Plant Biotechnology Journal

210

137

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“…On the other hand, antioxidants are one of the new methods enhanced plant growth and development, increased photosynthetic pigments thereby increased chlorophyll and productivity as well, (Inskbashi and Iwaya, 2006). Ascorbic acid is known as growth regulating factor that influences many biological processes.…”

Section: Introductionmentioning

confidence: 99%

PARTIAL SUBSTITUTION OF CHEMICAL FERTILIZATION OF ROSELLE PLANT (Hibiscus sabdariffa L.) BY ORGANIC FERTILIZATION IN PRESENCE OF ASCORBIC ACID

Youssef

Mady

Ali

2014

Journal of Plant Production

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Two field experiments were conducted to evaluate the effect of different levels of organic fertilizer (5, 10 and 15 m 3 compost/fed.) when used with half dose of chemical fertilizer compare with full dose of chemical fertilizer in presence of ascorbic acid foliar spraying (0.0, 100 and 200ppm) on some growth parameters, yield and chemical constituents of roselle (Hibiscus sabdariffa L.) plants during 2011 and 2012 seasons. Interaction effects between ascorbic acid and the used fertilization treatments on growth and productivity were studied as well. The results showed that either treatment of half chemical fertilizer dose+15m 3 compost/fed. or full chemical fertilizer dose significantly induced the highest values of plant height, number of leaves, branches and fruits /plant, leaves dry weight/plant, fresh and dry weight of branches and sepals/plant, seed yield/plant, seed fixed oil per plant and per fed., sepals anthocyanin and vitamin-C content, leaf N, P, K, Fe, Zn and Mn uptake, leaf total carbohydrates, total sugars, total free amino acids, auxins, gibberellins and cytokinins content, but they decreased the values of leaf abscisic acid, peroxidase, catalase and superoxide dismutase contents. However, the highest number of leaves, branches and fruits/plant, the heaviest leaves dry weight/plant, the heaviest fresh and dry weights of sepals/plant, the highest seed fixed oil content per plant and per fed., the highest sepals vitamin-C contents, the highest leaf N, K, Fe and Mn uptake, the highest values of chlorophyll a&b, carotenoids, total carbohydrates, total sugars, total free amino acids, gibberellins and cytokinins as well as the lowest values of abscisic acid, peroxidase and superoxide dismutase were recorded by half chemical fertilizer dose+15m 3 compost/fed. treatment combined with 200ppm ascorbic acid. Besides, the highest sepals anthocyanin content was recorded by the combined treatment between half chemical fertilizer dose+10m 3 compost/fed. and 200ppm ascorbic acid in both seasons. Moreover, the tallest plants, the heaviest fresh and dry weight of branches/plant, the highest seed yield/plant, the highest leaf P and Zn uptake and the highest leaf auxins content of roselle plants were scored by the full chemical fertilizer dose combined with 200ppm ascorbic acid, followed by the combined treatment between half chemical fertilizer dose+15m 3 compost/fed. and 200ppm ascorbic acid in both seasons. Consequently, it could be safely treat roselle plants with the combined treatment between half dose of chemical fertilizer+15m 3 compost/fed. and ascorbic acid at 200 ppm for enhancing growth and productivity of this plant.

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“…H 2 O 2 also accelerates the germination of pea (Pisum sativum) seeds and stimulates the early growth of seedlings (Barba-Espin et al, 2010). In contrast, exogenously supplied antioxidant, which acts as an ROS scavenger, significantly suppressed the seed germination of several plant species (Ogawa and Iwabuchi, 2001;Ishibashi and Iwaya-Inoue, 2006). Production of H 2 O 2 during the early imbibition period has been demonstrated in seeds of soybean (Glycine max; Puntarulo et al, 1988), maize (Zea mays; Hite et al, 1999), wheat (Triticum aestivum; Caliskan and Cuming, 1998), and zinnia (Zinnia elegans; Ogawa and Iwabuchi, 2001); ROS produced after imbibition are assumed to play a role in seed germination.…”

mentioning

confidence: 99%

Reactive Oxygen Species Are Involved in Gibberellin/Abscisic Acid Signaling in Barley Aleurone Cells

et al. 2012

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Reactive oxygen species (ROS) act as signal molecules for a variety of processes in plants. However, many questions about the roles of ROS in plants remain to be clarified. Here, we report the role of ROS in gibberellin (GA) and abscisic acid (ABA) signaling in barley (Hordeum vulgare) aleurone cells. The production of hydrogen peroxide (H2O2), a type of ROS, was induced by GA in aleurone cells but suppressed by ABA. Furthermore, exogenous H2O2 appeared to promote the induction of α-amylases by GA. In contrast, antioxidants suppressed the induction of α-amylases. Therefore, H2O2 seems to function in GA and ABA signaling, and in regulation of α-amylase production, in aleurone cells. To identify the target of H2O2 in GA and ABA signaling, we analyzed the interrelationships between H2O2 and DELLA proteins Slender1 (SLN1), GA-regulated Myb transcription factor (GAmyb), and ABA-responsive protein kinase (PKABA) and their roles in GA and ABA signaling in aleurone cells. In the presence of GA, exogenous H2O2 had little effect on the degradation of SLN1, the primary transcriptional repressor mediating GA signaling, but it promoted the production of the mRNA encoding GAMyb, which acts downstream of SLN1 and involves induction of α-amylase mRNA. Additionally, H2O2 suppressed the production of PKABA mRNA, which is induced by ABA:PKABA represses the production of GAMyb mRNA. From these observations, we concluded that H2O2 released the repression of GAMyb mRNA by PKABA and consequently promoted the production of α-amylase mRNA, thus suggesting that the H2O2 generated by GA in aleurone cells is a signal molecule that antagonizes ABA signaling.

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Ascorbic Acid Suppresses Germination and Dynamic States of Water in Wheat Seeds

Cited by 28 publications

References 14 publications

Enhancing ascorbate in fruits and tubers through over‐expression of the l‐galactose pathway gene GDP‐l‐galactose phosphorylase

Enhancing ascorbate in fruits and tubers through over‐expression of the l‐galactose pathway gene GDP‐l‐galactose phosphorylase

PARTIAL SUBSTITUTION OF CHEMICAL FERTILIZATION OF ROSELLE PLANT (Hibiscus sabdariffa L.) BY ORGANIC FERTILIZATION IN PRESENCE OF ASCORBIC ACID

Reactive Oxygen Species Are Involved in Gibberellin/Abscisic Acid Signaling in Barley Aleurone Cells

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