Ascorbate Biosynthesis in Arabidopsis Cell Suspension Culture

Davey, Mark W.; Gilot, Christophe; Persiau, Geert; Østergaard, Jens; Han, Yu; Bauw, Guy; Montagu, Marc C. Van

doi:10.1104/pp.121.2.535

Cited by 163 publications

(143 citation statements)

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“…The biosynthesis of AsA proceeds via L-Gal (Wheeler et al, 1998), although conclusive evidence for all steps has only relatively recently become available (Conklin et al, 2006;Laing et al, 2007). Alternative biosynthetic routes involving uronic acids (Davey et al, 1999;Agius et al, 2003), L-gulose (Wolucka and Van Montagu, 2003), or myoinositol (Lorence et al, 2004) have been proposed in several plant species, including apple Razavi et al, 2005; Fig. 1), but their physiological relevance and contribution to the AsA pool is still far from clear in most plant species, with the possible exception of strawberry (Fragaria 3 ananassa; Agius et al, 2003;Cruz-Rus et al, 2011;Zorrilla-Fontanesi et al, 2011).…”

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confidence: 99%

Allelic Variation in Paralogs of GDP-l-Galactose Phosphorylase Is a Major Determinant of Vitamin C Concentrations in Apple Fruit

et al. 2012

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To identify the genetic factors underlying the regulation of fruit vitamin C (L-ascorbic acid [AsA]) concentrations, quantitative trait loci (QTL) studies were carried out in an F1 progeny derived from a cross between the apple (Malus 3 domestica) cultivars Telamon and Braeburn over three years. QTL were identified for AsA, glutathione, total antioxidant activity in both flesh and skin tissues, and various quality traits, including flesh browning. Four regions on chromosomes 10, 11, 16, and 17 contained stable fruit AsA-QTL clusters. Mapping of AsA metabolic genes identified colocations between orthologs of GDP-L-galactose phosphorylase (GGP), dehydroascorbate reductase (DHAR), and nucleobase-ascorbate transporter within these QTL clusters. Of particular interest are the three paralogs of MdGGP, which all colocated within AsA-QTL clusters. Allelic variants of MdGGP1 and MdGGP3 derived from the cultivar Braeburn parent were also consistently associated with higher fruit total AsA concentrations both within the mapping population (up to 10-fold) and across a range of commercial apple germplasm (up to 6-fold). Striking differences in the expression of the cv Braeburn MdGGP1 allele between fruit from high-and low-AsA genotypes clearly indicate a key role for MdGGP1 in the regulation of fruit AsA concentrations, and this MdGGP allele-specific single-nucleotide polymorphism marker represents an excellent candidate for directed breeding for enhanced fruit AsA concentrations. Interestingly, colocations were also found between MdDHAR3-3 and a stable QTL for browning in the cv Telamon parent, highlighting links between the redox status of the AsA pool and susceptibility to flesh browning.

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Allelic Variation in Paralogs of GDP-l-Galactose Phosphorylase Is a Major Determinant of Vitamin C Concentrations in Apple Fruit

et al. 2012

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“…O acréscimo nos teores de ácido ascórbico dos frutos, durante o desenvolvimento, deve-se ao aumento paralelo da glicose nos tecidos, molécula precursora da vitamina C (Lehninger et al, 2002;Davey et al, 1999). Tais aspectos podem ser observados até o ponto em que os frutos atingem a maturidade fisiológica (Chitarra & Chitarra, 2005), pois estes valores podem oscilar ou mesmo decair durante as fases pós-colheita (Awad, 1993).…”

Section: Resultsunclassified

Caracterização física e química de melão durante o seu desenvolvimento

et al. 2007

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RESUMO -O objetivo deste trabalho foi estudar algumas alterações físicas e químicas durante o desenvolvimento de frutos de melão de quatro cultivares. Para isto foram plantadas quatro cultivares de melão na área experimental do Departamento de Ciências Vegetais da Universidade Federal Rural do Semi-Árido -UFERSA, onde foram monitorados os períodos da antese. O delineamento experimental foi em blocos completos casualizados, com três repetições, em esquema de parcela subdividida. As parcelas foram constituídas pelas cultivares de melão ('AF 1749', Hy-Mark, Rochedo e Caipira) e as subparcelas pelas idades dos frutos (14; 21; 28; 35 e 42 dias após a antese). Quatro frutos por subparcela foram colhidos nestas idades e foram analisados quanto à massa média, firmeza, sólidos solúveis, acidez total, vitamina C e pH. Verificou-se interação significativa entre os fatores estudados (idades x cultivares) para massa média dos frutos, firmeza de polpa, acidez total, pH, vitamina C e sólidos solúveis. A massa dos frutos, firmeza, sólidos solúveis, pH e Vitamina C aumentaram, e a acidez diminuiu durante o desenvolvimento dos frutos. Termos para Indexação: Cucumis melo L., qualidade de frutos, idades dos frutos, antese. PHYSICAL AND CHEMICAL CHARACTERIZATION OF MELON FRUITS DURING THEIR DEVELOPMENTABSTRACT -The objective of this work was to study some physical and chemical changes during melon fruits development of four cultivars. An experiment was carried out at the experimental area of Plant Science Department of Universidade Federal Rural do SemiÁrido -UFERSA, where the anthesis period was monitored. The experimental design used was of randomized complete blocks with three replications in a split-plot scheme. To the plots were assigned the melon cultivars ('AF 1749', Hy-Mark, Rochedo e Caipira) and to the subplots were assigned the harvested fruit times (7, 14, 21, 28, 35 and 42 days after anthesis). Four fruits per subplot of each cultivar were harvested in the different fruit times, and then, they were analyzed for average weight, firmness, soluble solids, total acidity, vitamin C and pH. There was a significant interaction between harvested fruit times and cultivars for average fruit weight, pulp firmness, total acidity, pH, vitamin C and soluble solids. The average fruit weight, pulp firmness, soluble solids, pH and Vitamin C increased and the total acidity decreased with the melon fruit's development.

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“…This lactone then spontaneously isomerises to l-ascorbic acid (Wheeler et al 1998;Davey et al 1999;Loewus 1999;Smirnoff 2001). …”

Section: Biosynthesis In Plantsmentioning

confidence: 99%

Biosynthesis of food constituents: Vitamins. 2. Water-soluble vitamins: Part 2 - a review

Velı́šek¹,

Cejpek²

2007

Czech J. Food Sci.

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This review article gives a survey of the biosynthetic pathways that lead to water-soluble vitamins in microorganisms, plants and some animals. The biosynthetic pathways leading to some the B-group vitamins (biotin, folacin, cobalamins) and to vitamin C are described in detail using reaction schemes and mechanisms with enzymes involved and detailed explanations based on chemical principles and mechanisms.

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Ascorbate Biosynthesis in Arabidopsis Cell Suspension Culture

Cited by 163 publications

References 47 publications

Allelic Variation in Paralogs of GDP-l-Galactose Phosphorylase Is a Major Determinant of Vitamin C Concentrations in Apple Fruit

Allelic Variation in Paralogs of GDP-l-Galactose Phosphorylase Is a Major Determinant of Vitamin C Concentrations in Apple Fruit

Caracterização física e química de melão durante o seu desenvolvimento

Biosynthesis of food constituents: Vitamins. 2. Water-soluble vitamins: Part 2 - a review

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