Ascorbic acid and urate in human seminal plasma: determination and interrelationships with chemiluminescence in washed semen

Thiele, Jens J.; Freisleben, Hans-Joachim; Fuchs, Jürgen; Ochsendorf, F. R.

doi:10.1093/humrep/10.1.110

Cited by 146 publications

(99 citation statements)

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“…In humans, the seminal acid ascorbic levels correlate positively with the percentage of morphologically normal spermatozoa [45] and negatively with DNA fragmentation index [46]. This finding supports the therapeutic use of vitamin C in infertile males.…”

Section: Vitamin C (Ascorbic Acid)supporting

confidence: 60%

Role of Antioxidants in the Treatment of Male Infertility

Gadallah¹

2018

SMOAJ

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Section: Vitamin C (Ascorbic Acid)supporting

confidence: 60%

Role of Antioxidants in the Treatment of Male Infertility

Gadallah¹

2018

SMOAJ

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“…Therefore, increased lipid peroxidation is an indication of high level of ROS (Thiele et al, 1995) and used extensively to study oxidative stress (Huggett et al, 1992). A significant increase (p< 0.05) in lipid peroxidation was recorded in C. idella after 14 days exposure of bisphenol-A compared to control group.…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress and histopathologic biomarkers of exposure to bisphenol-A in the freshwater fish, Ctenopharyngodon idella

Faheem

Lone

2018

Braz. J. Pharm. Sci.

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Bisphenol-A (BPA) belongs to the family of endocrine disrupting chemicals (EDCs) and it is used in the production of polycarbonate plastic and epoxy resins. The reproductive toxicity of BPA is well documented but it also exerts its toxic effects through multiple pathways especially by inducing a state of oxidative stress and causing damage to the vital organs. In the present study, histopathologic and oxidative damage caused by BPA in liver and kidneys of fresh water cyprinid, Ctenopharyngodon idella was evaluated. LC 50 of BPA for Ctenopharyngodon idella was determined by probit regression analysis. Fish were exposed to a sublethal concentration of BPA i.e. 3.2 ppm (1/2 LC 50 ) for 14 days. Histologic studies revealed that BPA caused degenerative changes in liver and kidneys and exposure of sublethal concentration of BPA caused oxidative damage in both organs. Lipid peroxidation significantly increased in liver and kidneys of treated group. Catalase activity and reduced glutathione content significantly decreased in the group exposed to BPA compared to control and glutathione-S-transferase activity increased significantly in both organs exposed to the sublethal concentration of BPA. From this study it is concluded that BPA caused toxic effects in fish species by changing oxidative balance and damaging the vital organs.

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“…1,7,10, [17][18][19] Seminal fluid also contains non-enzymatic antioxidants (ascorbic acid, a-tocopherol, pyruvate, glutathione, Lcarnitine, taurine and hypotaurine) [20][21][22][23] which constitute the bulk of seminal antioxidant capacity. In addition, urate, 24 pyruvate, 11,25 albumin, beta carotenes and ubiquinol 26 have been detected in seminal plasma.…”

Section: Relationship Between Oxidative Stress and Sperm Dysfunctionmentioning

confidence: 99%

Antioxidant therapy in male infertility: fact or fiction?

Zini¹,

Alhathal²

2011

Asian J Androl

109

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Infertile men have higher levels of semen reactive oxygen species (ROS) than do fertile men. High levels of semen ROS can cause sperm dysfunction, sperm DNA damage and reduced male reproductive potential. This observation has led clinicians to treat infertile men with antioxidant supplements. The purpose of this article is to discuss the rationale for antioxidant therapy in infertile men and to evaluate the data on the efficacy of dietary and in vitro antioxidant preparations on sperm function and DNA damage. To date, most clinical studies suggest that dietary antioxidant supplements are beneficial in terms of improving sperm function and DNA integrity. However, the exact mechanism of action of dietary antioxidants and the optimal dietary supplement have not been established. Moreover, most of the clinical studies are small and few have evaluated pregnancy rates. A beneficial effect of in vitro antioxidant supplements in protecting spermatozoa from exogenous oxidants has been demonstrated in most studies; however, the effect of these antioxidants in protecting sperm from endogenous ROS, gentle sperm processing and cryopreservation has not been established conclusively. Keywords: antioxidant; male infertility; oxidative stress; sperm DNA fragmentation; vitamins RELATIONSHIP BETWEEN OXIDATIVE STRESS AND SPERM DYSFUNCTION Seminal oxidative stress (OS) results from an imbalance between reactive oxygen species (ROS) production and ROS scavenging by seminal antioxidants. Seminal OS is believed to be one of the main factors in the pathogenesis of sperm dysfunction and sperm DNA damage in male infertility.1-4 Indeed, it is estimated that 25% of infertile men possess high levels of semen ROS, whereas fertile men do not have high levels of semen ROS.1,4-6 Although a controlled production of these ROS is required for sperm physiology (sperm hyperactivation, capacitation and acrosome reaction) and for natural fertilization, [7][8][9] the excessive production of ROS by immature germ cells and leukocytes causes sperm dysfunction (lipid peroxidation, loss of motility and sperm DNA damage). 9,10 Spermatozoa are particularly susceptible to oxidative injury due to the abundance of plasma membrane polyunsaturated fatty acids. [10][11][12] These unsaturated fatty acids provide fluidity that is necessary for membrane fusion events (e.g., the acrosome reaction and sperm-egg interaction) and for sperm motility. However, the unsaturated nature of these molecules predisposes them to free radical attack and ongoing lipid peroxidation throughout the sperm plasma membrane. Once this process has been initiated, accumulation of lipid peroxides occurs on the sperm surface (this results in loss of sperm motility) and oxidative damage to DNA can ensue. 13,14SEMINAL ANTIOXIDANT CAPACITY AND SPERM DYSFUNCTION Seminal plasma and spermatozoa themselves are well endowed with an array of protective antioxidants to protect spermatozoa from OS, especially, at the post-testicular level. 6,15,16 Seminal plasma contains a number of high-molecular weigh...

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Ascorbic acid and urate in human seminal plasma: determination and interrelationships with chemiluminescence in washed semen

Cited by 146 publications

References 0 publications

Role of Antioxidants in the Treatment of Male Infertility

Role of Antioxidants in the Treatment of Male Infertility

Oxidative stress and histopathologic biomarkers of exposure to bisphenol-A in the freshwater fish, Ctenopharyngodon idella

Antioxidant therapy in male infertility: fact or fiction?

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