Artificial ribonucleases inactivate a wide range of viruses using their ribonuclease, membranolytic, and chaotropic-like activities

Fedorova, Antonina A.; Гончарова, Е. П.; Королева, Л. С.; Burakova, E.; Ryabchikova, E. I.; Bichenkova, Elena V.; Silnikov, Vladimir N.; Vlassov, Valentin V.; Zenkova, Marina A.

doi:10.1016/j.antiviral.2016.07.014

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…Several reports also suggested a mild fibrinolytic activity of iloprost [ 53 , 54 , 55 , 56 ]. Antimicrobial effect was furnished by a cationic amphiphilic drug (CAD) 1,5-bis-(4-tetradecyl-1,4-diazoniabicyclo [2.2.2] octan-1-yl) pentane tetrabromide, which has RNase activity in contrast to other CADs and therefore has higher antibacterial and antiviral properties [ 33 , 57 , 58 , 59 ]. Both iloprost and CAD were conjugated to the TEVG luminal surface using a poly(vinylpyrrolidone) (PVP) hydrogel.…”

Section: Resultsmentioning

confidence: 99%

Controlled and Synchronised Vascular Regeneration upon the Implantation of Iloprost- and Cationic Amphiphilic Drugs-Conjugated Tissue-Engineered Vascular Grafts into the Ovine Carotid Artery: A Proteomics-Empowered Study

et al. 2022

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Implementation of small-diameter tissue-engineered vascular grafts (TEVGs) into clinical practice is still delayed due to the frequent complications, including thrombosis, aneurysms, neointimal hyperplasia, calcification, atherosclerosis, and infection. Here, we conjugated a vasodilator/platelet inhibitor, iloprost, and an antimicrobial cationic amphiphilic drug, 1,5-bis-(4-tetradecyl-1,4-diazoniabicyclo [2.2.2]octan-1-yl) pentane tetrabromide, to the luminal surface of electrospun poly(ε-caprolactone) (PCL) TEVGs for preventing thrombosis and infection, additionally enveloped such TEVGs into the PCL sheath to preclude aneurysms, and implanted PCLIlo/CAD TEVGs into the ovine carotid artery (n = 12) for 6 months. The primary patency was 50% (6/12 animals). TEVGs were completely replaced with the vascular tissue, free from aneurysms, calcification, atherosclerosis and infection, completely endothelialised, and had clearly distinguishable medial and adventitial layers. Comparative proteomic profiling of TEVGs and contralateral carotid arteries found that TEVGs lacked contractile vascular smooth muscle cell markers, basement membrane components, and proteins mediating antioxidant defense, concurrently showing the protein signatures of upregulated protein synthesis, folding and assembly, enhanced energy metabolism, and macrophage-driven inflammation. Collectively, these results suggested a synchronised replacement of PCL with a newly formed vascular tissue but insufficient compliance of PCLIlo/CAD TEVGs, demanding their testing in the muscular artery position or stimulation of vascular smooth muscle cell specification after the implantation.

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Section: Resultsmentioning

confidence: 99%

Controlled and Synchronised Vascular Regeneration upon the Implantation of Iloprost- and Cationic Amphiphilic Drugs-Conjugated Tissue-Engineered Vascular Grafts into the Ovine Carotid Artery: A Proteomics-Empowered Study

et al. 2022

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“…Poloxamer 188 (Kolliphor 188) was purchased from BASF Schweiz AG (Kaisten, Germany). Cationic surfactants (mono-and dicationic DABCO and quinuclidine) were synthesized at the Arbuzov Institute of Organic and Physical Chemistry of the FRC Kazan Scientific Center of Russian Academy of Sciences (Kazan, Russia) [17][18][19]. Ultra-purified water was obtained from Milli ® Q Plus system (Darmstadt, Germany), home supplied.…”

Section: Methodsmentioning

confidence: 99%

“…The saturated bicyclic framework of these compounds is found in natural physiologically active compounds [17]. In addition, these surfactants display antibacterial [18] and antiviral [18] activity as well as improved solubility properties for hydrophobic dyes and drugs [17,19]. In this work, cationic NEs composed of mono-and dicationic DABCO and quinuclidine surfactants produced and supplied by Arbuzov Institute of Organic and Physical Chemistry of the FRC Kazan Scientific Center of Russian Academy of Sciences (S1-S9) (Figure 1) have been used to exploit their antimicrobial profile in drug delivery systems for ocular administration.…”

Section: Introductionmentioning

confidence: 99%

Mono- and Dicationic DABCO/Quinuclidine Composed Nanomaterials for the Loading of Steroidal Drug: 32 Factorial Design and Physicochemical Characterization

et al. 2021

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Oil-in-water nanoemulsions (NEs) are considered a suitable nanotechnological approach to improve the eye-related bioavailability of lipophilic drugs. The potential of cationic NEs is prominent due to the electrostatic interaction that occurs between the positively charged droplets with the negatively charged mucins present in the tear film. This interaction offers prolonged NEs residence at the ocular surface, increasing the drug absorption. Triamcinolone acetonide (TA) is one of the first pharmacologic strategies applied as an intravitreal injection in the treatment of age-related macular degeneration (AMD). Newly synthesized quaternary derivatives of 1,4-diazabicyclo[2.2.2]octane (DABCO) and quinuclidine surfactants have been screened with the purpose to select the best compound to formulate long-term stable NEs that combine the best physicochemical properties for the loading of TA intended for ocular administration.

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“…Polycationic amphiphiles based on 1,4‒diazabicyclo[2.2.2]octane (DABCO) have a wide spectrum of biological activity: antibacterial, antifungal and antiviral [ 6 , 7 , 8 ]. Structurally, these compounds consist of two DABCO residues with a long lateral hydrophobic substituent connected by a linker group.…”

Section: Introductionmentioning

confidence: 99%

Changes in the Ultrastructure of Staphylococcus aureus Cells Make It Possible to Identify and Analyze the Injuring Effects of Ciprofloxacin, Polycationic Amphiphile and Their Hybrid

Grigor’eva,

Bardasheva,

Ryabova

et al. 2023

Microorganisms

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The purposeful development of synthetic antibacterial compounds requires an understanding of the relationship between effects of compounds and their chemical structure. This knowledge can be obtained by studying changes in bacteria ultrastructure under the action of antibacterial compounds of a certain chemical structure. Our study was aimed at examination of ultrastructural changes in S. aureus cells caused by polycationic amphiphile based on 1,4‒diazabicyclo[2.2.2]octane (DL412), ciprofloxacin and their hybrid (DL5Cip6); the samples were incubated for 15 and 45 min. DL412 first directly interacted with bacterial cell wall, damaging it, then penetrated into the cell and disrupted cytoplasm. Ciprofloxacin penetrated into cell without visually damaging the cell wall, but altered the cell membrane and cytoplasm, and inhibited the division of bacteria. The ultrastructural characteristics of S. aureus cells damaged by the hybrid clearly differed from those under ciprofloxacin or DL412 action. Signs associated with ciprofloxacin predominated in cell damage patterns from the hybrid. We studied the effect of ciprofloxacin, DL412 and their hybrid on S. aureus biofilm morphology using paraffin sections. Clear differences in compound effects on S. aureus biofilm (45 min incubation) were observed. The results obtained allow us to recommend this simple and cheap approach for the initial assessment of antibiofilm properties of synthesized compounds.

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Artificial ribonucleases inactivate a wide range of viruses using their ribonuclease, membranolytic, and chaotropic-like activities

Cited by 5 publications

References 38 publications

Controlled and Synchronised Vascular Regeneration upon the Implantation of Iloprost- and Cationic Amphiphilic Drugs-Conjugated Tissue-Engineered Vascular Grafts into the Ovine Carotid Artery: A Proteomics-Empowered Study

Controlled and Synchronised Vascular Regeneration upon the Implantation of Iloprost- and Cationic Amphiphilic Drugs-Conjugated Tissue-Engineered Vascular Grafts into the Ovine Carotid Artery: A Proteomics-Empowered Study

Mono- and Dicationic DABCO/Quinuclidine Composed Nanomaterials for the Loading of Steroidal Drug: 32 Factorial Design and Physicochemical Characterization

Changes in the Ultrastructure of Staphylococcus aureus Cells Make It Possible to Identify and Analyze the Injuring Effects of Ciprofloxacin, Polycationic Amphiphile and Their Hybrid

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