Artificial linear episome-based protein expression system for protozoon Leishmania tarentolae

Kushnir, Susanna; Cirstea, Ion Cristian; Basiliya, Lyudmyla; Lupilova, Nataliya; Breitling, Reinhard; Alexandrov, Kirill

doi:10.1016/j.molbiopara.2010.12.002

Cited by 22 publications

(17 citation statements)

References 21 publications

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“…These cells are non-pathogenic for mammals (Taylor et al, 2010) and grow readily in Brain Heart Infusion (BHI) (Difco) at 27˚C with hemin added to 10 μg/ml (Simpson et al, 1970). L. tarentolae has been studied extensively as a model trypanosomatid and has been recently developed and commercialized as a useful eukaryotic expression vector (Basile and Peticca, 2009; Breitling et al, 2002; Dortay and Mueller-Roeber, 2010; Fritsche et al, 2007; Klatt and Konthur, 2012; Kovtun et al, 2010; Kushnir et al, 2011; Kushnir et al, 2005). …”

Section: Introductionmentioning

confidence: 99%

U-insertion/deletion RNA editing multiprotein complexes and mitochondrial ribosomes in Leishmania tarentolae are located in antipodal nodes adjacent to the kinetoplast DNA

et al. 2015

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We studied the intramitochondrial localization of several multiprotein complexes involved in U-insertion/deletion RNA editing in trypanosome mitochondria. The editing complexes are located in one or two antipodal nodes adjacent to the kinetoplast DNA (kDNA) disk, which are distinct from but associated with the minicircle catenation nodes. In some cases the proteins are in a bilateral sheet configuration. We also found that mitoribosomes have a nodal configuration. This type of organization is consistent with evidence for protein and RNA interactions of multiple editing complexes to form a ~40S editosome and also an interaction of editosomes with mitochondrial ribosomes.

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Section: Introductionmentioning

confidence: 99%

U-insertion/deletion RNA editing multiprotein complexes and mitochondrial ribosomes in Leishmania tarentolae are located in antipodal nodes adjacent to the kinetoplast DNA

et al. 2015

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“…129 A new type of expression methodology based on linear elements has been developed, where linear episomes with telomere-like structures are transfected into the cells, allowing formation of a culture overexpressing the recombinant protein of interest, without clonal selection of the transfected cells. 130 Proteins produced in L. tarentolae include the recombinant human coagulation factor VII, 131 and the glycosylated soluble amyloid precursor protein a (sAPPa), a cleavage product of the etiologic agent in Alzheimer disease, APP. 132 Another study produced the ORF2 coding for a truncated capsid protein of porcine hepatitis E virus, in order to develop an ELISA.…”

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confidence: 99%

Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules

Legastelois

Buffin

Peubez

et al. 2016

Human Vaccines & Immunotherapeutics

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The increasing demand for recombinant vaccine antigens or immunotherapeutic molecules calls into question the universality of current protein expression systems. Vaccine production can require relatively low amounts of expressed materials, but represents an extremely diverse category consisting of different target antigens with marked structural differences. In contrast, monoclonal antibodies, by definition share key molecular characteristics and require a production system capable of very large outputs, which drives the quest for highly efficient and cost-effective systems. In discussing expression systems, the primary assumption is that a universal production platform for vaccines and immunotherapeutics will unlikely exist. This review provides an overview of the evolution of traditional expression systems, including mammalian cells, yeast and E.coli, but also alternative systems such as other bacteria than E. coli, transgenic animals, insect cells, plants and microalgae, Tetrahymena thermophila, Leishmania tarentolae, filamentous fungi, cell free systems, and the incorporation of non-natural amino acids.

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“…Its flexibility e nables solutions f or constitutive or inducible expression of intracellular or secreted proteins. The target gene constructs can be either stably integrated into the chromosomal multicopy b-tubulin (tub) or singular ornithine decarboxylase (odc) loci [173] or maintained episomally [170]. Inducible expression is accomplished in an engineered L. tarentolae strain co-expressing bacteriophage T7 RNA polymerase and tetracycline repressor.…”

Section: Big Lines To Classes Of Products and Main Marketsmentioning

confidence: 99%

Suspension Culture of Protozoan Organisms

Hartmann¹,

Breitling

2014

Industrial Scale Suspension Culture of Living Cells

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Artificial linear episome-based protein expression system for protozoon Leishmania tarentolae

Cited by 22 publications

References 21 publications

U-insertion/deletion RNA editing multiprotein complexes and mitochondrial ribosomes in Leishmania tarentolae are located in antipodal nodes adjacent to the kinetoplast DNA

U-insertion/deletion RNA editing multiprotein complexes and mitochondrial ribosomes in Leishmania tarentolae are located in antipodal nodes adjacent to the kinetoplast DNA

Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules

Suspension Culture of Protozoan Organisms

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