Artificial receptor layers for detecting chemical and biological agent mimics

Findeisen, Anna; Wackerlig, Judith; Samardzic, Renata; Pitkänen, J.; Anttalainen, Osmo; Dickert, Franz L.; Lieberzeit, Peter A.

doi:10.1016/j.snb.2011.08.025

Cited by 26 publications

(15 citation statements)

References 16 publications

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“…However, the sensor was sensitive to other CW agents and was not selective to DMMP. Selectivity toward DMMP vapors can be improved by using MIP (molecularly imprinted polymers) layers [15]. However, for on-field applications they are not preferred because of slow response and recovery time [15].…”

Section: Introductionmentioning

confidence: 99%

“…Selectivity toward DMMP vapors can be improved by using MIP (molecularly imprinted polymers) layers [15]. However, for on-field applications they are not preferred because of slow response and recovery time [15]. Hence efforts are still continuing toward the enhancement of sensitivity and selectivity toward DMMP detection at room temperature.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Origin and role of elasticity in the enhanced DMMP detection by ZnO/SAW sensor

Raj

Singh

Nimal

et al. 2015

Sensors and Actuators B: Chemical

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Origin and role of elasticity in the enhanced DMMP detection by ZnO/SAW sensor

Raj

Singh

Nimal

et al. 2015

Sensors and Actuators B: Chemical

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“…Quartz crystal microbalances (QCMs) were prepared by screen-printing the desired dual electrode structure with commercial brilliant gold paste (purchased from HERAEUS, Hanau, Germany) on an AT-cut quartz disc with a diameter of 13.8 mm and a thickness of 168 μm (purchased from Great Microtama Industries, Surabaya, Indonesia), following a previously developed procedure [ 25 ]. After printing, devices were heated to 400 °C for 4 h in order to remove organic residues (linseed oil) from the paste and to reveal the metallic gold electrodes.…”

Section: Methodsmentioning

confidence: 99%

Combined Layer/Particle Approaches in Surface Molecular Imprinting of Proteins: Signal Enhancement and Competition

Phan

Sussitz

Ladenhauf

et al. 2018

Sensors

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Herein we report novel approaches to the molecular imprinting of proteins utilizing templates sizing around 10 nm and some 100 nm. The first step comprised synthesizing nanoparticles of molecularly imprinted polymers (MIP) towards bovine serum albumin (BSA) and characterizing them according to size and binding capacity. In a second step, they were utilized as templates. Quartz crystal microbalances (QCM) coated with MIP thin films based on BSA MIP nanoparticles lead to a two-fold increase in sensor responses, compared with the case of directly using the protein as the template. This also established that individual BSA molecules exhibit different “epitopes” for molecular imprinting on their outer surfaces. In light of this knowledge, a possible MIP-based biomimetic assay format was tested by exposing QCM coated with BSA MIP thin films to mixtures of BSA and imprinted and non-imprinted polymer (NIP) nanoparticles. At high protein concentrations (1000 ppm) measurements revealed aggregation behavior, i.e., BSA binding MIP NP onto the MIP surface. This increased sensor responses by more than 30% during proof of concept measurements. At lower a BSA concentration (500 ppm), thin films and particles revealed competitive behavior.

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“…In another study, Tokonami et al [15], effectively developed a label free and selective E. coli identification by surface imprinting the whole cell imprinting of bacteria on the surface of overoxidized polypyrrole, which could detect the bacteria within the range of 10 3 -10 9 CFU/mL. Findeisen et al [16] studied biological agent detection via MIP based artificial receptor layers, and used E. coli as model agent. They used spin coated polymeric film with 300-400 nm thicknesses and achieved a lower detection limit of 0.1 mg/mL E. coli.…”

Section: Introductionmentioning

confidence: 99%