Ascorbate and glutathione: guardians of the cell cycle, partners in crime?

Potters, Geert; Gara, Laura De; Asard, Han; Horemans, Nele

doi:10.1016/s0981-9428(02)01414-6

Cited by 252 publications

(164 citation statements)

References 91 publications

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“…This would explain why applications of BSO reduce the overall size of MDEs (Belmonte et al 2006). In agreement with previous studies, which show a correlation between ascorbate levels and cell division and elongation (reviewed by Potters et al 2005), low ascorbate levels in BSO-treated MDEs also correlate with a reduction in cell elongation, although no eVect on cell division was observed (data not shown). During the late phases of development the BSO-induced oxidized glutathione environment aVects the transcription and activity of MDHAR, as well as the composition of the total ascorbate pool, which becomes enriched with the oxidized forms, i.e.…”

Section: Antioxidant Responsesupporting

confidence: 93%

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Buthionine sulfoximine (BSO)-mediated improvement in cultured embryo quality in vitro entails changes in ascorbate metabolism, meristem development and embryo maturation

Stasolla

Belmonte

Tahir

et al. 2008

Planta

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Applications of buthionine sulfoximine (BSO), an inhibitor of GSH (reduced glutathione), which switches the cellular glutathione pool towards the oxidized form GSSG, positively inXuences embryo quality by improving the structure of the shoot apical meristem and promoting embryo maturation, both of which improve the post-embryonic performance of the embryos. To investigate the mechanisms underlying BSO-mediated improvement in embryo quality the transcript proWles of developing Brassica napus microspore-derived embryos cultured in the absence (control) or presence of BSO were analyzed using a 15,000-element B. napus oligo microarray. BSO applications induced major changes in transcript accumulation patterns, especially during the late phases of embryogenesis. BSO aVected the transcription and activities of key enzymes involved in ascorbate metabolism, which resulted in major Xuctuations in cellular ascorbate levels. These changes were related to morphological characteristics of the embryos and their postembryonic performance. BSO applications also activated many genes controlling meristem formation and function, including ZWILLE, SHOOTMERISTEMLESS, and ARG-ONAUTE 1. Increased expression of these genes may contribute to the improved structural quality of the shoot poles observed in the presence of BSO. Compared to their control counterparts, middle-and late-stage BSO-treated embryos also showed increased accumulation of transcripts associated with the maturation phase of zygotic embryo development, including genes encoding ABA-responsive proteins and storage-and late-embryogenic abundant (LEA) proteins. Overall these transcriptional changes support the observation that the BSO-induced oxidized glutathione redox state allows cultured embryos to reach both morphological and physiological maturity, which in turn guarantees successful regeneration and enhanced post-embryonic growth.

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Section: Antioxidant Responsesupporting

confidence: 93%

“…Buthionine sulfoximine altered APX activity and the cellular ascorbate pool, both of which are implicated in cell wall loosening (reviewed by Potters et al 2005). We therefore hypothesized that cell elongation was aVected in BSOtreated embryos.…”

Section: Cell Measurementsmentioning

confidence: 99%

Buthionine sulfoximine (BSO)-mediated improvement in cultured embryo quality in vitro entails changes in ascorbate metabolism, meristem development and embryo maturation

Stasolla

Belmonte

Tahir

et al. 2008

Planta

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“…3,4 However, the processes affected by P-deficiency, namely, cell division and elongation are to a large extent, regulated by redox factors like radical oxygen species (ROS) or ascorbate. 7 Redox agents are involved in an auxindependent patterning in the root apical meristem. Cells of the quiescent centre (QC) that accumulate high auxin levels are characterized by the oxidized status of ascorbate and glutathione and the overproduction of ROS.…”

Section: Defines the Patterns Of Root Architecture In Arabidopsis Affmentioning

confidence: 99%

A role for redox factors in shaping root architecture under Phosphorus deficiency

Tyburski

Dunajska

Tretyn

2010

Plant Signaling & Behavior

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Addendum to: Tyburski J, Dunajska K, Tretyn A. Reactive oxygen species localization in roots of Arabidopsis thaliana seedlings under phosphate deficiency. Plant Growth Regul 2009; 59:27-36; DOI: 10.1007/s10725-009-9385-9. T he developmental response of the Arabidopsis root system to low phosphorus (P) availability involves the reduction in primary root elongation accompanied by the formation of numerous lateral roots. We studied the roles of selected redox metabolites, namely, radical oxygen species (ROS) and ascorbic acid (ASC) in the regulation of root system architecture by different P availability. Rapidly growing roots of plants grown on P-sufficient medium synthesize ROS in root elongation zone and quiescent centre. We have demonstrated that the arrest of root elongation at low P medium coincides with the disappearance of ROS from the elongation zone. P-starvation resulted in a decrease in ascorbic acid level in roots. This correlated with a decrease in cell division activity. On the other hand, feeding P-deficient plants with ASC, stimulated mitotic activity in the primary root meristem and partly reversed the inhibition of root growth imposed by low P conditions. In this paper, we discuss the idea of the involvement of redox agents in the regulation of root system architecture under low P availability. Phosphorus (P) Availability Defines the Patterns of Root Architecture in Arabidopsis Affecting Cell Divisions and ElongationThe seedlings of Arabidopsis thaliana develop clearly distinguishable patterns of root system architecture in response to variable P availability. Cultures at low P concentration (1 μM) result in a reduction in primary root growth, increased lateral root formation and enhanced root hair development. On the other hand, at high P concentration (1 mM), the root system is composed of a long primary root with few lateral roots and short root hairs. 1-3Responses of the root system to P deficiency are dependent on changes in cell proliferation. Arrest of primary root growth at low P availability is due to the inhibition of cell division and the onset of cell differentiation within the primary root meristem. Mitotic activity is relocated to the sites of lateral root formation, which results in an increased lateral root density. In a manner similar to the primary root tip, cell differentiation in older lateral roots occurs within the apical root meristem, which is followed by an arrest in lateral root elongation. 4,5 Besides the reduction in cell division rate, low P treatments inhibit cell growth in root elongation zone. 5,6 Promotion of lateral root development and the arrest of cell divisions in the apices of roots of P-starved plants results from changes in auxin transport and/or sensitivity.3,4 However, the processes affected by P-deficiency, namely, cell division and elongation are to a large extent, regulated by redox factors like radical oxygen species (ROS) or ascorbate. 7Redox agents are involved in an auxindependent patterning in the root apical meristem. Cells of the quiescent ...

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“…some researches identified morphological and biochemichal molecular markers giving a general phisiological condition of the cuttings with relation to rooting capability, and useful for application purposes (Fernandez-Lorenzo et al, 1999;De Klerk et al, 1999). cellular redox status is considered an important regulator of cellular metabolism (sànchez-Fernàndez et al, 1997;Potters et al, 2002). cytoplasmic equilibrium between the reduced and oxidized forms of ascorbic acid (asc and Dha) and glutathione (Gsh and GssG) is considered a main factor influencing cellular redox homeostasis.…”

Section: Introductionmentioning

confidence: 99%

“…ascorbic acid and glutathione are known to be involved in the process of detoxification of ros (reactive oxygen species) and more recently in processes related to plant growth regulation at levels of cell. division and elongation (Potters et al, 2002), typical events in new roots formation.…”

Section: Introductionmentioning

confidence: 99%

Extraction and determination of ascorbate and dehydroascorbate from apoplastic fluid of stem of rooted and non-rooted cuttings in relation to the rhizogenesis

Zuffellato-Ribas

Morini

Picciarelli

et al. 2010

Braz. J. Plant Physiol.

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abstractstarting from semihardwood branches of peach and plum rootstocks (Prunus cerasifera E.) cv. mrs2/5, plum rootstocks (Prunus cerasifera × P. salicina) × (P. cerasifera × P. persica) cv. ishtarà and olive tree (Olea europaea L.) cv. Frantoio, cuttings were collected and treated with 0 mg.L -1 and 2500 mg.L -1 of indolebutyric acid (iBa) by immersion in the base of the cuttings. after 60 days of planting, the cultivars mrs2/5, ishtarà and Frantoio presented a significative difference with relation to the rooting induction by application of the indolebutyric acid (27.5, 47.5 and 41.25 %, respectively), inducing a higher number of roots per cutting without at the same time significantly increasing the length of the roots themselves. the levels of ascorbate and dehydroascorbate in the rooted and non-rooted cuttings of the three cultivars were determined. these parameters varied in function of the consistency of the cuttings from each species, but showed a positive correlation between the increase of the rooting and the increase of the ascorbate in the reduced form.Key words: olive tree, peach rootstock, plum rootstock, redox potential, rooting. resuMo a partir de ramos semilenhosos de porta-enxerto de pêssego e ameixa (Prunus cerasifera E.) cv. mrs 2/5, porta-enxerto de ameixa (Prunus cerasifera x P. salicina) x (P. cerasifera x P. persica) cv. ishtarà e oliveira (Olea europaea L.) cv. Frantoio, foram confeccionadas estacas que foram tratadas com 0 mg.L -1 e 2,500 mg.L -1 de ácido indol butírico (iBa) por imersão na base das estacas. após 60 dias de plantio, as cultivares mrs 2/5, ishtarà e Frantoio apresentaram diferença significativa com relação à indução do enraizamento pela aplicação de ácido indol butírico (27.5, 47.5 e 41.25 %, respectivamente), promovendo um maior número de raízes por estaca, sem no entanto aumentar significativamente o comprimento das mesmas. nas estacas enraizadas das três cultivares, os níveis de ascorbato variaram em decorrência da consistência da estaca de cada espécie, todavia demostram uma correlação positiva entre o aumento do enraizamento e o aumento dos níveis de ascorbato na forma reduzida. palavras-chave: enraizamento, oliveira, potencial redox, porta-enxerto de ameixa, porta-enxerto de pêssego.

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Ascorbate and glutathione: guardians of the cell cycle, partners in crime?

Cited by 252 publications

References 91 publications

Buthionine sulfoximine (BSO)-mediated improvement in cultured embryo quality in vitro entails changes in ascorbate metabolism, meristem development and embryo maturation

Buthionine sulfoximine (BSO)-mediated improvement in cultured embryo quality in vitro entails changes in ascorbate metabolism, meristem development and embryo maturation

A role for redox factors in shaping root architecture under Phosphorus deficiency

Extraction and determination of ascorbate and dehydroascorbate from apoplastic fluid of stem of rooted and non-rooted cuttings in relation to the rhizogenesis

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