Aryl Polyenes, a Highly Abundant Class of Bacterial Natural Products, Are Functionally Related to Antioxidative Carotenoids

Schöner, Tim A.; Gassel, Sören; Osawa, Ayako; Tobias, Nicholas J.; Okuno, Yukari; Sakakibara, Yui; Shindo, Kazutoshi; Sandmann, Gerhard; Bode, Helge B.

doi:10.1002/cbic.201500474

Cited by 154 publications

(144 citation statements)

References 39 publications

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“…While the potential to form these linear precursors was observed, genes for squalene and lycopene cyclization were not identified. An aryl polyene biosynthetic gene cluster was identified in FLA‐8, which may produce a carotenoid‐like pigment possibly involved in photoprotection and scavenging of reactive oxygen species (Schöner et al ., ). An additional cluster in FLA‐8 contains genes for phytoene/squalene synthase, phytoene desaturase, lycopene cyclase domain‐containing protein and beta‐carotene hydroxylase (Supporting Information Fig.…”

Section: Resultsmentioning

confidence: 99%

Metabolic relationships of uncultured bacteria associated with the microalgae Gambierdiscus

Rambo

Dombrowski

Constant

et al. 2019

Environmental Microbiology

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Microbial communities inhabit algae cell surfaces and produce a variety of compounds that can impact the fitness of the host. These interactions have been studied via culturing, single-gene diversity and metagenomic read survey methods that are limited by culturing biases and fragmented genetic characterizations. Higher-resolution frameworks are needed to resolve the physiological interactions within these algalbacterial communities. Here, we infer the encoded metabolic capabilities of four uncultured bacterial genomes (reconstructed using metagenomic assembly and binning) associated with the marine dinoflagellates Gambierdiscus carolinianus and G. caribaeus. Phylogenetic analyses revealed that two of the genomes belong to the commonly algae-associated families Rhodobacteraceae and Flavobacteriaceae. The other two genomes belong to the Phycisphaeraceae and include the first algae-associated representative within the uncultured SM1A02 group. Analyses of all four genomes suggest these bacteria are facultative aerobes, with some capable of metabolizing phytoplanktonic organosulfur compounds including dimethylsulfoniopropionate and sulfated polysaccharides. These communities may biosynthesize compounds beneficial to both the algal host and other bacteria, including iron chelators, B vitamins, methionine, lycopene, squalene and polyketides. These findings have implications for marine carbon and nutrient cycling and provide a greater depth of understanding regarding the genetic potential for complex physiological interactions between microalgae and their associated bacteria.

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Section: Resultsmentioning

confidence: 99%

Metabolic relationships of uncultured bacteria associated with the microalgae Gambierdiscus

Rambo

Dombrowski

Constant

et al. 2019

Environmental Microbiology

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“…kururiensis genomes. Aryl‐polyenes (APEs) are pigments similar to carotenoids and have been associated to bacterial protection against light‐induced damage and reactive oxygen species (ROS) (Cimermancic et al., ; Schoener et al., ). In all three genomes, the clusters comprise 40 kb and harbor approximately 45 genes, from which 35% of the genes show similarity with APE cluster from V .…”

Section: Resultsmentioning

confidence: 99%

Comparative genomics of Paraburkholderia kururiensis and its potential in bioremediation, biofertilization, and biocontrol of plant pathogens

et al. 2019

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Burkholderia harbors versatile Gram‐negative species and is β‐Proteobacteria. Recently, it was proposed to split the genus in two main branches: one of animal and plant pathogens and another, Paraburkholderia, harboring environmental and plant‐beneficial species. Currently, Paraburkholderia comprises more than 70 species with ability to occupy very diverse environmental niches. Herein, we sequenced and analyzed the genome of Paraburkholderia kururiensis type strain KP23T, and compared to P. kururiensis M130, isolated in Brazil, and P. kururiensis susbp. thiooxydans, from Korea. This study focused on the gene content of the three genomes with special emphasis on their potential of plant‐association, biocontrol, and bioremediation. The comparative analyses revealed several genes related to plant benefits, including biosynthesis of IAA, ACC deaminase, multiple efflux pumps, dioxygenases, and degradation of aromatic compounds. Importantly, a range of genes for protein secretion systems (type III, IV, V, and VI) were characterized, potentially involved in P. kururiensis well documented ability to establish endophytic association with plants. These findings shed light onto bacteria‐plant interaction mechanisms at molecular level, adding novel information that supports their potential application in bioremediation, biofertilization, and biocontrol of plant pathogens. P. kururiensis emerges as a promising model to investigate adaptation mechanisms in different ecological niches.

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“…According to AntiSMASH, the genome possesseses five secondary metabolite gene clusters with 14 putative gene clusters. Detected secondary metabolites include ectoin (osmolites protective substance) (Bernard et al 1983), resorcinal, arylopolyene (protects from reactive oxygen species) (Schöner Tim et al 2016), and terpene (ecological role), phosphonate (global phosphorus cycle) (Yu et al 2013) that supports the bacteria for surviving in harsh environment (Supplementary file 2).…”

Section: Secondary Metabolites Analysismentioning

confidence: 99%

Peer Review #1 of "Adaptation of metal and antibiotic resistant traits in novel β-Proteobacterium Achromobacter xylosoxidans BHW-15 (v0.2)"

2019

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Chromosomal coexistence of metal and antibiotic resistance genes in bacteria offers a new perspective to the bacterial resistance proliferation in contaminated environment. In this study, an arsenotrophic bacterium Achromobacter xylosoxidans BHW-15, isolated from Arsenic (As) contaminated tubewell water in Bogra district of Bangladesh, was analyzed using high throughput Ion Torrent PGM complete genome sequencing scheme to reveal its adaptive potentiality. The assembled draft genome of A. xylosoxidans BHW-15 was 6.3 Mbp containing 5782 functional genes, 1845 pseudo genes, and 3 incomplete phage signature regions. Comparative genome study suggested the bacterium to be a novel strain of A. xylosoxidans showing significant dissimilarity with other relevant strains in metal resistance gene islands. Thirty five metal resistance genes along with arseniteoxidizing aioSXBA, arsenate reducing arsRCDAB, and mercury resistance merRTPADE operonic gene cluster and twenty broad range antibiotic resistance genes including βlactams, aminoglycosides, and multiple multidrug resistance (MDR) efflux gene complex with a tripartite system OM-IM-MFP were found co-existed within the genome. Genomic synteny analysis with reported arsenotrophic bacteria revealed the characteristic genetic organization of ars and mer operonic genes, rarely described in β-Proteobacteria. A transposon Tn21 and mobile element protein genes were also detected to the end of mer (mercury) operonic genes, possibly a carrier for the gene transposition. In vitro antibiotic susceptibility assay showed a broad range of resistance against antibiotics belonging to βlactams, aminoglycosides, cephalosporins (1 st , 2 nd and 3 rd generations), monobactams and even macrolides, some of the resistome determinants were predicted during in silico analysis. KEGG functional orthology analysis revealed the potential of the bacterium to utilize multiple carbon sources including one carbon pool by folate, innate defense mechanism against multiple stress conditions, motility, a proper developed cell signaling and processing unit and secondary metabolism-combination of all exhibiting a robust

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Aryl Polyenes, a Highly Abundant Class of Bacterial Natural Products, Are Functionally Related to Antioxidative Carotenoids

Cited by 154 publications

References 39 publications

Metabolic relationships of uncultured bacteria associated with the microalgae Gambierdiscus

Metabolic relationships of uncultured bacteria associated with the microalgae Gambierdiscus

Comparative genomics of Paraburkholderia kururiensis and its potential in bioremediation, biofertilization, and biocontrol of plant pathogens

Peer Review #1 of "Adaptation of metal and antibiotic resistant traits in novel β-Proteobacterium Achromobacter xylosoxidans BHW-15 (v0.2)"

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