Ascorbic acid in Drosophila and changes during aging

Massie, Harold R.; Shumway, Mary E.; Whitney, Sandra J.P.; Sternick, Syamala M.; Aiello, Valerie R.

doi:10.1016/0531-5565(91)90037-m

Cited by 25 publications

(32 citation statements)

References 7 publications

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“…However, life-time feeding of ascorbic acid does not increase the life span of these two strains of flies [53]. Ascorbic acid appreciably minimized the cytogenetic toxicity of the organophosphorous pesticides Malathion and Rogor in D. melanogaster [54,55].…”

Section: Discussionmentioning

confidence: 98%

Paraquat-induced Oxidative Stress in Drosophila melanogaster: Effects of Melatonin, Glutathione, Serotonin, Minocycline, Lipoic Acid and Ascorbic Acid

et al. 2006

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The efficacy of melatonin, glutathione, serotonin, minocycline, lipoic acid and ascorbic acid in counteracting the toxicity of paraquat in Drosophila melanogaster was examined. Male Oregon wild strain flies were fed for 5 days with control food or food containing the test substance. They were transferred in groups of five to vials containing only filter paper soaked with 20 mM paraquat in 5% sucrose solution. Survival was determined 24 and 48 h later. All the substances assayed increased the survival of D. melanogaster. At equimolar concentrations (0.43 mM) melatonin was more effective than serotonin, lipoic acid and ascorbic acid. However, lower concentrations of glutathione (0.22 mM) and minocycline (0.05 mM) were as efficient as melatonin. The highest survival rate (38.6%) after 48 h of paraquat treatment was found with 2.15 mM of lipoic acid. No synergistic effect of melatonin with glutathione, serotonin, minocycline, lipoic acid and ascorbic acid was detected.

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Section: Discussionmentioning

confidence: 98%

Paraquat-induced Oxidative Stress in Drosophila melanogaster: Effects of Melatonin, Glutathione, Serotonin, Minocycline, Lipoic Acid and Ascorbic Acid

et al. 2006

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“…Further, we analyzed data on the survival of Drosophila melanogaster which had antioxidants added to the diet (Fleming et al, 1981 ;Massie et al, 1991). Application of the methods for transformation of survival curves , and use of a special computer program for evaluation and comparison of survival curves (Klemera and Doubal, in press), allowed us to determine the parameters R o and k of Gompertz mortality curves corresponding to the data on survival and statistical evaluation of differences in the corresponding parameters of survival curves.…”

Section: Applicationsmentioning

confidence: 99%

The effect of antioxidants and dietary restriction on mortality curves

Ďoubal

Klemera

1999

AGE

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The full exploitation of information contained in mortality curves offers a tool for direct verification of theories of aging. We have analyzed the behavior of mortality curves for middle and high age groups and have proposed a mathematical model of mortality correlated with the rate of aging. The model offers an explanation for the mutual relationships between mortality curves and suggests potential methodologies for determining experimental modification of the rate of aging. The applicability of this theory is demonstrated by analysis of the changes in mortality as it is influenced by dietary antioxidants or by a calorie restricted diet. Survival data taken from a variety of studies were used as primary information, with parameters of mortality curves being determined by computer-assisted analysis of the curves. These analyses support the hypothesis that a dominant role for free radicals exists in the control of aging in Drosophila. However, in mammals-mice and rats, the effects of antioxidants as well as caloric restriction on mortality curves do not indicate that these treatments alter the rates of aging.

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Section: Introductionmentioning

confidence: 99%

“…Ascorbate was reported to be present in various marine invertebrates (36–38) and in Drosophila melanogaster (39). In Drosophila , ascorbate was detected in flies raised on an apparent ascorbate-free diet, but no evidence for endogenous biosynthesis of the molecule was shown (39). Because no studies have recently explored the presence or possible biosynthesis of ascorbate in invertebrates, several authors have concluded that invertebrates are incapable of synthesizing this molecule (33,40,41).…”

Section: Introductionmentioning

confidence: 99%

The invertebrate Caenorhabditis elegans biosynthesizes ascorbate

Patananan¹,

Budenholzer²,

Pedraza³

et al. 2015

Archives of Biochemistry and Biophysics

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L-ascorbate, commonly known as vitamin C, serves as an antioxidant and cofactor essential for many biological processes. Distinct ascorbate biosynthetic pathways have been established for animals and plants, but little is known about the presence or synthesis of this molecule in invertebrate species. We have investigated ascorbate metabolism in the nematode Caenorhabditis elegans, where this molecule would be expected to play roles in oxidative stress resistance and as cofactor in collagen and neurotransmitter synthesis. Using high-performance liquid chromatography and gas-chromatography mass spectrometry, we determined that ascorbate is present at low amounts in the egg stage, L1 larvae, and mixed animal populations, with the egg stage containing the highest concentrations. Incubating C. elegans with precursor molecules necessary for ascorbate synthesis in plants and animals did not significantly alter ascorbate levels. Furthermore, bioinformatic analyses did not support the presence in C. elegans of either the plant or the animal biosynthetic pathway. However, we observed the complete 13C-labeling of ascorbate when C. elegans was grown with 13C-labeled Escherichia coli as a food source. These results support the hypothesis that ascorbate biosynthesis in invertebrates may proceed by a novel pathway and lay the foundation for a broader understanding of its biological role.

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Ascorbic acid in Drosophila and changes during aging

Cited by 25 publications

References 7 publications

Paraquat-induced Oxidative Stress in Drosophila melanogaster: Effects of Melatonin, Glutathione, Serotonin, Minocycline, Lipoic Acid and Ascorbic Acid

Paraquat-induced Oxidative Stress in Drosophila melanogaster: Effects of Melatonin, Glutathione, Serotonin, Minocycline, Lipoic Acid and Ascorbic Acid

The effect of antioxidants and dietary restriction on mortality curves

The invertebrate Caenorhabditis elegans biosynthesizes ascorbate

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