As You Like It, Part 2: A Critique and Historical Review of the Electron Microscopy Literature

Ghadially, F. N.

doi:10.1080/019131299281789

Cited by 10 publications

(5 citation statements)

References 37 publications

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“…Even though decidualization occurs in normal physiological circumstances, the modified collagen fibrils, of mouse decidua have similarities with some pathologic collagen fibrils such as amianthoid fibrils found in cartilage, ''spiraled'' collagen having peripheral subfibrils in the skin of cattle, and in sheep with dermatosparaxis, in dermal eruptions in Pseudoxanthoma elasticum, and in amyloid kidney (Ghadially, 1982(Ghadially, , 1999. The thickest collagen fibrils (amianthoid fibrils) are found in cartilage, and are thought to be related to regressive or degenerative change (Ghadially, 1982).…”

Section: Discussionmentioning

confidence: 99%

Arrangement and fine structure of collagen fibrils in the decidualized mouse endometrium

Carbone

Pinto

Abrahamsohn

et al. 2006

Microscopy Res & Technique

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The adaptations of the mouse uterus to pregnancy include extensive modifications of the cells and extracellular matrix of the endometrial connective tissue that surround the embryos. Around each implanted embryo this tissue redifferentiates into a transient structure called decidua, which is formed by polygonal cells joined by intercellular junctions. In the mouse, thick collagen fibrils with irregular profile appear in decidualized areas of the endometrium but not in the nondecidualized stroma and interimplantation sites. The fine organization of these thick fibrils has not yet been established. This work was addressed to understand the arrangement and fine structure of collagen fibrils of the decidua of pregnant mice during the periimplantation stage. Major modifications occurred in collagen fibrils that surrounded decidual cells: (1) the fibrils, which were arranged in parallel bundles in nonpregnant animals, became organized as baskets around decidual cells; (2) very thick collagen fibrils with very irregular profiles appeared around decidual cells. Analysis of replicas and serial sections suggests that the thick collagen fibrils form by the lateral aggregation of thinner fibrils to a central fibril resulting in very irregular profile observed in cross sections of thick fibrils. The sum of modifications of the collagen fibrils seem to represent an adaptation of the endometrium to better support the decidual cells while they hold the embryos during the beginning of their development. The deposition of thick collagen fibrils in the decidua may contribute to form a barrier that impedes leukocyte migration within the decidua, preventing immunological rejection of genetically dissimilar embryonic tissues.

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Section: Discussionmentioning

confidence: 99%

Arrangement and fine structure of collagen fibrils in the decidualized mouse endometrium

Carbone

Pinto

Abrahamsohn

et al. 2006

Microscopy Res & Technique

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“…Die Nukleation von Mineral wird durch Zell- und Gewebefragmente stark begünstigt. Dystrophe Kalzifizierung ist besonders gut an frischen Amputationstellen zu beobachten, deren nekrotisches Gewebe reichlich Nuklei für Kalzifizierung bietet 80 83 84 . Neuerdings wird vermutet, dass Zellstress, sobald er Mitochondrien in ihrer Stabilität beeinflusst und so zur Bildung reaktiver Sauerstoffspezies (ROS) und oxidativem Stress führt, also im Endeffekt jeder chronische Zellstress, vorübergehend mikroskopisch kleine Kalzifizierung hervorruft, die rasch wachsen, wenn sie nicht abtransportiert werden 85 .…”

Section: Biomineralisationunclassified

Biologische Mineralisation vs. Pathologische Kalzifizierung – die Rolle des Mineral-Chaperons Fetuin-A

2022

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ZusammenfassungDie Mineralien Kalzium und Phosphat sind für den Zellstoffwechsel aller lebenden Organismen unverzichtbar. Beide Ionen kommen in biologischen Flüssigkeiten normalerweise in millimolaren Konzentrationen vor. Dies führt zu einem Löslichkeits- und Transportproblem, da Kalziumphosphate in Wasser kaum löslich sind und leicht aus übersättigten Lösungen ausfallen. Mineral-Chaperone stabilisieren Mineral als Kolloid und ermöglichen so den Transport und die Clearance nominell übersättigter Mineral-Lösungen. Am Beispiel des Plasmaproteins Fetuin-A erklären wir die Rolle systemischer Mineral-Chaperone, insbesondere bei gestörtem Mineralstoffwechsel. Wir beschreiben den Stoffwechsel kolloidaler Protein-Mineralkomplexen, die in Anlehnung an Lipoprotein-Partikel Calciprotein-Partikel, kurz CPP genannt werden. Wir behandeln die Rolle von CPP bei der physiologischen Knochenbildung und der pathologischen Kalzifizierung. Wir nennen wesentliche Regulatoren von Ossifizierung und Kalzifizierung auf Ebene der Gene, Proteine und Metaboliten. Zuletzt erörtern wir mögliche Therapien von Kalzifizierung anhand eines hierarchischen Modells von mineralinduziertem Stress.

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“…Here they occur ( 1 ) near the surface of muscle cells, displacing the external lamina, ( 2 ) in widened spaces between intracellular junctions, and ( 3 ) within cytoplasmic vacuoles, which are no doubt phagosomes. Spherical microparticles have been seen in ( 1 ) the normal heart of humans, chickens, mice, rats, cats, and sheep, and ( 2 ) the human heart in various pathological states ( valvular disease, congenital heart disease, hypertrophy, degeneration and brosis ).…”

Section: In T H E He a R Tmentioning

confidence: 99%

“…The exact nature of this acquired lysosomal defect is not clear, but one may suggest that ( 1 ) pH in lysosomes may be too high for acid hydrolases to operate but ideal for calcium deposition, and/ or ( 2 ) there may be an overwhelming of the lysosomal apparatus by bacteria and other substrates, and in time the necrotic debris in the lysosome calci es ( as does lipid -rich necrotic debris elsewhere ). As mentioned earlier calci cation occurs not only in association with matrical lipidic debris be it large ( cell fragments ) or small ( so -called``matrix vesicles´´or`s pherical microparticles´´) but also in and around collagen brils.…”

Section: Intra Lys Os Oma L Ca Lcifica Tionmentioning

confidence: 99%

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As You Like It, Part 3: A Critique and Historical Review of Calcification as Seen with the Electron Microscope

Ghadially

2001

Ultrastructural Pathology

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As You Like It, Part 3, is a continuation of the lectures given by the author (Ultra Path VIII and Ultra Path IX). It is a critical historical review of topics of interest to electron microscopists, attempting to show what went wrong and perhaps also why. The topics chosen this time demonstrate the prominent role electron microscopy has played in elucidating the diverse ways in which calcification can occur. The classic concept of dystropic and metastatic calcification is now inadequate to explain all observed phenomena. The electron microscope shows that calcification occurs in many different intracellular and extracellular sites and that each has its own morphology and etiology. Thus, a new classification based on ultrastructural morphology is born, but few seem to be aware of it. The author examines the ubiquitous but not too well-known phenomenon of cell remodeling, which often, but not always, leads to calcification. Topics are presented under the following headings: (1) matrical lipidic debris, (2) spherical microparticles, (3) matrix vesicles, (4) intramitochondrial calcification, (5) intralysosomal calcification, (6) calcification of collagen, (7) calcification of elastic fibers, and (8) calcification of secretory products.

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As You Like It, Part 2: A Critique and Historical Review of the Electron Microscopy Literature

Cited by 10 publications

References 37 publications

Arrangement and fine structure of collagen fibrils in the decidualized mouse endometrium

Arrangement and fine structure of collagen fibrils in the decidualized mouse endometrium

Biologische Mineralisation vs. Pathologische Kalzifizierung – die Rolle des Mineral-Chaperons Fetuin-A

As You Like It, Part 3: A Critique and Historical Review of Calcification as Seen with the Electron Microscope

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