Ascorbate in the leaf apoplast is a factor mediating ozone resistance in Plantago major

Zheng, Youbin; Lyons, Tom; Ollerenshaw, J. H.; Barnes, Jeremy

doi:10.1016/s0981-9428(00)00755-5

Cited by 59 publications

(35 citation statements)

References 38 publications

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“…Most studies showed stomata closure under stress, and consequent decrease in P N (Farquhar andSharkey 1982, Calatayud et al 2004). However, environmental stress may usually act on chloroplast directly with a decrease in P N , thus becoming non-stomata factors in restricting P N (Heath 1987, Mackerness 2000, Zheng et al 2000. Therefore, the factors causing a decrease in P N can be grouped into stomatal and non-stomatal ones (Shangguan et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Response of gas exchange and yield components of field-grown Triticum aestivum L. to elevated ozone in China

et al. 2007

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To assess photosynthesis and yield components' response of field-grown wheat to increasing ozone (O 3 ) concentration (based on diurnal pattern of ambient O 3 ) in China, winter wheat (Triticum aestivum L.) cv. Jia 403 was planted in open top chambers and exposed to three different O 3 concentrations: O 3 -free air (CF), ambient air (NF), and O 3 -free air with additional O 3 (CF+O 3 ). Diurnal changes of gas exchange and net photosynthetic rate (P N ) in response to photosynthetic photon flux density (PPFD) of flag leaves were measured at the filling grain stage, and yield components were investigated at harvest. High O 3 concentration altered diurnal course of gas exchange [P N , stomatal conductance (g s ), and intercellular CO 2 concentration (C i )] and decreased significantly their values except for C i . Apparent quantum yield (AQY), compensation irradiance (CI), and saturation irradiance (SI) were significantly decreased, suggesting photosynthetic capacity was also altered, characterized as reduced photon-saturated photosynthetic rate (P Nmax ). The limit of photosynthetic activity was probably dominated by non-stomatal factors in combination with stomatal closure. The significant reduction in yield was observed in CF+O 3 treatment as a result of a marked decrease in the ear length and the number of grains per ear, and a significant increase in the number of infertile florets per ear. Even though similar responses were also observed in plants exposed to ambient O 3 concentration, no statistical difference was observed at current ambient O 3 concentration in China.Additional key words: apparent quantum yield; diurnal pattern of O 3 ; net photosynthetic rate; irradiance; stomatal conductance; yield components.

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Section: Discussionmentioning

confidence: 99%

Response of gas exchange and yield components of field-grown Triticum aestivum L. to elevated ozone in China

et al. 2007

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“…The apoplastic ascorbate accounts for up to 5% of the whole leaf ascorbate pools (Noctor and Foyer, 1998) and appeared to be sufficient to detoxify a significant portion of O 3 under environmentally relevant conditions (Chameides, 1989;Lyons et al, 1999;Plochl et al, 2000;Frei et al, 2010). Also, most evidence has shown a close positive correlation between O 3 tolerance and apoplastic ascorbate contents in different plants (Burkey et al, 2003(Burkey et al, , 2006Feng et al, 2010;Frei et al, 2010;Zheng et al, 2000) with an exception in Trifolium repens (D'Haese et al, 2005). Therefore, the ascorbate especially in the apoplast has been used to represent the capacity of O 3 detoxification (Heath, 2008).…”

Section: Introductionmentioning

confidence: 99%

Diurnal variation of apoplastic ascorbate in winter wheat leaves in relation to ozone detoxification

Wang

Pang

Feng

et al. 2015

Environmental Pollution

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a b s t r a c tBesides stomatal closure, biological detoxification is an important protection mechanism for plants against ozone (O 3 ). This study investigated the diurnal changes of ascorbate (a major detoxification agent) in the apoplast and leaf tissues of winter wheat grown under ambient air field conditions. Results showed the reduced ascorbate in the apoplast (ASC apo ) exhibited a peak in late morning or midday, mismatching with either the maximum external O 3 concentrations in mid-afternoon or the maximum stomatal O 3 uptake between late morning and mid-afternoon. In contrast, the ASC in leaf tissues remained stable throughout the day. The investigations conducted in a Free-Air Concentration Elevation of O 3 system confirmed that the diurnal variations of the ASC apo were induced more by the daily variations of O 3 concentrations rather than the cumulative O 3 effects. In conclusion, the O 3 -stress detoxification should be a dynamic variable rather than a fixed threshold as assumed in the stomatal flux-based O 3 dose metrics.

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“…AsA is a powerful antioxidant that functions together with GSH and antioxidant enzymes to constitute the GSH-AsA cycle by which H 2 O 2 is detoxified. Several reports have shown the role of AsA in alleviating ozone toxicity by detoxifying ROS (Mächler et al, 1995;Moldau et al, 1998;Zheng et al, 2000). Recently, Maddison et al (2002) investigated the response of a sensitive radish genotype (Raphanus sativus L.) fed with L-Galactono-1, 4-lactone (GalL), the biosynthetic precursor of antioxidant ASA, to ozone toxicity.…”

Section: Discussionmentioning

confidence: 99%

“…Several reports have shown the role of AsA in alleviating ozone toxicity by detoxifying ROS (Mächler et al, 1995;Moldau et al, 1998;Zheng et al, 2000). There is evidence that L-Galactono-1, 4-lactone (GalL) is the biosynthetic precursor of the antioxidant AsA (Wheeler et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Cadmium‐induced oxidative stress and protection by L‐Galactono‐1, 4‐lactone in winter wheat (Triticum aestivum L.)

Zhao

Cai

Zhu

et al. 2005

Z. Pflanzenernähr. Bodenk.

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SummaryTwo hydroponic culture experiments were conducted to investigate cadmium (Cd)-induced oxidative stress in winter wheat (Triticum aestivum L.) seedlings and the effects of L-Galactono-1, 4-lactone (GalL), the biosynthetic precursor of the antioxidant ascorbate (AsA), on the oxidative stress induced by Cd. In experiment 1, with application of Cd (0, 10, 25, 50 lM) in nutrient solution, hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA) levels as well as membrane permeability in both shoots and roots were significantly increased, indicating Cd-induced oxidative stress and lipid peroxidation as well as plasma-membrane damage in the plants. In experiment 2, H 2 O 2 levels in plants exposed to Cd were significantly reduced by the addition of GalL (25 mM), associated with increased activities of peroxidase (POD), indicating that GalL alleviated the oxidative stress induced by Cd. Unexpectedly, however, the MDA levels were not reduced by the addition of GalL. Does Cd also induce lipid peroxidation directly besides via formation of reactive oxygen species (ROS)? This needs further study.

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Ascorbate in the leaf apoplast is a factor mediating ozone resistance in Plantago major

Cited by 59 publications

References 38 publications

Response of gas exchange and yield components of field-grown Triticum aestivum L. to elevated ozone in China

Response of gas exchange and yield components of field-grown Triticum aestivum L. to elevated ozone in China

Diurnal variation of apoplastic ascorbate in winter wheat leaves in relation to ozone detoxification

Cadmium‐induced oxidative stress and protection by L‐Galactono‐1, 4‐lactone in winter wheat (Triticum aestivum L.)

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