Ascaris suum: Development of intestinal immunity to infective second-stage larvae in swine

Urban, Joseph F.; Alizadeh, Hassan; Romanowski, Robert D.

doi:10.1016/0014-4894(88)90051-3

Cited by 81 publications

(58 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Perhaps ATRA primed or stimulated mast cells to be more responsive to parasite-derived products. Unlike the case for most inbred mouse strains maintained in closed facilities, there are appreciable numbers of mucosal mast cells in uninfected pigs maintained in confined pens without exposure to helminth infection that could account for ATRA-induced mast cell markers (50). In addition, intestinal mucosal mast cells increased following exposure to A. suum and released histamine after exposure to parasite-derived antigens via antibody-dependent cross-linking of surface receptors (3).…”

Section: Vol 77 2009mentioning

confidence: 99%

Localized Th1-, Th2-, T Regulatory Cell-, and Inflammation-Associated Hepatic and Pulmonary Immune Responses inAscaris suum-Infected Swine Are Increased by Retinoic Acid

et al. 2009

View full text Add to dashboard Cite

Pigs infected with Ascaris suum or controls were given 100 g (low-dose) or 1,000 g (high-dose) all-trans retinoic acid (ATRA)/kg body weight in corn oil or corn oil alone per os on days after inoculation (DAI) ؊1, ؉1, and ؉3 with infective eggs. Treatment with ATRA increased interleukin 4 (IL4) and IL12p70 in plasma of infected pigs at 7 DAI and augmented bronchoalveolar lavage (BAL) eosinophilia observed at 7 and 14 DAI. To explore potential molecular mechanisms underlying these observations, a quantitative real-time reverse transcription (RT)-PCR array was used to examine mRNA expression in tissue. Ascaris-infected pigs had increased levels of liver mRNA for T-helper-2 (Th2)-associated cytokines, mast cell markers, and T regulatory (Treg) cells, while infected pigs given ATRA had higher IL4, IL13, CCL11, CCL26, CCL17, CCL22, and TPSB1 expression. Gene expression for Th1-associated markers (IFNG, IL12B, and TBX21), the CXCR3 ligand (CXCL9), IL1B, and the putative Treg marker TNFRSF18 was also increased. Expression of IL4, IL13, IL1B, IL6, CCL11, and CCL26 was increased in the lungs of infected pigs treated with ATRA. To determine a putative cellular source of eosinophil chemoattractants, alveolar macrophages were treated with IL4 and/or ATRA in vitro. IL4 induced CCL11, CCL17, CCL22, and CCL26 mRNA, and ATRA increased the basal and IL4-stimulated expression of CCL17 and CCL22. Thus, ATRA augments a diverse Th1-, Th2-, Treg-, and inflammation-associated response in swine infected with A. suum, and the increased BAL eosinophilia may be related to enhanced induction of eosinophil chemokine activity by alveolar macrophages.

show abstract

Section: Vol 77 2009mentioning

confidence: 99%

Localized Th1-, Th2-, T Regulatory Cell-, and Inflammation-Associated Hepatic and Pulmonary Immune Responses inAscaris suum-Infected Swine Are Increased by Retinoic Acid

et al. 2009

View full text Add to dashboard Cite

show abstract

“…In order to characterize the molecular basis of helminth modulation of allergic disease and to avoid the pathology associated with larval migration, we evaluated the activity of A. suum-derived products in a murine model of allergic inflammation. Pseudocoelomic fluid (PCF) was selected because it is a metabolically active fluid that is easily obtained from the body cavity of adult worms, and it contains a number of parasite antigens that induce antibody responses in several different mammalian species, including mice (15,30). The main protein constituent of PCF is ABA-1, which represents the major IgEbinding component in patients with ascariasis (14,25).…”

mentioning

confidence: 99%

Ascaris suum-Derived Products Suppress Mucosal Allergic Inflammation in an Interleukin-10-Independent Manner via Interference with Dendritic Cell Function

et al. 2006

View full text Add to dashboard Cite

show abstract

“…The decrease in numbers of liver white spots, lung larvae, intestinal larvae and worms of an intermediary size with time all lend support to the existence of a pre-hepatic barrier (Urban et al 1988). This has been shown to develop after continuous exposure to A. suum and may be dose dependent.…”

Section: Discussionmentioning

confidence: 75%

Ascaris suuminfections in pigs born and raised on contaminated paddocks

Mejer¹,

Roepstorff²

2006

Parasitology

View full text Add to dashboard Cite

The transmission of Ascaris suum was studied in outdoor reared pigs. From May to June 2001, 6 farrowing paddocks were naturally contaminated with A. suum using experimentally infected seeder pigs. Early July, 1 sow farrowed on each paddock. One piglet per litter was slaughtered every second week starting at week 3 post-partum (p.p.) for registration of liver white spots and recovery of A. suum from the lungs and the small intestine. The last pigs were slaughtered at week 19 p.p. Faeces was examined for parasite eggs and blood was analysed for A. suum-specific antibodies. Weaning took place at week 7 p.p. by removing the sow. Paddock infection levels were estimated by regular examination of soil samples and in late June and late November using parasite naïve tracer pigs. Paddock contamination was high but eggs developed slowly resulting in a low initial transmission to the experimental pigs. By week 5 p.p. transmission had increased and the numbers of infective eggs in the soil increased during the study. The results indicate a continuous uptake of infective eggs, but visceral larval migration was reduced with time, probably due to the development of a pre-hepatic barrier. Nevertheless, a rather large population of adult worms remained in the pigs throughout the study, and it may primarily have been eggs ingested in the early infection phase that gave rise to the patent infections. It is suggested that neonatal exposure may result in increased persistence and size of adult worm burden and that the higher ‘life-time worm burden’ may be of significant economic importance.

show abstract

Ascaris suum: Development of intestinal immunity to infective second-stage larvae in swine

Cited by 81 publications

References 12 publications

Localized Th1-, Th2-, T Regulatory Cell-, and Inflammation-Associated Hepatic and Pulmonary Immune Responses inAscaris suum-Infected Swine Are Increased by Retinoic Acid

Localized Th1-, Th2-, T Regulatory Cell-, and Inflammation-Associated Hepatic and Pulmonary Immune Responses inAscaris suum-Infected Swine Are Increased by Retinoic Acid

Ascaris suum-Derived Products Suppress Mucosal Allergic Inflammation in an Interleukin-10-Independent Manner via Interference with Dendritic Cell Function

Ascaris suuminfections in pigs born and raised on contaminated paddocks

Contact Info

Product

Resources

About