Ascorbate-Glutathione Cycle and Biotic Stress Tolerance in Plants

Kużniak, Elźbieta; Kopczewski, Tomasz; Chojak-Koźniewska, Joanna

doi:10.1007/978-3-319-74057-7_8

Cited by 14 publications

(14 citation statements)

References 165 publications

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“…It has been shown in several studies that microbial-mediated plant heat stress tolerance is associated with heightened antioxidant defense in inoculated plants. The contributions of the ascorbate-glutathione (AsA-GSH) cycle on plant tolerance under various abiotic stresses have been reported in many plant species ( Anjum et al, 2010 ; Bartoli et al, 2017 ; Kuźniak et al, 2017 ). The four enzymes that steer the cycle are ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), mono-dehydroascorbate reductase (MDHAR), and glutathione reductase (GR) ( Quan et al, 2008 ; Noctor et al, 2014 ; Mohi-Ud-Din et al, 2021 ).…”

Section: Meta-analytic Review and Discussion On Plant Tolerance To Th...mentioning

confidence: 99%

Microbe-Mediated Thermotolerance in Plants and Pertinent Mechanisms- A Meta-Analysis and Review

et al. 2022

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Microbial symbionts can mediate plant stress responses by enhancing thermal tolerance, but less attention has been paid to measuring these effects across plant-microbe studies. We performed a meta-analysis of published studies as well as discussed with relevant literature to determine how the symbionts influence plant responses under non-stressed versus thermal-stressed conditions. As compared to non-inoculated plants, inoculated plants had significantly higher biomass and photosynthesis under heat stress conditions. A significantly decreased accumulation of malondialdehyde (MDA) and hydrogen peroxide (H2O2) indicated a lower oxidation level in the colonized plants, which was also correlated with the higher activity of catalase, peroxidase, glutathione reductase enzymes due to microbial colonization under heat stress. However, the activity of superoxide dismutase, ascorbate oxidase, ascorbate peroxidase, and proline were variable. Our meta-analysis revealed that microbial colonization influenced plant growth and physiology, but their effects were more noticeable when their host plants were exposed to high-temperature stress than when they grew under ambient temperature conditions. We discussed the mechanisms of microbial conferred plant thermotolerance, including at the molecular level based on the available literature. Further, we highlighted and proposed future directions toward exploring the effects of symbionts on the heat tolerances of plants for their implications in sustainable agricultural production.

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Section: Meta-analytic Review and Discussion On Plant Tolerance To Th...mentioning

confidence: 99%

Microbe-Mediated Thermotolerance in Plants and Pertinent Mechanisms- A Meta-Analysis and Review

et al. 2022

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“…Among ROS, H 2 O 2 which is a relatively stable and non-charged molecule, is considered a versatile signaling molecule involved in the intracellular communication system that activates stress responses, including redox-dependent reprogramming of defense gene expression [8,9]. The ROS-processing systems such as the antioxidant enzymes, the ascorbate-glutathione cycle and tocopherols, have been shown to play an important role in mAnaging ROS, particularly H 2 O 2 , generated at the sites of infections and in initiating signals eliciting downstream defense reactions at the local and systemic levels [10,11]. The ascorbate-glutathione cycle is an antioxidant system, but it also fulfils regulatory functions in redox signaling.…”

Section: Introductionmentioning

confidence: 99%

“…The ROS-processing systems such as the antioxidant enzymes, the ascorbate–glutathione cycle and tocopherols, have been shown to play an important role in managing ROS, particularly H 2 O 2 , generated at the sites of infections and in initiating signals eliciting downstream defense reactions at the local and systemic levels [ 10 , 11 ]. The ascorbate–glutathione cycle is an antioxidant system, but it also fulfils regulatory functions in redox signaling.…”

Section: Introductionmentioning

confidence: 99%

Local and Systemic Changes in Photosynthetic Parameters and Antioxidant Activity in Cucumber Challenged with Pseudomonas syringae pv lachrymans

Kopczewski

Kużniak

Kornaś

et al. 2020

IJMS

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We studied changes in gas exchange, photochemical activity and the antioxidant system in cucumber leaves locally infected with Pseudomonas syringae pv lachrymans and in uninfected systemic ones. Infection-induced declined net photosynthesis rate and the related changes in transpiration rate, the intracellular CO2 concentration, and prolonged reduction in maximal PSII quantum yield (Fv/Fm), accompanied by an increase in non-photochemical quenching (NPQ), were observed only in the infected leaves, along with full disease symptom development. Infection severely affected the ROS/redox homeostasis at the cellular level and in chloroplasts. Superoxide dismutase, ascorbate, and tocopherol were preferentially induced at the early stage of pathogenesis, whereas catalase, glutathione, and the ascorbate–glutathione cycle enzymes were activated later. Systemic leaves retained their net photosynthesis rate and the changes in the antioxidant system were partly like those in the infected leaves, although they occurred later and were less intense. Re-balancing of ascorbate and glutathione in systemic leaves generated a specific redox signature in chloroplasts. We suggest that it could be a regulatory element playing a role in integrating photosynthesis and redox regulation of stress, aimed at increasing the defense capacity and maintaining the growth of the infected plant.

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“…The AsA-GSH cycle is a crucial element of the ROS homeostasis mechanism in plants [ 87 ], and it also plays contributes vital role in adjusting plant-pathogen interactions. Recently, special attention has been paid to explore the regulatory functions of the AsA–GSH cycle in plant defense against biotic stress [ 88 ]. As an antioxidant, GSH is essential for plant signal transduction during biotic stress [ 89 ].…”

Section: Discussionmentioning

confidence: 99%

Dissection of Paenibacillus polymyxa NSY50-Induced Defense in Cucumber Roots against Fusarium oxysporum f. sp. cucumerinum by Target Metabolite Profiling

Yang

Guo

et al. 2022

Biology

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To gain insights into the roles of beneficial PGPR in controlling soil-borne disease, we adopted a metabolomics approach to investigate the beneficial impacts of P. polymyxa NSY50 on cucumber seedling roots under the pathogen of Fusarium oxysporum f. sp. cucumerinum (FOC). We found that NSY50 pretreatment (NSY50 + FOC) obviously reduced the production of reactive oxygen species (ROS). Untargeted metabolomic analysis revealed that 106 metabolites responded to NSY50 and/or FOC inoculation. Under FOC stress, the contents of root osmotic adjustment substances, such as proline and betaine were significantly increased, and dehydroascorbic acid and oxidized glutathione (GSH) considerably accumulated. Furthermore, the contents of free amino acids such as tryptophan, phenylalanine, and glutamic acid were also significantly accumulated under FOC stress. Similarly, FOC stress adversely affected glycolysis and the tricarboxylic acid cycles and transferred to the pentose phosphate pathway. Conversely, NSY50 + FOC better promoted the accumulation of α-ketoglutaric acid, ribulose-5-phosphate, and 7-phosphosodiheptanone compared to FOC alone. Furthermore, NSY50 + FOC activated GSH metabolism and increased GSH synthesis and metabolism-related enzyme activity and their encoding gene expressions, which may have improved redox homoeostasis, energy flow, and defense ability. Our results provide a novel perspective to understanding the function of P. polymyxa NSY50, accelerating the application of this beneficial PGPR in sustainable agricultural practices.

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Ascorbate-Glutathione Cycle and Biotic Stress Tolerance in Plants

Cited by 14 publications

References 165 publications

Microbe-Mediated Thermotolerance in Plants and Pertinent Mechanisms- A Meta-Analysis and Review

Microbe-Mediated Thermotolerance in Plants and Pertinent Mechanisms- A Meta-Analysis and Review

Local and Systemic Changes in Photosynthetic Parameters and Antioxidant Activity in Cucumber Challenged with Pseudomonas syringae pv lachrymans

Dissection of Paenibacillus polymyxa NSY50-Induced Defense in Cucumber Roots against Fusarium oxysporum f. sp. cucumerinum by Target Metabolite Profiling

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