Ascorbate Free Radical Stimulates Onion Root Growth by Increasing Cell Elongation

Hidalgo, Antonio; Garcı́a-Herdugo, G.; González-Reyes, José A.; Morré, D. James; Navas, Plácido

doi:10.1086/337891

Cited by 48 publications

(27 citation statements)

References 28 publications

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“…The results reported in this paper show that previously published data concerning the stimulation of root growth and cell elongation by AFR, but not by ASC or DHA (Hidalgo et al, 1989(Hidalgo et al, , 1991, can be correlated with a significant increase in the uptake of nitrate and sugars. The previous findings demonstrated that long-tem (48 h) treatments with AFR led to a more rapid rate of onion root growth (Hidalgo et al, 1991) because of a significant stimulation of cell elongation.…”

Section: Discussionsupporting

confidence: 70%

“…Uptake and assimilation of nitrate by vacuoles have been studied (Beevers and Hageman, 1983;Reinhold and Kaplan, 1984), and it has been proposed that nitrate is transported into the vacuole in response to an interior positive membrane potential set up by the action of the tonoplast H+-ATPase Taiz, 1985a, 1985b;Kaestner and Sze, 1987). The increase in nitrate assimilation studied here can be correlated with an increased tonoplast ATPase activity after 48 h of treatment with AFR (Hidalgo et al, 1991). Both phenomena appear to be related to increased tonoplast synthesis in AFR-treated roots.…”

Section: Discussionmentioning

confidence: 67%

“…The previous findings demonstrated that long-tem (48 h) treatments with AFR led to a more rapid rate of onion root growth (Hidalgo et al, 1991) because of a significant stimulation of cell elongation. There is a higher vacuolization in AFR-stimulated roots (Hidalgo et al, 1989), which constitutes an essential step in the cell enlargement process.…”

Section: Discussionmentioning

confidence: 93%

“…6B). Because vacuole volume and tonoplast surface area grow significantly during 48 h of AFR pretreatment (Hidalgo et al, 1991), we investigated the changes in these vacuolar parameters during the AFR, ASC, and DHA incubations. Both vacuole Vv and tonoplast Sv increased gradually during the AFR pretreatment, exhibiting kinetics very similar to those observed for nitrate and Suc uptake.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, we showed that AFR stimulates root growth in Allium cepa by increasing elongation without affecting cell division or proliferation, as a result of an augmented relative volume occupied by vacuoles (cell vacuolization; Hidalgo et al, 1989Hidalgo et al, , 1991. These findings suggest a linkage between the redox chain and processes related to cell growth.…”

mentioning

confidence: 96%

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Nutrient Uptake Changes in Ascorbate Free Radical-Stimulated Onion Roots

González‐Reyes¹,

Hidalgo²,

Caler³

et al. 1994

Plant Physiol.

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Long-term treatments with ascorbate free radical-stimulated glucose, fucose, sucrose, and nitrate uptake in Allium cepa roots.Clucose and fucose showed saturation kinetics in untreated roots, but after treatment with the ascorbate free radical, uptake was linear with time. Although the rates of nitrate and sucrose uptake increased after treatment with ascorbate free radical, the kinetics were similar to those observed in the controls. Ascorbate and dehydroascorbate inhibited nutrient uptake. l h e uptake rates for all nutrients increased throughout the 48-h period of pretreatment with ascorbate free radical. During the treatment an increase in the vacuole volume and tonoplast surface area also occurred. These results show the relationship between an increase in vacuolar volume and stimulated nutrient uptake from ascorbate-free radical, resulting in enhanced root elongation. These results suggest that activation of a transplasma membrane redox system by ascorbatefree radical is involved in these responses.

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Section: Discussionsupporting

confidence: 70%