Asbestos fibers mediate transformation of monkey cells by exogenous plasmid DNA.

Appel, Jan; Fasy, Thomas M.; Kohtz, D. Stave; Kohtz, J D; Johnson, Edward M.

doi:10.1073/pnas.85.20.7670

Cited by 74 publications

(32 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are some data that lend support to this hypothesis: (1) the increase in mesothelioma incidence occurred after millions of people, including adults, were injected with SV40-contaminated vaccines; (2) tissue culture experiments indicated that asbestos facilitated transformation of mouse cells by plasmid DNA and by SV40 (Appel et al, 1988;Dubes, 1993); and (3) mesotheliomeas developed in hamsters injected with SV40 (Cicala et al, 1993).…”

Section: Sv40 and Mesotheliomamentioning

confidence: 99%

Simian virus 40, poliovaccines and human tumors: a review of recent developments

1997

View full text Add to dashboard Cite

Recently, wild-type SV40 and/or DNA sequences indistinguishable from SV40 have been detected in speci®c types of human tumors: ependymoma and choroid plexus tumors, mesothelioma, osteosarcoma and sarcoma. The same tumor types will develop in hamsters after injection with SV40. These ®ndings are interesting in themselves for they could shed light on the pathogenesis of these tumors. These ®ndings also have public health implications. SV40 was found to have contaminated the poliovaccines and the adenovaccines from 1955 until 1963, therefore resulting in the inadvertent injection of millions of people with this tumor virus. Moreover, our society pays a high cost for asbestos causality, a carcinogen associated with the development of mesothelioma. In addition to asbestos, the potential impact of ®nding another possible cause for mesothelioma (i.e., SV40), as well as the possible pathogenic role of the contaminated poliovaccines, has generated considerable public interest and concern. To discuss these recent ®ndings, the NIH (National Institutes of Health) and the FDA (Food and Drug Administration), organized an International Conference at the NIH, Bethesda, MD, January 27 ± 28, 1997. The association of SV40 with human mesothelioma was also discussed in a special session at the IV International Mesothelioma Conference that was held at the University of Pennsylvania, Philadelphia, PA, May 13 ± 16, 1997. The purpose of this review is to summarize data, from the discovery of the contaminated poliovaccines, to the most recent ®ndings presented at the meetings in Bethesda and Philadelphia, to discuss technical and other problems associated with this research, and the potential for using these ®ndings to develop new diagnostic and therapeutic approaches for SV40-associated malignancies.

show abstract

Section: Sv40 and Mesotheliomamentioning

confidence: 99%

Simian virus 40, poliovaccines and human tumors: a review of recent developments

1997

View full text Add to dashboard Cite

show abstract

“…It has been generally accepted that like other asbestos types, chrysotile fibers are capable of inducing human malignant mesothelioma [25][26][27][28] . This conclusion has been obtained from various sources including molecular biological studies [29][30][31][32][33] , animal experiments 2,15,[34][35][36][37][38] , epidemiological studies [39][40][41][42][43][44] , case reports [45][46][47][48] , and asbestos tissue burden studies 6,20) . The present study on asbestos tissue burden further supports the evidence that chrysotile fibers were capable of inducing human malignant mesothelioma, since a) chrysotile was the most common asbestos type seen in the mesothelial tissues which is the original site of the induction of mesothelioma and b) chrysotile was exclusively seen in both the lung and the mesothelial tissues in 15/64 (23.3%) cases, in the lung tissue alone in10/43 (23.3%) and in the mesothelial tissues alone in 30/44 (68.2%) cases.…”

Section: Commentsmentioning

confidence: 99%

Asbestos Tissue Burden Study on Human Malignant Mesothelioma.

Suzuki

Yuen

2001

INDUSTRIAL HEALTH

View full text Add to dashboard Cite

Asbestos fibers in the lung and mesothelial tissues (mesotheliomatous tissue and hyaline plaque) taken from 151 human malignant mesothelioma cases were identified and characterized by high resolution analytical electron microscopy. Asbestos fibers were present in almost all of the lung tissue as well as in the mesothelial tissue. The most common asbestos types seen in the lung were an admixture of chrysotile with amphiboles followed by amphiboles alone and chrysotile alone. The majority of asbestos types seen in the mesothelial tissues were chrysotile alone, followed by chrysotile plus amphibole and amphibole alone. A disproportion of asbestos types between the lung and mesothelial tissues was frequently observed. The most common pattern of the disproportion was chrysotile plus amphibole(s) in the lung and chrysotile only in the mesothelial tissues, followed by amphibole(s) in the lung and chrysotile only in the mesothelial tissues. Such a disproportion was considered to have been caused by chrysotile fiber's strong capacity to translocate from the lung to mesothelial tissues. The number of asbestos fibers in the lung was 456.4 × × × × × 10 6 fibers/dry gram in maximum, 0.08 × × × × × 10 6 fibers/dry gram in minimum and 105 × × × × × 10 6 fibers/dry gram on average; in the mesothelial tissues it was 240.0 × × × × × 10 6 fibers/dry gram in maximum, 0.03 × × × × × 10 6 fibers/dry gram in minimum and 49.84 × × × × × 10 6 fibers/dry gram on average. These numbers were greater than those seen in the general population. The majority of asbestos fibers detected in the lung and mesothelial tissues were shorter than 5 µm in length. Asbestos fibers fit to Stanton's hypothetical dimensions (≥8.0 µm in length and ≤0.25 µm in diameter) were only 4.0%, since the majority of these fibers were shorter (<8 µm) and thinner (<0.25 µm) fibers. We concluded that such short, thin asbestos fibers should not be excluded from those contributing to the induction of human malignant mesothelioma. The present study supports that chrysotile asbestos can induce human malignant mesothelioma, since, in some of the mesothelioma cases, asbestos fibers detected in both the lung and mesothelial tissues, or lung tissue alone or mesothelial tissues alone were exclusively chrysotile fibers.

show abstract

“…Immortalized cells are usually not able to induce tumours but may become oncogenic if they are transfected with an oncogene, or if they are treated with a carcinogen such as asbestos. A synergistic effect between asbestos and SV40 was suggested by the fact that asbestos facilitates transformation of cells in culture by SV40 [19]. In tumours developing in SV40 transgenic mice and in human cells in culture, transient expression of t-antigen seems to be sufficient to induce transformation.…”

Section: Discussionmentioning

confidence: 99%

Detection of SV40 like viral DNA and viral antigens in malignant pleural mesothelioma

Ramael¹,

Nagels²,

Heylen³

et al. 1999

Eur Respir J

View full text Add to dashboard Cite

This study investigated the presence of simian vacuolating virus 40 (SV40) deoxyribonucleic acid (DNA) in malignant pleural mesothelioma, non-neoplastic mesothelium and pleural carcinoma metastasis and correlated these data with immunohistochemistry for SV40 viral antigens.The novel Primed In Situ (PRINS) method was applied to detect the presence of SV40 DNA in situ in tissue sections of malignant mesothelioma (n=25), non-neoplastic mesothelium (n=30) and pleural carcinoma metastasis (n=30). Immunohistochemistry with an SV40-specific antibody was applied for detection of the SV40 viral antigen in the same material.SV40 DNA and expression of one of the viral proteins (small t-antigen) was found iñ 60% of the investigated mesothelioma cases in contrast to non-neoplastic mesothelium and carcinoma metastasis that were negative for both SV40 DNA and SV40 viral antigens.These results suggest that simian vacuolating virus 40 deoxyribonucleic acid may be biologically active as there was also immunoreactivity for simian vacuolating virus 40 viral antigen in those cases positive for simian vacuolating virus 40 deoxyribonucleic acid with the primed in situ reaction. Simian vacuolating virus 40 viral deoxyribonucleic acid and antigens may be potential markers for neoplastic mesothelium that may prove useful in the rather difficult histopathological differential diagnosis between malignant mesothelioma and reactive mesothelium or pleural carcinoma. Eur Respir J 1999; 14: 1381±1386.

show abstract

Asbestos fibers mediate transformation of monkey cells by exogenous plasmid DNA.

Cited by 74 publications

References 40 publications

Simian virus 40, poliovaccines and human tumors: a review of recent developments

Simian virus 40, poliovaccines and human tumors: a review of recent developments

Asbestos Tissue Burden Study on Human Malignant Mesothelioma.

Detection of SV40 like viral DNA and viral antigens in malignant pleural mesothelioma

Contact Info

Product

Resources

About