Ascorbate concentration in<i>Arabidopsis thaliana</i>and expression of ascorbate related genes using RNAseq in response to light and the diurnal cycle

Laing, William A.; Norling, Cara; Brewster, D. T.; Wright, Michele; Bulley, Sean

doi:10.1101/138008

Cited by 11 publications

(20 citation statements)

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“…Therefore, conclusions on the correlation between the gene expression, activity of the corresponding enzymes of the pathway, and the ascorbate pool size are inconsistent between studies. That being said, the comparison of multiple light-shift experiments revealed GDP-L-galactose phosphorylase (GGP) as the key enzyme of the pathway controlling the ascorbate levels under high light [54,[56][57][58][59]. The corresponding genes encoding this enzyme, the first committed step of the ascorbate biosynthetic pathway, are VTC2 and VTC5 paralogs, which were identified to be induced in concert upon 24 h exposure to high light, leading to a 20-fold increase in the activity of the corresponding enzyme, and an increase in ascorbate levels [56].…”

Section: Light Regulation Of Ascorbatementioning

confidence: 99%

“…In a study where the authors explored the transcriptional regulation of ascorbate by RNA-seq following a step change of light intensity in Arabidopsis, VTC2 and, to lesser extent, VTC5 were validated as regulatory points in light accumulation of ascorbate, the expression of both genes were correlated with different light intensities, however, a minor change in GDP mannose pyrophosphorylase (GMP) could also be observed [57]. Moreover, GGP has been proposed as a key rate-limiting step for ascorbate biosynthesis not only in Arabidopsis [60], but also in other species including tobacco [61], apple [62], and kiwifruit [60,63].…”

Section: Light Regulation Of Ascorbatementioning

confidence: 99%

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Ascorbate and Thiamin: Metabolic Modulators in Plant Acclimation Responses

Rosado-Souza

Fernie

Aarabi

2020

Plants

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Cell compartmentalization allows incompatible chemical reactions and localised responses to occur simultaneously, however, it also requires a complex system of communication between compartments in order to maintain the functionality of vital processes. It is clear that multiple such signals must exist, yet little is known about the identity of the key players orchestrating these interactions or about the role in the coordination of other processes. Mitochondria and chloroplasts have a considerable number of metabolites in common and are interdependent at multiple levels. Therefore, metabolites represent strong candidates as communicators between these organelles. In this context, vitamins and similar small molecules emerge as possible linkers to mediate metabolic crosstalk between compartments. This review focuses on two vitamins as potential metabolic signals within the plant cell, vitamin C (L-ascorbate) and vitamin B1 (thiamin). These two vitamins demonstrate the importance of metabolites in shaping cellular processes working as metabolic signals during acclimation processes. Inferences based on the combined studies of environment, genotype, and metabolite, in order to unravel signaling functions, are also highlighted.

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Section: Light Regulation Of Ascorbatementioning

confidence: 99%

Section: Light Regulation Of Ascorbatementioning

confidence: 99%

Ascorbate and Thiamin: Metabolic Modulators in Plant Acclimation Responses

Rosado-Souza

Fernie

Aarabi

2020

Plants

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“…Various abiotic inputs such as light and stresses such as salt, UV, ozone, metals, nitric oxide, temperature extremes and drought are also known to influence AsA metabolism [25,34,[54][55][56][57][58][59][60][61][62][63][64][65][66][67][68][69][70]. Light is an elicitor of AsA metabolism and AsA undergoes diurnal change in leaves [30,47,69,71] although Laing et al [72] did not observe diurnal changes in ascorbate in Arabidopsis, even though genes for GGP and GME showed strong diurnal trends. That study used mature plants, not seedlings and did report long-term responses to growth in response to differing photon flux densities, but not short-term changes.…”

Section: Introductionmentioning

confidence: 98%

Elevating Ascorbate in Arabidopsis Stimulates the Production of Abscisic Acid, Phaseic Acid, and to a Lesser Extent Auxin (IAA) and Jasmonates, Resulting in Increased Expression of DHAR1 and Multiple Transcription Factors Associated with Abiotic Stress Tolerance

Bulley

Cooney

Laing

2021

IJMS

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Gene expression and phytohormone contents were measured in response to elevating ascorbate in the absence of other confounding stimuli such as high light and abiotic stresses. Young Arabidopsis plants were treated with 25 mM solutions of l-galactose pathway intermediates l-galactose (l-gal) or l-galactono-1,4-lactone (l-galL), as well as L-ascorbic acid (AsA), with 25 mM glucose used as control. Feeding increased rosette AsA 2- to 4-fold but there was little change in AsA biosynthetic gene transcripts. Of the ascorbate recycling genes, only Dehydroascorbate reductase 1 expression was increased. Some known regulatory genes displayed increased expression and included ANAC019, ANAC072, ATHB12, ZAT10 and ZAT12. Investigation of the ANAC019/ANAC072/ATHB12 gene regulatory network revealed a high proportion of ABA regulated genes. Measurement of a subset of jasmonate, ABA, auxin (IAA) and salicylic acid compounds revealed consistent increases in ABA (up to 4.2-fold) and phaseic acid (PA; up to 5-fold), and less consistently certain jasmonates, IAA, but no change in salicylic acid levels. Increased ABA is likely due to increased transcripts for the ABA biosynthetic gene NCED3. There were also smaller increases in transcripts for transcription factors ATHB7, ERD1, and ABF3. These results provide insights into how increasing AsA content can mediate increased abiotic stress tolerance.

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“…The level of Asc depends on the developmental stage, diurnal rhythm, light, and many external factors that introduce stress [44]. Diurnal variation in the transcript levels of genes involved in AsA synthesis was reported in tomato [48] and Arabidopsis [49]. Apart from the definite involvement of Asc in the regulation of whole-plant development [50], Asc modulates the growth of woody plants in further manners.…”

Section: The Involvement Of Asc In the Development Of Plantsmentioning

confidence: 99%

Ascorbic Acid—The Little-Known Antioxidant in Woody Plants

et al. 2019

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Reactive oxygen species (ROS) are constantly produced by metabolically active plant cells. The concentration of ROS may determine their role, e.g., they may participate in signal transduction or cause oxidative damage to various cellular components. To ensure cellular homeostasis and minimize the negative effects of excess ROS, plant cells have evolved a complex antioxidant system, which includes ascorbic acid (AsA). AsA is a multifunctional metabolite with strong reducing properties that allows the neutralization of ROS and the reduction of molecules oxidized by ROS in cooperation with glutathione in the Foyer-Halliwell-Asada cycle. Antioxidant enzymes involved in AsA oxidation and reduction switches evolved uniquely in plants. Most experiments concerning the role of AsA have been performed on herbaceous plants. In addition to extending our understanding of this role in additional taxa, fundamental knowledge of the complex life cycle stages of woody plants, including their development and response to environmental factors, will enhance their breeding and amend their protection. Thus, the role of AsA in woody plants compared to that in nonwoody plants is the focus of this paper. The role of AsA in woody plants has been studied for nearly 20 years. Studies have demonstrated that AsA is important for the growth and development of woody plants. Substantial changes in AsA levels, as well as reduction and oxidation switches, have been reported in various physiological processes and transitions described mainly in leaves, fruits, buds, and seeds. Evidently, AsA exhibits a dual role in the photoprotection of the photosynthetic apparatus in woody plants, which are the most important scavengers of ozone. AsA is associated with proper seed production and, thus, woody plant reproduction. Similarly, an important function of AsA is described under drought, salinity, temperature, light stress, and biotic stress. This report emphasizes the involvement of AsA in the ecological advantages, such as nutrition recycling due to leaf senescence, of trees and shrubs compared to nonwoody plants.

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Ascorbate concentration inArabidopsis thalianaand expression of ascorbate related genes using RNAseq in response to light and the diurnal cycle

Abstract: 25We explore where transcriptional regulation of ascorbate concentration lies in plants. Is were harvested at the four PFD levels and over a diurnal cycle and after a step change in PFD 29 and analysed for ascorbate concentration and transcript levels measured by RNAseq.

Cited by 11 publications

References 57 publications

Ascorbate and Thiamin: Metabolic Modulators in Plant Acclimation Responses

Ascorbate and Thiamin: Metabolic Modulators in Plant Acclimation Responses

Elevating Ascorbate in Arabidopsis Stimulates the Production of Abscisic Acid, Phaseic Acid, and to a Lesser Extent Auxin (IAA) and Jasmonates, Resulting in Increased Expression of DHAR1 and Multiple Transcription Factors Associated with Abiotic Stress Tolerance

Ascorbic Acid—The Little-Known Antioxidant in Woody Plants

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