Artificial oocyte activation with calcium ionophore A23187 in intracytoplasmic sperm injection cycles using surgically retrieved spermatozoa

Borges, Edson; Braga, Daniela Paes de Almeida Ferreira; Bonetti, Tatiana Carvalho de Sousa; Iaconelli, Assumpto; Franco, J.G.

doi:10.1016/j.fertnstert.2008.04.046

Cited by 41 publications

(16 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The improved quality of embryos in several reports may be related to optimal oocyte activation, as optimized calcium oscillations can affect embryo development. Calcium oscillations may also participate in long-term embryonic events that go beyond its role as the stimulus for meiotic resumption [ 18 ]. The exact mechanism by which intracellular calcium influences embryonic development is not completely understood; however, it has been postulated that the calcium oscillation pattern may partly act through gene expression regulation [ 19 20 ].…”

Section: Discussionmentioning

confidence: 99%

Artificial oocyte activation in intracytoplasmic sperm injection cycles using testicular sperm in humanin vitrofertilization

Kang

Lee

Park

et al. 2015

Clin Exp Reprod Med

View full text Add to dashboard Cite

ObjectiveArtificial oocyte activation (AOA) is an effective method to avoid total fertilization failure in human in vitro fertilization-embryo transfer (IVF-ET) cycles. AOA performed using a calcium ionophore can induce calcium oscillation in oocytes and initiate the fertilization process. We evaluated the usefulness of AOA with a calcium ionophore in cases of total fertilization failure in previous cycles and in cases of severe male factor infertility patients with non-motile spermatozoa after pentoxifylline (PF) treatment.MethodsThe present study describes 29 intracytoplasmic sperm injection (ICSI)-AOA cycles involving male factor infertility at Cheil General Hospital from January 2006 to June 2013. Patients were divided into two groups (control, n=480; AOA, n=29) depending on whether or not AOA using a calcium ionophore (A23187) was performed after testicular sperm extraction-ICSI (TESE-ICSI). The AOA group was further split into subgroups according to sperm motility after PF treatment: i.e., motile sperm-injected (n=12) and non-motile sperm-injected (n=17) groups (total n=29 cycles).ResultsThe good embryo rate (52.3% vs. 66.9%), pregnancy rate (20.7% vs. 52.1%), and delivery rate (10.3% vs. 40.8%) were lower in the PF/AOA group than in the control group. When evaluating the effects of restoration of sperm motility after PF treatment on clinical outcomes there was no difference in fertilization rate (66.6% vs. 64.7% in non-motile and motile sperm, respectively), pregnancy rate (17.6% vs. 33.3%), or delivery rate (5.9% vs. 16.7%) between the two groups.ConclusionWe suggest that oocyte activation is a useful method to ensure fertilization in TESE-ICSI cycles regardless of restoration of sperm motility after PF treatment. AOA may be useful in selected patients who have a low fertilization rate or total fertilization failure.

show abstract

Section: Discussionmentioning

confidence: 99%

Artificial oocyte activation in intracytoplasmic sperm injection cycles using testicular sperm in humanin vitrofertilization

Kang

Lee

Park

et al. 2015

Clin Exp Reprod Med

View full text Add to dashboard Cite

show abstract

“…Total fertilization failure is basically a failure of oocyte activation (Ebner et al, 2004;Sousa and Tesarik, 1994). Several reports have shown improved fertilization in patients with a history of complete or partial fertilization failure after applying different AOA methods (Borges et al, 2009), such as electrical (Baltaci et al, 2010;Mansour et al, 2009), mechanical (Tesarik et al, 2002) and chemical activation (Nakagawa et al, 2001;Tejera et al, 2008;Kashir et al, 2010;Ebner et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

A preliminary report of successful cleavage after calcium ionophore activation at ICSI in cases with previous arrest at the pronuclear stage

Darwish¹,

Magdi²

2015

Reproductive BioMedicine Online

View full text Add to dashboard Cite

Artificial oocyte activation (AOA) has been previously suggested as a means to overcome the problem of total fertilization failure, which affects about 1-3% of the intracytoplasmic sperm injection (ICSI) cycles. A preliminary study on the application of chemical AOA was conducted using A23187 Ca(2+) ionophore to improve embryonic development in four women with a history of complete fertilization arrest and inability to transit to cleavage stage during previous ICSI trials. Data indicated that activated oocytes resulted in better fertilization, embryonic development and clinical pregnancy in one of the four couples. Therefore, ICSI combined with AOA using Ca(2+) ionophore may be useful in selected patients with cleavage failure, and may help the zygotes to reach more advanced developmental stages.

show abstract

“…Modifications to the zona pellucida and release from meiotic arrest ensue. Artificial methods to activate the oocyte have now been developed, that improve the rate of fertilization (especially in cases of low fertility) and can be performed using chemical agents such as strontium chloride (Kumagai, 2006) and calcium ionophores (Murase et al , 2004; Borges et al , 2009) or by mechanical (Dirican et al , 2008) or electrical (Yanagida et al , 1999) stimulation. In the experiments described herein, strontium chloride was used.…”

Section: Discussionmentioning

confidence: 99%

Methodology matters: IVF versus ICSI and embryonic gene expression

Bridges

Jeoung

Heyoung

et al. 2011

Reproductive BioMedicine Online

View full text Add to dashboard Cite

The use of assisted reproduction treatment, especially intracytoplasmic sperm injection (ICSI), is now linked to a range of adverse consequences, the aetiology of which remains largely undefined. The objective was to determine differences in gene expression of blastocysts generated by ICSI as well as ICSI with artificial oocyte activation (ICSI-A) versus the less manipulative IVF, providing fundamental genetic information that can be used to aid in the diagnosis or treatment of those adversely affected by assisted reproduction treatment, as well as stimulate research to further refine these techniques. Murine blastocysts were generated by ICSI, ICSI-A and IVF, and processed for a microarray-based analysis of gene expression. Ten blastocysts were pooled for each procedure and three independent replicates generated. The data were then processed to determine differential gene expression and to identify biological pathways affected by the procedures. In blastocysts derived by ICSI versus IVF, the expression of 197 genes differed (P < 0.01). In blastocysts derived by ICSI-A versus IVF and ICSI-A versus ICSI, the expression of 132 and 65 genes differed respectively (P < 0.01). Procedural-induced changes in genes regulating specific biological pathways revealed some consistency to known adverse consequences. Detailed investigation of procedure-specific dysfunction is therefore warranted.

show abstract

Artificial oocyte activation with calcium ionophore A23187 in intracytoplasmic sperm injection cycles using surgically retrieved spermatozoa

Cited by 41 publications

References 54 publications

Artificial oocyte activation in intracytoplasmic sperm injection cycles using testicular sperm in humanin vitrofertilization

Artificial oocyte activation in intracytoplasmic sperm injection cycles using testicular sperm in humanin vitrofertilization

A preliminary report of successful cleavage after calcium ionophore activation at ICSI in cases with previous arrest at the pronuclear stage

Methodology matters: IVF versus ICSI and embryonic gene expression

Contact Info

Product

Resources

About