Ascorbic Acid Alleviates Toxicity Induced by Excess Copper in <i>Brassica campestris</i> Ssp. <i>Chinensis</i> Makino

Zhou, Jihai; Ren, Jun; Wang, X. H.; Liu, T. K.; Hou, Xilin; Li, Y.

doi:10.1080/00103624.2017.1298777

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…The content of GSH and oxidized glutathione (GSSG) was measured according to the Solarbio reagent kit. The oxalic acid method was used for the determination of AsA levels in wucai leaves (Zhou et al., 2017). The reaction solution was used to assay AsA and dehydroascorbate (DHA) content by high‐performance liquid chromatography (Thermo Fisher Scientific).…”

Section: Methodsmentioning

confidence: 99%

The effect of exogenous 24‐epibrassinolide pretreatment on the quality, antioxidant capacity, and postharvest life of wucai (Brassica campestris L.)

Yuan

Nie

et al. 2021

Food Science & Nutrition

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The quality of green leafy vegetables is easily lost during the postharvest period. The effect of exogenous 24‐epibrassinolide (EBR) pretreatment on the quality of wucai was evaluated in the present study. Wucai plants were sprayed twice with 0.1 μM EBR before harvesting. Two storage temperatures were tested: 25°C and 4°C. At 4°C, EBR pretreatment significantly delayed the degradation of the pigment and plant water loss. Furthermore, we measured the activity of key enzymes of the ascorbic acid (AsA)‐glutathione (GSH) cycle, the content of the main metabolites, and the expression of the AsA metabolism‐related genes in leaves. The results indicated that all three plants showed stronger antioxidant capacity after EBR pretreatment. At 4°C and 25°C, the storage time of wucai was 20 days and 7 days after EBR treatment, while the samples could be stored for 14 days and 4 days without EBR treatment application, respectively. At 4°C, the nutritional properties of wucai pretreated with EBR, such as total free amino acids, total soluble sugar, and cellulose contents, were higher than those of the control, while the content of nitrite and lignin was lower than that of the control. Hence, EBR pretreatment was able to enhance the antioxidant capacity of wucai, maintain normal leaf color and shape during storage, and delay the decline of nutritional properties; therefore, EBR pretreatment has potential commercial value for prolonging the market life of wucai.

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Section: Methodsmentioning

confidence: 99%

The effect of exogenous 24‐epibrassinolide pretreatment on the quality, antioxidant capacity, and postharvest life of wucai (Brassica campestris L.)

Yuan

Nie

et al. 2021

Food Science & Nutrition

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“…It is documented that external supply of moderate ascorbic acid (≤50 mg L −1 ) mediates Mn toxicity in V. natans via antioxidant defence system, cytomembrane stability, soluble material, and photosynthetic pigment, but high dose of ascorbic acid (≥100 mg L −1 ) exacerbates Mn toxicity, although the pathway by which excess ascorbic acid inversely acts as a stress factor remains vague [54]. The extra addition of ascorbic acid at 1 mM alleviates Mn stress in hydroponic non-heading Chinese cabbage [55], and foliar spray of 5-10 µM ascorbic acid mitigates Mn toxicity in cowpea [56]. Further, transgenic technology has been adopted to increase foliar ascorbate levels as a strategy to adapt to multiple abiotic stresses, including Mn toxicity in rice [57].…”

Section: The Influence Of Ascorbic Acid Spraymentioning

confidence: 99%

The Combined Use of Soil Conditioner and Foliar Sulfur Spray Successfully Prevents Dark Pericarp Disease Induced by Manganese Toxicity in Litchi

Liu,

Bai,

Guo

et al. 2024

Agronomy

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Manganese toxicity is a major obstacle to agriculture in acid soils. Dark pericarp disease (DPD) is a newly spread physiological disorder induced by excess Mn in litchi, leading to undesirable fruit appearance and substantial economic loss. In this work, broadcast of alkaline soil conditioner in winter, followed by foliar sprays of ascorbic acid and sulfur solution at fruit development, was adopted to examine the effect of these combinations on DPD alleviation in a litchi orchard, with DPD morbidities of 70~85% in recent ten years. The combination of soil conditioner broadcast and foliar water spray was used as the control. At harvest, DPD incidence was significantly decreased by sulfur spray (3.3 ± 1.0%) and slightly reduced by ascorbic acid spray (10.7 ± 8.0%) compared to the control (12.9 ± 7.6%). Soil pH and available Mn were significantly increased and reduced by the soil conditioner broadcast. Sulfur spray significantly inhibited Mn uptake but enhanced the accumulation of Mg, Ca, sugars and cyanidin-3-rutinoside in the pericarp, leading to improved fruit pigmentation. Antioxidase activities were regulated to resist Mn stress by sulfur spray. The spray of ascorbic acid could not mitigate DPD as expected, probably due to the dose used. Conclusively, this study provides a practicable approach to mitigate Mn phytoavailability in acid soils.

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“…It implies a possible function related to DNA methylation and the epigenetic modulation of gene expression. Multiple plants differ in global methylation patterns elicited by excess copper and nickel [88,[91][92][93]. Different methylation patterns may also persist in later developmental stages to facilitate long-term adaptation to metal-contaminated sites [91].…”

Section: Deg Analysis Reveals Different Patterns Of Gene Expression B...mentioning

confidence: 99%

Decrypting Molecular Mechanisms Involved in Counteracting Copper and Nickel Toxicity in Jack Pine (Pinus banksiana) Based on Transcriptomic Analysis

Moy,

Nkongolo

2024

Plants

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The remediation of copper and nickel-afflicted sites is challenged by the different physiological effects imposed by each metal on a given plant system. Pinus banksiana is resilient against copper and nickel, providing an opportunity to build a valuable resource to investigate the responding gene expression toward each metal. The objectives of this study were to (1) extend the analysis of the Pinus banksiana transcriptome exposed to nickel and copper, (2) assess the differential gene expression in nickel-resistant compared to copper-resistant genotypes, and (3) identify mechanisms specific to each metal. The Illumina platform was used to sequence RNA that was extracted from seedlings treated with each of the metals. There were 449 differentially expressed genes (DEGs) between copper-resistant genotypes (RGs) and nickel-resistant genotypes (RGs) at a high stringency cut-off, indicating a distinct pattern of gene expression toward each metal. For biological processes, 19.8% of DEGs were associated with the DNA metabolic process, followed by the response to stress (13.15%) and the response to chemicals (8.59%). For metabolic function, 27.9% of DEGs were associated with nuclease activity, followed by nucleotide binding (27.64%) and kinase activity (10.16%). Overall, 21.49% of DEGs were localized to the plasma membrane, followed by the cytosol (16.26%) and chloroplast (12.43%). Annotation of the top upregulated genes in copper RG compared to nickel RG identified genes and mechanisms that were specific to copper and not to nickel. NtPDR, AtHIPP10, and YSL1 were identified as genes associated with copper resistance. Various genes related to cell wall metabolism were identified, and they included genes encoding for HCT, CslE6, MPG, and polygalacturonase. Annotation of the top downregulated genes in copper RG compared to nickel RG revealed genes and mechanisms that were specific to nickel and not copper. Various regulatory and signaling-related genes associated with the stress response were identified. They included UGT, TIFY, ACC, dirigent protein, peroxidase, and glyoxyalase I. Additional research is needed to determine the specific functions of signaling and stress response mechanisms in nickel-resistant plants.

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Ascorbic Acid Alleviates Toxicity Induced by Excess Copper in Brassica campestris Ssp. Chinensis Makino

Cited by 6 publications

References 29 publications

The effect of exogenous 24‐epibrassinolide pretreatment on the quality, antioxidant capacity, and postharvest life of wucai (Brassica campestris L.)

The effect of exogenous 24‐epibrassinolide pretreatment on the quality, antioxidant capacity, and postharvest life of wucai (Brassica campestris L.)

The Combined Use of Soil Conditioner and Foliar Sulfur Spray Successfully Prevents Dark Pericarp Disease Induced by Manganese Toxicity in Litchi

Decrypting Molecular Mechanisms Involved in Counteracting Copper and Nickel Toxicity in Jack Pine (Pinus banksiana) Based on Transcriptomic Analysis

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