Artificial Neural Network Study of Whole-Cell Bacterial Bioreporter Response Determined Using Fluorescence Flow Cytometry

Abstract

Genetically engineered bioreporters are an excellent complement to traditional methods of chemical analysis. The application of fluorescence flow cytometry to detection of bioreporter response enables rapid and efficient characterization of bacterial bioreporter population response on a single-cell basis. In the present study, intrapopulation response variability was used to obtain higher analytical sensitivity and precision. We have analyzed flow cytometric data for an arsenic-sensitive bacterial bioreporter using an artificial neural network-based adaptive clustering approach (a single-layer perceptron model). Results for this approach are far superior to other methods that we have applied to this fluorescent bioreporter (e.g., the arsenic detection limit is 0.01 microM, substantially lower than for other detection methods/algorithms). The approach is highly efficient computationally and can be implemented on a real-time basis, thus having potential for future development of high-throughput screening applications.

Biosensors: Classifications, medical applications, and future prospective

“…The VITOTOX ۚ test has been employed for genotoxicity assessment of several drugs during the early phases of drugs development . GenoTox uses the colicin D gene cda (a constituent of the ColD plasmid) and gfp as a report gene . The signal green fluorescent was measured using flow cytometry to improve the overall sensitivity of the fluorescent biosensors.…”

The effect of natural organic ligands on trace metal speciation in San Francisco Bay: Implications for water quality criteria

“…As with, for instance, linear regression, a test statistic (referred to in this case as vigilance) is used to delineate the boundary between similar and dissimilar signatures. Further details concerning calculation of Euclidian distance and use of vigilance can be found in Busam et al (2007).…”

Metrological approaches to drug development. Fast screening using universal biosensors at the stage of drug development

“…Acute toxicity, LM Safe dilution multiplicity, SM Chronic toxicity, LM developed for biological activity testing at all stages of the pharmaceutical technology: from the stage of synthesis and isolation of a substance to production of a new pharmaceutical preparation [26][27][28][29][30]. These biosensors can be used in pharmaceutics for functional screening at the stage of drug design and discovery [31] and for predictive screening at the stage of planning the targeted synthesis and determination of the mechanism of action of the biologically active substance [32], and for toxicity predictions over a wide concentration range without animal experiments [33,34].…”