Ascending Endophytic Migration of Locally Isolated Diazotroph, Enterobacter sp. Strain USML2 in Rice

Tharek, Munirah; Dzulaikha, K.; Salwani, S.; Amir, H. G.; Najimudin, Nazalan

doi:10.3923/biotech.2011.521.527

Cited by 5 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, even the assemblage of fungal communities in plant tissues may be a compromise driven by both functional requirements for plant growth (Torres-Cortes et al, 2018) and local environmental conditions, e.g., the temperature (Campisano et al, 2017); the former may weight out the latter because we found that the fungal similarity within tissues was much higher than that between tissues on a relatively large geographical scale (Figure 3). Within a host, some studies have demonstrated endophytic bacterial migration through tissues (Chi et al, 2005;Tharek et al, 2011). For example, Martí et al (1999) demonstrated that Agrobacterium tumefaciens was recovered from both roots and crowns 3 months after inoculation of rose plant stems.…”

Section: Discussionmentioning

confidence: 99%

Tissue-Specific and Geographical Variation in Endophytic Fungi of Ageratina adenophora and Fungal Associations With the Environment

et al. 2019

View full text Add to dashboard Cite

To understand the distribution of the cultivable fungal community in plant tissues and the associations of these fungi with their surrounding environments during the geographical expansion of an invasive plant, Ageratina adenophora, we isolated the cultivable fungi from 72 plant tissues, 12 soils, and 12 air samples collected from six areas in Yunnan Province, China. A total of 4066 isolates were investigated, including 1641 endophytic fungi, 233 withered leaf fungi, 1255 fungi from air, and 937 fungi from soil. These fungi were divided into 458 and 201 operational taxonomic units (OTUs) with unique and 97% ITS gene sequence identity, respectively. Phylogenetic analysis showed that the fungi belonged to four phyla, including Ascomycota (94.20%), Basidiomycota (2.71%), Mortierellomycota (3.03%), and Mucoromycota (0.07%). The dominant genera of cultivable endophytic fungi were Colletotrichum (34.61%), Diaporthe (17.24%), Allophoma (8.03%), and Fusarium (4.44%). Colletotrichum and Diaporthe were primarily isolated from mature leaves, Allophoma from stems, and Fusarium from roots, indicating that the enrichment of endophytic fungi is tissue-specific and fungi rarely grew systemically within A. adenophora. In the surrounding environment, Alternaria (21.46%), Allophoma (19.31%), Xylaria (18.45%), and Didymella (18.03%) were dominant in the withered leaves, Cladosporium (22.86%), Trichoderma (14.27%), and Epicoccum (9.83%) were dominant in the canopy air, and Trichoderma (27.27%) and Mortierella (20.46%) were dominant in the rhizosphere soils. Further analysis revealed that the cultivable endophytic fungi changed across geographic areas and showed a certain degree of variation in different tissues of A. adenophora. The cultivable fungi in mature and withered leaves fluctuated more than those in roots and stems. We also found that some cultivable endophytic fungi might undergo tissue-to-tissue migration and that the stem could be a transport tissue by which airborne fungi infect roots. Finally, we provided evidence that the fungal community within A. adenophora

show abstract

Section: Discussionmentioning

confidence: 99%

Tissue-Specific and Geographical Variation in Endophytic Fungi of Ageratina adenophora and Fungal Associations With the Environment

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The strain E. coli USML2 used in this study was isolated from the inner segments of leaf tissues of surface sterilised oil palm plant ( Elaeis guineensis Jacq.). The isolate, which was initially identified as an Enterobacter species, was shown to be able to infect and establish an endophytic relationship in rice seedlings Oryza sativa variety MR220 ( Tharek et al 2011 ). However, based on more in-depth molecular identification analysis via polymerase chain reaction (PCR) and amplification of full length 16S rRNA gene fragment (~1.4 kb), the isolate was re-identified as a plant-associated E. coli USML2 ( Tharek et al 2017 ).…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, study by Méric et al (2013) which have isolated E. coli strains from interior plant tissues have not reported any information on the effect of its endophytic presence on growth of its host plant. However, earlier findings by Tharek et al (2011) have successfully isolated E. coli USML2 from inner leaf tissues of surface sterilised healthy oil palm ( Elaeis guineensis Jacq.). The isolate was initially identified as an Enterobacter species and it was shown to be able to infect and establish an endophytic relationship with rice seedlings Oryza sativa (MR220).…”

Section: Introductionmentioning

confidence: 98%

Plant Growth Promoting Potentials of Beneficial Endophytic Escherichia coli USML2 in Association with Rice Seedlings

Tharek

Khairuddin

Najimudin

et al. 2021

TLSR

View full text Add to dashboard Cite

An endophytic Escherichia coli USML2 originally isolated from the inner part of an oil palm (Elaeis guineensis Jacq.) leaf tissue was inoculated to rice seedlings to investigate its ability in colonising plant inner tissues and promoting growth. Infection of E. coli USML2 was initiated by colonisation on the root surface, invasion of the interior root system followed by endophytic spreading. Inoculation of E. coli USML2 in the rice rhizosphere zone resulted in a significant increase in leaf numbers (33.3%), chlorophyll content (33.3%), shoot height (34.8%) and plant dry weight (90.4%) of 42 days old rice seedlings as compared to the control. These findings also demonstrated the ability of E. coli USML2 to spread endophytically which serves as a beneficial strategy for the bacterium to colonise the host plant and gain protection against adverse soil conditions. The genome of E. coli USML2 had also revealed predicted genes essential for endophytic bacterial colonisation and plant growth promotion which further proven potentials of E. coli USML2 as Plant Growth Promoting Endophyte (PGPE).

show abstract

Site and Organ-Specific Culture-Dependent Endophytic Diversity of Crocus sativus L. (Saffron) in Kashmir Himalaya, India

2021

View full text Add to dashboard Cite

Ascending Endophytic Migration of Locally Isolated Diazotroph, Enterobacter sp. Strain USML2 in Rice

Cited by 5 publications

References 16 publications

Tissue-Specific and Geographical Variation in Endophytic Fungi of Ageratina adenophora and Fungal Associations With the Environment

Tissue-Specific and Geographical Variation in Endophytic Fungi of Ageratina adenophora and Fungal Associations With the Environment

Plant Growth Promoting Potentials of Beneficial Endophytic Escherichia coli USML2 in Association with Rice Seedlings

Site and Organ-Specific Culture-Dependent Endophytic Diversity of Crocus sativus L. (Saffron) in Kashmir Himalaya, India

Contact Info

Product

Resources

About