Ascorbic Acid Monoglucoside as Antioxidant and Radioprotector

Abstract

Ascorbic acid monoglucoside (AsAG), a glucoside derivative of ascorbic acid, has been examined for its antioxidant and radioprotective abilities. AsAG neutralized 1, 1 diphenyl -2-picryl-hydrazyl (DPPH), a stable free radical in a concentration dependent manner thus indicating its antioxidant ability. AsAG protected mice liver tissues in vitro from peroxidative damage in lipids (measured as TBARS) resulting from 25Gy gamma irradiation. It also protected plasmid pBR322 DNA from gamma-radiation induced strand breaks as evidenced from studies on agarose gel electrophoresis of the plasmid DNA after radiation exposure. Oral administration of AsAG to mice prior to whole body gamma radiation exposure (4Gy) resulted in a reduction of radiation induced lipid peroxides in the liver tissue indicating in vivo radiation protection of membranes. Pulse radiolysis studies indicated that AsAG offered radioprotection by scavenging free radicals. The rate constants for the reactions OH and N(3) radicals with AsAG were determined to be 6.4 x 10(9) dm(3) mol(-1) s(-1) and 2.3 x 10(9) dm(3) mol(-1) s(-1), respectively at pH 7. It was observed that AsAG radicals undergo conjugation as the pH of the solution is raised to 11 in the case of a one-electron oxidation reaction. As the OH(*) radical adds to the ring, the conjugation effect starts appearing at pH 10.

A Combination of Pre- and Post-Exposure Ascorbic Acid Rescues Mice from Radiation-Induced Lethal Gastrointestinal Damage

“…Pretreatment with ascorbic acid is believed to be effective for attenuating radiation-induced GI damage because of its potent antioxidative effect [8]. It is considered that the production of reactive oxygen species (ROS) is evoked immediately after radiation exposure, and that ascorbic acid may scavenge these ROS.…”

Free radical scavenging reactions and antioxidant activities of silybin: Mechanistic aspects and pulse radiolytic studies

“…Briefly, the mixture contained 150 ng plasmid pUC18 in 2.0)10 (2 mol/dm 3 phosphate buffer, pH 7.4, in the presence or absence of 2.5)10 (5 dm 3 silybin. After soft X-ray irradiation at 50 Gy, the DNA samples were applied to 1% agarose gels in 0.5)TBE buffer consisting of 8.9)10 (2 mol/dm 3 Tris Borate/2.0)10 (3 mol/dm 3 EDTA, pH 8.3 [20], and electrophoresis was performed at 140 V for 315 min. The gels were stained with ethidium bromide and DNA bands were photographed and analysed.…”

Ascorbic acid glucoside reduces neurotoxicity and glutathione depletion in mouse brain induced by nitrotriazole radiosensitazer

“…[17,18] AA-2G produces anti-oxidant and radioprotective effects due to blocking the radiation-induced lipid peroxidation processes and strand breaks in DNA. [19][20][21] We have shown that AA-2G markedly protects from the decrease in blood leukocytes, thymocytes, splenocytes and bone marrow cells in healthy mice administered with high CPA dose (250 mg/kg). [22] The present study was undertaken to investigate the potential of the anti-oxidant ascorbic acid glucoside in comparison with ascorbic acid to modulate sanazole-induced neurotoxicity.…”