Ascorbate radical: A valuable marker of oxidative stress

Buettner, Garry R.; Jurkiewicz, Beth Anne

doi:10.1007/978-3-0348-9074-8_11

Cited by 14 publications

(8 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conversely, the compound AAPH, being an initiator of alkylperoxyl radicals (ROO•), exerts the lowest catalytic action on the oxidation of the chicken breast meat dispersion system. Buettner and Jurkiewicz (1995) have suggested that the reduction potentials for the radicals are HO• Ͼ RO• Ͼ ROO•. The results obtained here for the rate of dissolved-oxygen consumption are in agreement with the reduction potential of these radicals except for hemoglobin.…”

Section: F Our Types Of Radical Initiation Systems Were Studiedsupporting

confidence: 88%

Effect of Antioxidants on the Oxidative Stability of Chicken Breast Meat in a Dispersion System

Lin¹,

Liang²

2002

Journal of Food Science

View full text Add to dashboard Cite

The antioxidant efficiency of ascorbic acid, a-tocopherol, Trolox C, and catechin were evaluated for chicken breast meat in a dispersion system at 37 °C. Peroxidation was induced by adding different kinds of initiators. The initiators exhibited the following order of catalytic action: ascorbic acid/Fe 21 . hemoglobin . Cu + /H 2 O 2 .2,2'-azobis (2-amidinopropane) hydrochloride (AAPH). The antioxidant efficiency of the antioxidants tested depended on their phase distribution and their reactivity to the radicals in various initiation systems. Among the antioxidants tested, catechin was the most effective antioxidant for chicken breast meat oxidation under the experimental conditions.

show abstract

Section: F Our Types Of Radical Initiation Systems Were Studiedsupporting

confidence: 88%

Effect of Antioxidants on the Oxidative Stability of Chicken Breast Meat in a Dispersion System

Lin¹,

Liang²

2002

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…The UV irradiation of murine or human skin generates the ascorbate radical, indicative of other radical precursors (1 1) and t-butylhydroperoxide has also been shown to induce free radical production in murine skin reflected by the formation of the ascorbate radical (12). The ESR detection of the ascorbate radical is accepted as a reliable marker of biological free radical production and oxidative stress (1 [3][4][5][6][7][8][9][10][11][12][13][14][15]. We have adapted both these methodologies to provide positive con-tAbbreviarions: DMPO, 5.5-dimethyl-1-pyrroline N-oxide; DMSO, dimethylsulfoxide; ESR, electron spin resonance; UVR, ultraviolet radiation.…”

Section: Introductionmentioning

confidence: 99%

Ruby Laser Irradiation (694 nm) of Human Skin Biopsies: Assessment by Electron Spin Resonance Spectroscopy of Free Radical Production and Oxidative Stress during Laser Depilation

Haywood

Wardman

Gault

et al. 1999

Photochem & Photobiology

View full text Add to dashboard Cite

Human skin biopsies (hair-bearing scalp skin and non-hair-bearing breast skin) were treated with t-butylhydroperoxide, irradiated with UV light (UVR) or irradiated with 694 nm ruby laser red light. Free-radical production and oxidative stress were assessed with electron spin resonance spectroscopy (ESR) using the ascorbate radical as a marker. In comparison with both UVR and t-butyl-hydroperoxide (which readily induce the ascorbate radical in hair-bearing and hairless skin), 694 nm red light does not result in the formation of the ascorbate radical in detectable concentrations. Spin-trapping experiments with the spin trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) showed that while free radicals could be detected after treatment of skin with t-butylhydroperoxide, radicals could not be trapped after laser treatment. Treatment of lasered skin (containing DMPO) with t-butylhydroperoxide produced radical adducts as well as the ascorbate radical, demonstrating that the laser neither depletes endogenous ascorbate nor the preadministered spin trap. It is concluded that 694 nm red light does not induce oxidative stress in human skin in levels comparable either to t-butyl hydroperoxide or UV light.

show abstract

“…Relatively less reactive ascorbate free radical (A •) ) is generated as a result. In our study however, the amount of ascorbyl free radical (A • H) that was generated in vitro from the L-ascorbic acid (present in vivo in tissues) was measured (Gonet 1994;Buettner & Jurkiewicz 1995). According to Roginsky and Stegmann (1994) the concentration of ascorbyl free radical is proportional to the tissue concentrations of L-ascorbic acid (reduced form only) and therefore may be used for indirect measurements of L-ascorbic acid concentrations.…”

mentioning

confidence: 99%

Increased Lipid Peroxidation and Ascorbic Acid Utilization in Testis and Epididymis of Rats Chronically Exposed to Lead

et al. 2006

View full text Add to dashboard Cite

The hypothesis has been recently presented that lead may exert its negative effect at least partially through the increase of reactive oxygen species (ROS) level in tissues. However, little is known about the influence of lead intoxication on equilibrium between generation and elimination of ROS in the male reproductive system. Sexually mature male Wistar rats were given ad libitum 1% of aqueous solution of lead acetate (PbAc) for 9 months. Significantly higher lead concentrations were found in blood [median 7.03 (Q25-Q75: 2.99-7.65) versus 0.18 (0.12-0.99) microg dl-1, P < 0.01], caput epididymis [median 5.51 (Q25-Q75: 4.31-7.83) versus 0.51 (0.11-0.80) microg g-1 d.m., P < 0.001], cauda epididymis [median 5.88 (Q25-Q75: 4.06-8.37) versus 0.61 (0.2 - 1.08) microg g-1 d.m., P < 0.001] and testis [median 1.81 (Q25-Q75: 0.94-2.31) versus 0.17 (0.03-0.3) microg g-1 d.m., P < 0.01] of lead-intoxicated rats when compared to the control. The concentration of ascorbyl radical, generated in vitro from L: -ascorbic acid (present in tissues in vivo) was measured by means of Electron Paramagnetic Resonance (EPR) spectroscopy. The EPR signal of ascorbyl radical in caput epididymis, cauda epididymis, testis and liver of lead acetate-treated animals revealed a significant decrease by 53%, 45%, 40% and 69% versus control tissues, respectively. Plasma L: -ascorbic acid content measured by high performance liquid chromatography (HPLC) method and total antioxidant status (TAS) measured by means of spectrophotometry were also significantly lower in the intoxicated versus control animals (28% and 21%, respectively). In the group exposed to lead the concentration of lipid peroxide in homogenates of the reproductive system organs was significantly elevated versus control group. It can be assumed that the lower EPR signal was caused by decreased tissue concentrations of L: -ascorbic acid. The latter may have resulted from consumption of ascorbic acid for scavenging of ROS excess in tissues of animals chronically exposed to lead.

show abstract

Ascorbate radical: A valuable marker of oxidative stress

Cited by 14 publications

References 74 publications

Effect of Antioxidants on the Oxidative Stability of Chicken Breast Meat in a Dispersion System

Effect of Antioxidants on the Oxidative Stability of Chicken Breast Meat in a Dispersion System

Ruby Laser Irradiation (694 nm) of Human Skin Biopsies: Assessment by Electron Spin Resonance Spectroscopy of Free Radical Production and Oxidative Stress during Laser Depilation

Increased Lipid Peroxidation and Ascorbic Acid Utilization in Testis and Epididymis of Rats Chronically Exposed to Lead

Contact Info

Product

Resources

About