Artificially synthesized helper/killer-hybrid epitope long peptide (H/K-HELP): Preparation and immunological analysis of vaccine efficacy

Masuko, Kazutaka; Wakita, Daiko; Togashi, Yuji; Kita, Takafumi; Kitamura, Hiroyuki; Nishimura, Takashi

doi:10.1016/j.imlet.2014.11.016

Cited by 12 publications

(9 citation statements)

References 33 publications

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“…Future efforts will be undertaken to improve the immunity-inducing ability of MGlu-HPG liposomes by introduction of adjuvant functions such as cationic lipid, CpG-ODN, cytokine gene. In addition, higher antigenic long peptides have been developed for the induction of both CTL and Th cells [28]. The use of such long peptides is also expected to enhance the therapeutic effects of peptide-based vaccines.…”

Section: Resultsmentioning

confidence: 99%

Improvement of Peptide-Based Tumor Immunotherapy Using pH-Sensitive Fusogenic Polymer-Modified Liposomes

et al. 2016

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Abstract:To establish peptide vaccine-based cancer immunotherapy, we investigated the improvement of antigenic peptides by encapsulation with pH-sensitive fusogenic polymer-modified liposomes for induction of antigen-specific immunity. The liposomes were prepared by modification of egg yolk phosphatidylcholine and L-dioleoyl phosphatidylethanolamine with 3-methyl-glutarylated hyperbranched poly(glycidol) (MGlu-HPG) and were loaded with antigenic peptides derived from ovalbumin (OVA) OVA-I (SIINFEKL), and OVA-II (PSISQAVHAAHAEINEAP β A), which bind, respectively, to major histocompatibility complex (MHC) class I and class II molecules on dendritic cell (DCs). The peptide-loaded liposomes were taken up efficiently by DCs. The peptides were delivered into their cytosol. Administration of OVA-I-loaded MGlu-HPG-modified liposomes to mice bearing OVA-expressing E.G7-OVA tumors induced the activation of OVA-specific CTLs much more efficiently than the administration of free OVA-I peptide did. Mice strongly rejected E.G7-OVA cells after immunization with OVA-I peptide-loaded MGlu-HPG liposomes, although mice treated with free OVA-I peptide only slightly rejected the cells. Furthermore, efficient suppression of tumor volume was observed when tumor-bearing mice were immunized with OVA-I-peptide-loaded liposomes. Immunization with OVA-II-loaded MGlu-HPG-modified liposomes exhibited much lower tumor-suppressive effects. Results indicate that MGlu-HPG liposomes might be useful for improvement of CTL-inducing peptides for efficient cancer immunotherapy.

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Section: Resultsmentioning

confidence: 99%

Improvement of Peptide-Based Tumor Immunotherapy Using pH-Sensitive Fusogenic Polymer-Modified Liposomes

et al. 2016

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“…In this regard, CTL/Th-hybrid epitope long peptides, containing both CTL-and Th-epitopes, were reported to successfully induce both antigen-specific CTL and Th1. 15,22 Unlike T-cell epitope short peptides, T-cell epitope long peptides are taken up mainly by APCs and can avoid immunologic tolerance induced by direct contact with T-cells and B-cells. 13 In addition, T-cell epitope long peptides facilitate DC cross-presentation 14 and lead to more potent antigen-specific CTL induction.…”

Section: Discussionmentioning

confidence: 99%

“…LLO 91‐99 (GYKDGNEYI) and LLO 215‐226 (SQLIAKFGTAFK) were from MBL and SCRUM (Tokyo, Japan), respectively. In terms of T‐cell epitope long peptides, as shown in Figure 1, OVA epitope long peptide was synthesized by conjugating CTL (OVA 257‐264 ) and Th (OVA 323‐339 ) epitope peptides with glycine‐linker 22 in the Fmoc solid‐phase peptide synthesis method (SCRUM; Figure 1a). In the same way, we synthesized LLO T‐cell epitope long peptide by conjugating CTL (LLO 91‐99 ) and Th (LLO 215‐226 ) epitope peptides with glycine‐linker (SCRUM; Figure 1b).…”

Section: Methodsmentioning

confidence: 99%

Development of a novel T cell‐oriented vaccine using CTL/Th‐hybrid epitope long peptide and biodegradable microparticles, against an intracellular bacterium

Tanaka

Enomoto

Uehara

et al. 2020

Microbiology and Immunology

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Antigen-specific CD8+ T-lymphocytes (cytotoxic T-lymphocytes: CTL), as well as CD4+ T-lymphocytes (helper T-lymphocytes: Th), simultaneously play an important role in the elimination of intracellular bacteria such as Mycobacterium tuberculosis and Listeria monocytogenes. Administration of T-cell epitope short peptide needs large numbers of peptides for effective vaccination due to its easily degradable nature in vivo. In this respect, biocompatible and biodegradable microparticles combined with CTL/Thhybrid epitope long peptide (long peptide) have been used to diminish the degradation of loaded peptide. The aim of this study is to develop a novel T cell-oriented vaccine against intracellular bacteria that is composed of long peptide and poly (lactic-co-glycolic acid) (PLGA) microparticles. Mouse bone marrow-derived dendritic cells (BMDCs) were loaded with L. monocytogenes listeriolysin O (LLO)-derived or ovalbumin (OVA)-derived long peptide/PLGA or other comparative antigens. The antigen-loaded BMDCs were injected subcutaneously into the flank of mice twice, and then, the spleens were collected and lymphocyte proliferation and interferon-γ production were evaluated. The median diameter of the PLGA spheres was 1.38 μm. Both LLO-and OVA-long peptide/PLGA showed significantly more robust CTL and Th proliferations with higher interferon-γ production than the long peptide alone or CTL and Th short peptides/PLGA vaccination. Furthermore, the LLO-long peptide/PLGA vaccination showed a significantly lower bacterial burden in spleens compared with the long peptide

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“…already reported the use of such artificial SLP made of peptides containing class I and class II epitopes connected via an artificial linker and documented enhanced CD4 and CD8 T cell responses in vitro 9 and in vivo in a mouse model. 10 In both publications, they chose a five glycine linker to design their SLP which was better than a five alanine or five proline linker.…”

Section: Discussionmentioning

confidence: 99%

“…An alternative strategy for designing SLP vaccines relies on a careful selection of well-defined CD8 and CD4 epitopes, for which a wide repertoire exists and/or elicits strong immune responses. 9,10 Selection of both CD4 and CD8 epitopes offers a wide range of opportunities: separation of naturally overlapping epitopes, linking epitopes that are otherwise far apart on the natural antigen or creation of chimeric CONTACT François Lang francois.lang@univ-nantes.fr UMR-Inserm U1232, Institut de Recherche Thérapeutique de l'Université de Nantes, 8 Quai Moncousu BP 70721, Nantes Cedex 1 44007, France Color versions of one or more of the figures in the article can be found online at www.tandfonline.com/koni.…”

Section: Introductionmentioning

confidence: 99%

Cancer vaccines: designing artificial synthetic long peptides to improve presentation of class I and class II T cell epitopes by dendritic cells

et al. 2019

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Artificially synthesized helper/killer-hybrid epitope long peptide (H/K-HELP): Preparation and immunological analysis of vaccine efficacy

Cited by 12 publications

References 33 publications

Improvement of Peptide-Based Tumor Immunotherapy Using pH-Sensitive Fusogenic Polymer-Modified Liposomes

Improvement of Peptide-Based Tumor Immunotherapy Using pH-Sensitive Fusogenic Polymer-Modified Liposomes

Development of a novel T cell‐oriented vaccine using CTL/Th‐hybrid epitope long peptide and biodegradable microparticles, against an intracellular bacterium

Cancer vaccines: designing artificial synthetic long peptides to improve presentation of class I and class II T cell epitopes by dendritic cells

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