Ascorbic acid phosphate stimulates type IV collagen synthesis and accelerates adipose conversion of 3T3-L1 cells

Ono, Masaaki; Aratani, Yasuaki; Kitagawa, Ikumi; Kitagawa, Yasuo

doi:10.1016/0014-4827(90)90096-s

Cited by 72 publications

(50 citation statements)

References 29 publications

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“…Studies by our research group have found a high-fat diet-induced adiposity reduction by VC supplementation in rats , Garcia-Diaz et al 2007 and modifications in adipocyte catecholamine-induced lipolysis (Garcia-Diaz et al 2009). In vitro studies have reported controversial VC effects on adipocyte differentiation (Ono et al 1990, Krieger-Brauer & Kather 1995, Galinier et al 2006.…”

Section: Discussionmentioning

confidence: 98%

Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytes

García-Díaz

Campión²,

Milagro³

et al. 2010

Journal of Molecular Endocrinology

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Antioxidant-based treatments are emerging as an interesting approach to possibly counteract obesity fat accumulation complications, since this is accompanied by an increased systemic oxidative stress. The aim of this study was to analyze specific metabolic effects of vitamin C (VC) on epididymal primary rat adipocytes. Cells were isolated and incubated for 72 h in culture medium, in the absence or presence of 1 . 6 nM insulin, within a range of VC concentrations (5-1000 mM).Glucose-and lipid-related variables as well as the secretion/expression patterns of several obesity-related genes were assessed. It was observed that VC dose dependently inhibited glucose uptake and lactate production, and also reduced glycerol release in both control and insulin-treated cells. Also, VC caused a dramatic concentration-dependent fall in leptin secretion especially in insulin-stimulated cells. In addition, VC (200 mM) induced Cdkn1a and Casp8, partially inhibited Irs3, and together with insulin drastically reduced Gpdh (listed as Gpd1 in the MGI database) gene expressions. Finally, VC and insulin down-regulatory effects were observed on extracellular and intracellular reactive oxygen species production respectively. In summary, this experimental assay describes a specific effect of VC in isolated rat adipocytes on glucose and fat metabolism, and on the secretion/expression of important obesity-related proteins.

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Section: Discussionmentioning

confidence: 98%

Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytes

García-Díaz

Campión²,

Milagro³

et al. 2010

Journal of Molecular Endocrinology

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“…F2-isoprostane was strongly correlated with obesity (72) Improvement in impaired acetylcholine-stimulated vasodilatation in obese subjects following VC infusion (67) An antioxidant supplementation (VE, VC and carotene) prevented rise in oxidative stress induced by lower exercise in young overweight adults (73) Increase in plasma vitamin C levels correlated with decline in plasma free radicals and increase in GSH levels following chronic VC administration (63) Adipocyte differentiation and lipolysis VC treatment stimulated spontaneous 3T3-L1 mouse pre-adipocyte differentiation (74,76) Evidence Detail Reference…”

Section: Evidencementioning

confidence: 99%

“…Lower GPDH gene expression and activity of mature adipocytes induced by VC treatment (30,33) Cellular growth stimulation of adipocytes by phosphate ascorbic acid treatment (74,75) Decreased lipolityc effect of cathecolamines and reduced basal lipolysis rate by VC treatment on adipose tissue from lean animals (28)…”

Section: Evidencementioning

confidence: 99%

“…Regarding VC effects in adipocytes in vitro, several authors have demonstrated this vitamin can act on differentiation, triglyceride accumulation, and lipolysis, although with controversial results (74,75). Specifically, it has been observed that VC stimulates the "spontaneous" differentiation of the 3T3-L1 preadipocytes cell line (74,76), consequently increasing the activity of the glycerol phosphate dehydrogenase (GPDH) enzyme and the intracellular accumulation of triglycerides, both of which are markers of conversion of pre-adipocytes into mature adipocytes.…”

Section: C) Adipocyte Differentiation and Lipolysismentioning

confidence: 99%

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Vitamin C in the Treatment and/or Prevention of Obesity

García-Díaz

Lopez-Legarrea

Quintero

et al. 2014

J Nutr Sci Vitaminol

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“…In normal white fat tissue and in lipomas, monovacuolar cells generally exhibited a distinctive pericellular basement membrane composed of type IV collagen and laminin, whereas heparan sulfate proteoglycan and fibronectin were almost completely missing (9). The synthesis and secretion of type IV collagen, entactin, and laminin were enhanced when adipose conversion of 3T3-Ll cells was stimulated by hormones (10,11). In studies of a series of stromal cell lines from mouse bone marrow, fibronectin and laminin were detected in all of the cell lines tested, yet 14F1.1 adipocytes exhibited a particularly prominent extracellular deposition (12).…”

Section: Introductionmentioning

confidence: 99%

Preadipocyte Screening by Laminin in Porcine Stromal Vascular Cell Cultures

Hausman

1998

Obesity Research

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YU, Z.K., G.J. HAUSMAN. Preadipocyte screening by laminin in porcine stromal vascular cell cultures. Obes Res. 1998;6:299-306. Objective: To determine if subpopulations of cells in stromal vascular (S-V) cultures could be segregated and separated based on affinity for laminin substratum. Research Methods and Procedures: S-V cells were seeded and allowed to attach for various times; 4 hours was found to be optimal for cell attachment. Cultures were rinsed after 4 hours of seeding, and S-V cells were divided into three subpopulations based on affinity for laminin: (1) cells that did not attach to laminin; (2) cells that had a low affinity for laminin; and (3) cells that had a high affinity for laminin. After 24 hours, cultures were either stained for the AD-3 antigen (a marker for preadipocytes), C/EBP-a (a terminal differentiation marker), or C/EBP-o (an early preadipocyte marker). Companion cultures were treated with various media for 9 days and stained with oil red-O. Results: Cells with a high affinity for laminin had the highest proportion of AD-3 and C/EBP-a positive cells and the highest proportion of fat cells after treatment with insulin ± dexamethasone. Cells with a low affinity for laminin had th~highest proportion of C/EBP-o cells and the highest proportion of fat cells after treatment with fetal bovine serum+dexamethasone, followed by insulin. Discussion: These results indicate that differentiating preadipocytes adhere to laminin to a much greater degree than do non-preadipocytes. Therefore, laminin-coated dishes can be used to screen S-V cells to produce preadipocyte or fibroblast-enriched S-V cultures.

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Ascorbic acid phosphate stimulates type IV collagen synthesis and accelerates adipose conversion of 3T3-L1 cells

Cited by 72 publications

References 29 publications

Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytes

Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytes

Vitamin C in the Treatment and/or Prevention of Obesity

Preadipocyte Screening by Laminin in Porcine Stromal Vascular Cell Cultures

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