Ascorbic acid effect on the onset of cell proliferation in pea root

Abstract

The ability of ascorbic acid to induce cell proliferation of non‐cycling cells was investigated in quiescent embryo root of Pisum sativum L. cv. Lincoln, as well as in the active plantlet root meristem, where a minor portion of the cells is non‐proliferating. Quiescent embryo cells speeded up the G0–G1 transition during germination in the presence of ascorbic acid. In addition, proliferating cells present in the root tip of 3‐day‐old plantlets, arrested at the G1/S boundary by hydroxyurea, resumed the cycle earlier than the control, when treated with ascorbic acid. In contrast, ascorbic acid was unable to induce the proliferation of non‐cycling cells present in the active meristem. Therefore, these data suggest that the ability of ascorbic acid lo induce cell proliferation depends on the physiological status of the cell. In particular the data indicate that ascorbic acid is involved in cell proliferation as a factor necessary to enable already competent cells to progress through the cell cycle phases, but not as a factor able to induce non‐competent cells to overcome proliferation arrest.

Generation and properties of ascorbic acid-overproducing transgenic tobacco cells expressing sense RNA for l-galactono-1,4-lactone dehydrogenase

“…AsA is an essential factor that is responsible for regulation of the cell division cycle (Liso et al 1984(Liso et al , 1988Innocenti et al 1990;Citterio et al 1994). We previously reported that cell division was restrained in AsA-deficient transgenic tobacco BY-2 cells (Tabata et al 2001).…”

Amelioration of salinity tolerance in Solanum tuberosum L. by exogenous application of ascorbic acid

“…In this regard, several studies have indicated possible roles of ascorbic acid. Exogenously applied ascorbic acid was suggested to be utilized in cell metabolism and to enhance the cell division efficacy of competent cells (Citterio et al 1994). The direct effect of salt was reported to alter the structure of photosynthetic membranes (Fidalgo et al 2004).…”

Rooting hastened in onions by ascorbate and ascorbate free radical

“…Very recently, Citterio et al (1994) reported a stimulation of the onset of cell proliferation in pea roots. They suggest that ASC accelerates the beginning of S phase by speeding up the transfer of Fe 2+ from ferritin to deoxyribonucleotide reductase.…”