Ascorbic Acid Mitigates D-galactose-Induced Brain Aging by Increasing Hippocampal Neurogenesis and Improving Memory Function

Nam, Sung Min; Seo, Misun; Seo, Jin-Seok; Rhim, Hyewhon; Nahm, Sang-Soep; Cho, Ik‐Hyun; Chang, Byung-Joon; Kim, Hyeonjoong; Choi, Sun-Hye; Nah, Seung‐Yeol

doi:10.3390/nu11010176

Cited by 61 publications

(51 citation statements)

References 54 publications

(80 reference statements)

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“…D-gal impaired antioxidant enzyme activities and increased pro-inflammatory cytokines also decreases cell proliferation [14]. Moreover, a study of neuronal cell survival using BrdU and NeuN double-staining reveal that D-gal decreases neuronal cell survival by inducing oxidative damage and inflammation [47]. In this study, neurogenesis in the group given chrysin at 10 and 30 mg/kg did not differ from that in the vehicle group.…”

Section: Discussionmentioning

confidence: 47%

Chrysin Protects against Memory and Hippocampal Neurogenesis Depletion in D-Galactose-Induced Aging in Rats

et al. 2020

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The interruption of hippocampal neurogenesis due to aging impairs memory. The accumulation of D-galactose (D-gal), a monosaccharide, induces brain aging by causing oxidative stress and inflammation, resulting in neuronal cell damage and memory loss. Chrysin, an extracted flavonoid, has neuroprotective effects on memory. The present study aimed to investigate the effect of chrysin on memory and hippocampal neurogenesis in brains aged using D-gal. Male Sprague-Dawley rats received either D-gal (50 mg/kg) by i.p. injection, chrysin (10 or 30 mg/kg) by oral gavage, or D-gal (50 mg/kg) and chrysin (10 or 30 mg/kg) for 8 weeks. Memory was evaluated using novel object location (NOL) and novel object recognition (NOR) tests. Hippocampal neurogenesis was evaluated using Ki-67, 5-bromo-2′-deoxyuridine (BrdU), and doublecortin (DCX) immunofluorescence staining to determine cell proliferation, cell survival, and number of immature neurons, respectively. We found that D-gal administration resulted in memory impairment as measured by NOL and NOR tests and in depletions in cell proliferation, cell survival, and immature neurons. However, co-treatment with chrysin (10 or 30 mg/kg) attenuated these impairments. These results suggest that chrysin could potentially minimize memory and hippocampal neurogenesis depletions brought on by aging.

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Section: Discussionmentioning

confidence: 47%

Chrysin Protects against Memory and Hippocampal Neurogenesis Depletion in D-Galactose-Induced Aging in Rats

et al. 2020

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“…Physiologically, OPN promotes the expression of pro-survival genes via nuclear factor kappa-b (NF- K B) [53]. BDNF is the target gene of NF- K B. Pb-induced reduction of BDNF was observed in our present and previous studies, and ascorbic acid was shown to increase the expression of BDNF [20,24]. Along with the positive effect of ascorbic acid on BDNF expression, ascorbic acid is vital for neuronal differentiation and development, and myelin formation [54].…”

Section: Discussionmentioning

confidence: 56%

“…For histological evaluation, the procedures for Nissl staining and immunohistochemistry were used, as described in our previous studies [22,24]. Briefly, the remaining 12 offspring ( n = 12 per group) were anesthetized by urethane on PND21 and perfused transcardially with heparinized phosphate-buffered saline (PBS; 0.1 M, pH 7.4), followed by fixative (4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

Effects of Ascorbic Acid on Osteopontin Expression and Axonal Myelination in the Developing Cerebellum of Lead-Exposed Rat Pups

Nam

Seo

Nahm

et al. 2019

IJERPH

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Osteopontin (OPN) is a multi-functional protein that binds to integrin and calcium-binding phosphoprotein. OPN is required for normal neuronal development and its axonal myelination. We studied the combined effect of lead (Pb) and ascorbic acid treatment on OPN expression in the developing cerebellum. We randomly divided pregnant female rats into three groups: control, Pb (lead acetate, 0.3%, drinking water), and Pb plus ascorbic acid (PA; ascorbic acid, 100 mg/kg, oral intubation) groups. The blood level of Pb was significantly increased, while ascorbic acid reduced Pb levels in the dams and pups. At postnatal day (PND) 21, results from Nissl staining and OPN immunohistochemistry demonstrated that OPN was detected in the Purkinje cell layer in the cerebellum. Ascorbic acid treatment mitigated Pb exposure-induced reduction in the number of intact Purkinje cells and OPN immunoreactive Purkinje cells in the cerebellum of pups. In addition, Pb-induced reduction in the number of oligodendrocytes and myelin-associated glycoprotein is associated with the malformation of the myelin sheath. Ascorbic acid provided protection from Pb-induced impairments. Pb-induced structural deficits in the cerebellum resulted in functional deterioration observed during locomotive tests (bar holding test and wire mesh ascending test), while ascorbic acid ameliorated these harmful effects. Present results suggest that the change of OPN is associated with myelination in the developing cerebellum. The results also demonstrated that exposure to Pb is harmful, while ascorbic acid treatment is beneficial.

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“…33 Oxidative stress and microglial activation have been linked with various neurological and psychiatric disorders (Koga et al, 2019). 33 Vitamin C has also been shown to improve oxidative stress and neurogenesis in the hippocampus of rat model of ageing (Khalil et al, 2011;Nam et al, 2019). 16,34 All the reports suggest that the substances present in Tualang honey have beneficial effects on neurogenesis and have the potential to inhibit mitigate oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Tualang honey improves memory and hippocampal changes in adult offspring of prenatally stressed rats

Aziz¹,

Hasim²,

Zakaria³

et al. 2020

tjps

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INTRODUCTION: Prenatal stress has been shown to be associated with development of abnormal behaviour as well as disruption of learning and memory processing of spatial information in the offspring. METHODS: This study investigated whether alteration of memory, changes in the hippocampus morphology, levels of malondialdehyde (MDA) and N-methyl-D-aspartate (NMDA) receptor in the hippocampus of adult rat offspring following prenatal stress could be prevented by administration of Tualang honey in the pregnant dams.Twenty-four pregnant rats were randomized into control (C), stress group (S) and stress group treated with Tualang honey (TH). Twenty-four adult off spring were sacrificed following Novel Object Recognition Test. Their brains were removed and histolological changes, level of MDA and NMDA receptors in the hippocampus were determined. RESULTS: The offspring from TH group showed significant increase in preference index (p< 0.05) and improved hippocampal morphology compared to S group The group also demonstrated a significantly lower level of MDA and NMDA receptors (P<0.01; P<0.05 respectively) compared to S group. There were no differences in the parameters investigated between C and TH groups. DISCUSSION AND CONCLUSION: The study has shown that Tualang honey administration was associated with improvement in memory and morphology, levels of MDA and NMDA receptors in the hippocampus in the adult offspring following prenatal stress. The results suggest the protective role of Tualang in prenatally stressed rat offspring

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Ascorbic Acid Mitigates D-galactose-Induced Brain Aging by Increasing Hippocampal Neurogenesis and Improving Memory Function

Cited by 61 publications

References 54 publications

Chrysin Protects against Memory and Hippocampal Neurogenesis Depletion in D-Galactose-Induced Aging in Rats

Chrysin Protects against Memory and Hippocampal Neurogenesis Depletion in D-Galactose-Induced Aging in Rats

Effects of Ascorbic Acid on Osteopontin Expression and Axonal Myelination in the Developing Cerebellum of Lead-Exposed Rat Pups

Tualang honey improves memory and hippocampal changes in adult offspring of prenatally stressed rats

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