Ascorbic acid and amino acid values in the aqueous humor of a patient with Lowe's syndrome

Hayasaka, Seiji; Yamada, Tetsuya; Nitta, Koji; Kaji, Yoshihiro; Hiraki, Shigeyoshi; Tachinami, K; Matsumoto, Masayuki; Yamamoto, Shuichi; Yamamoto, Shuko

doi:10.1007/bf00941762

Cited by 3 publications

(3 citation statements)

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“…Lower levels of vitamin C in aqueous humor may be associated with glaucoma, including in patients with primary open angle glaucoma and secondary glaucoma [8, 9, 16]. Finally, patients with Lowe’s syndrome and exfoliation syndrome have significantly lower levels of ascorbic acid in aqueous humor than age-matched controls [6, 8]. These findings suggest that vitamin C concentrations may provide clues to the pathogenesis and treatment of several ocular diseases.…”

Section: Discussionmentioning

confidence: 99%

“…Vitamin C concentrations in aqueous humor are also lower in patients with various ophthalmic diseases. For example, the concentration of vitamin C in the anterior chamber has been reported lower in patients with Lowe’s syndrome and exfoliation syndrome than in age-matched controls [6–8]. Moreover, reduced levels of vitamin C in aqueous humor may be associated with glaucoma [8, 9].…”

Section: Introductionmentioning

confidence: 99%

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Ascorbic acid concentrations in aqueous humor after systemic vitamin C supplementation in patients with cataract: pilot study

et al. 2017

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BackgroundTo measure ascorbic acid concentration in aqueous humor of patients with cataract after oral or intravenous vitamin C supplementation.MethodsForty-two eyes of 42 patients with senile cataract who underwent uncomplicated cataract surgery were enrolled. Patients (n = 14 each) were administered oral vitamin C (2 g), intravenous vitamin C (20 g) or no treatment (control group) on the day before surgery. Samples of aqueous humor (0.1 cm3) were obtained by anterior chamber aspiration at the beginning of surgery and stored at −80 °C. Ascorbic acid concentration in aqueous humor was measured by high-pressure liquid chromatography.ResultsThe mean age at surgery was 62.5 years, with no difference among the three groups. The mean ± standard deviation concentrations of ascorbic acid in aqueous humor in the control and oral and intravenous vitamin C groups were 1347 ± 331 μmol/L, 1859 ± 408 μmol/L and 2387 ± 445 μmol/L, respectively. Ascorbic acid concentration was significantly lower in the control than in the oral (P < 0.01) and intravenous (P < 0.001) vitamin C groups and was significantly higher in the intravenous than in the oral vitamin C group (P < 0.05).ConclusionsAscorbic acid concentration in aqueous humor is increased by systemic vitamin C supplementation, with intravenous administration being more effective than oral administration.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ascorbic acid concentrations in aqueous humor after systemic vitamin C supplementation in patients with cataract: pilot study

et al. 2017

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“…Significant variations in aqueous levels of ascorbic acid have been reported in different ophthalmic conditions. Patients with exfoliation syndrome and Lowe's syndrome have lower levels of ascorbic acid in the aqueous humour when compared with age‐matched controls 22,25 . Regarding topical medications, corticosteroids seem to decrease ascorbic acid concentration in the aqueous humour 26 .…”

Section: Discussionmentioning

confidence: 97%

Ascorbic acid concentration is reduced in the secondary aqueous humour of glaucomatous patients

Leite

Prata

Kera

et al. 2009

Clinical Exper Ophthalmology

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Background: We aimed to evaluate the ascorbic acid concentration in secondary aqueous humour (AH) from glaucomatous patients and to compare it with primary AH from primary open‐angle glaucoma patients and non‐glaucomatous patients. Methods: Primary AH samples were prospectively obtained from clinically uncontrolled primary open‐angle glaucoma patients and senile cataract patients (controls) prior to trabeculectomy and cataract surgery. Secondary AH samples were obtained from eyes with previous intraocular surgery, prior to trabeculectomy or cataract surgery. AH (0.1 mL) was aspirated by inserting a 26‐gauge needle into the anterior chamber just before surgery and then immediately stored at −80°C. The ascorbic acid concentration was determined in a masked fashion by high‐pressure liquid chromatography. Results: A total of 18 patients with senile cataract, 16 glaucomatous patients with primary AH (no previous intraocular surgery) and 11 glaucomatous patients with secondary AH (previous intraocular surgery) were included. There was no difference in mean age between groups (P = 0.15). The mean ± standard deviation concentration of ascorbic acid in the secondary AH from glaucomatous patients (504 ± 213 µmol/L [95% confidence interval {CI}, 383–624]) was significantly lower than the concentration of ascorbic acid found in the primary aqueous of primary open‐angle glaucoma (919 ± 427 µmol/L [95% CI, 709–1128]) and control patients (1049 ± 433 µmol/L [95% CI, 848–1249]; P < 0.01, Kruskal‐Wallis test). Conclusions: The ascorbic acid concentration in secondary AH of glaucomatous patients was approximately twofold lower in comparison with primary AH of glaucomatous and cataract patients. The implications of a reduced concentration of ascorbic acid in the secondary AH deserve further investigation.

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Determination of Oxidative Stress Markers in the Aqueous Humor and Corneal Tissues of Patients With Congenital Hereditary Endothelial Dystrophy

Guha

Bhogapurapu

Ramappa

et al. 2020

Cornea

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Purpose: The aim of this study is to determine the presence of oxidative stress markers in the aqueous humor (AH) and corneal tissues of patients with congenital hereditary endothelial dystrophy (CHED). Methods: Interventional prospective study was undertaken to quantify levels of ascorbic acid and glutathione in the AH of patients with CHED. AH was collected from patients undergoing keratoplasty and levels of ascorbic acid and glutathione were determined using biochemical assays and measured spectrophotometrically. AH collected from pediatric patients with cataract were used as control. Corneal sections of patients who underwent penetrating keratoplasty were obtained, and presence of glutathione peroxidase 1, catalase, and superoxide dismutase was determined by immunohistochemistry. Tissue sections obtained from cadaveric corneas unsuitable for clinical transplant were used as control. Results: Significantly increased ascorbic acid levels were determined in patients with CHED (605.6 ± 158.9 μM) compared with those in controls (190.5 ± 74.72 μM). However, a trend toward reduced level of glutathione was detected in patients with CHED compared with that in the controls. Increased glutathione peroxidase 1 staining and reduced expression of catalase was detected in corneal tissues of patients with CHED compared with those in control corneal tissues. There was no apparent changes observed in the expression of superoxide dismutase in the corneal sections obtained from patients with CHED. Conclusions: To the best of our knowledge, this is the first study to determine the levels of ascorbic acid and glutathione in AH of patients with CHED. Our data suggest the presence of oxidative stress in CHED that might be responsible for the pathological changes in patients with CHED.

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Ascorbic acid and amino acid values in the aqueous humor of a patient with Lowe's syndrome

Abstract: In this infant with Lowe's syndrome, we found intracameral levels of amino acids similar to those in the plasma. Levels of ascorbic acid in the aqueous humor were decreased.

Cited by 3 publications

References 17 publications

Ascorbic acid concentrations in aqueous humor after systemic vitamin C supplementation in patients with cataract: pilot study

Ascorbic acid concentrations in aqueous humor after systemic vitamin C supplementation in patients with cataract: pilot study

Ascorbic acid concentration is reduced in the secondary aqueous humour of glaucomatous patients

Determination of Oxidative Stress Markers in the Aqueous Humor and Corneal Tissues of Patients With Congenital Hereditary Endothelial Dystrophy

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