“…This procedure was modeled after the original mitochondrial isolation procedure for mammalian tissues (Schneider and Hogeboom, 1950;Hogeboom et al, 1948). However, other media utilizing isoosmotic mannitol with sucrose in a fashion similar to media used by some investigators for the isolation and/or assay of mitochondria from helninths (Papa et al, 1970;Murfitt et al, 1976;Kohler, 1977;Kohler and Bachmann, 1980;Rodrick et al, 1982) blue crab gill (Chen and Lehninger, 1973) and mammalian tissues (Johnson and Lardy, 1967;Greenawalt, 1974) have been used with some molluscan tissues (Holwerda and de Zwaan, 1979;Vorhaben et al, 1980;de Zwaan et al, 1981;Zaba, 1983) Mitochondria prepared in buffer A had P/0 ratios which were character istic for the particular substrate (glutamate, proline, succinate, malate) and showed a high degree of respiratory control with most of these sub strates (Table 2, Figure 4). Of the substrates tested, only pyruvate failed to generate state 3 respiration (Figure 4).…”