Artificial interspecific backcrosses between the hybrid of female Crassostrea hongkongensis × male C. gigas and the two parental species

Zhang, Yuehuan; Zhang, Yang; Li, Jun; Xiao, Shijun; Xiang, Zhiming; Wang, Zhaoping; Yan, Xiwu; Zhou, Yu

doi:10.1016/j.aquaculture.2015.07.013

Cited by 23 publications

(7 citation statements)

References 59 publications

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“…The salinity level at their original habitat (the collection site) was 25‰ fluctuating with tide frequently. In previous studies, salinity 25‰ was used as the salinity of acclimation and proved as an appropriate salinity for physiological activities in both oyster species 45 52 53 54 . Salinity 8‰ is almost a extreme salinity for C. gigas 41 55 , which was used as treated salinity level for osmotic stress.…”

Section: Discussionmentioning

confidence: 99%

High throughput sequencing of small RNAs transcriptomes in two Crassostrea oysters identifies microRNAs involved in osmotic stress response

Zhao

Kong

et al. 2016

Sci Rep

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Increasing evidence suggests that microRNAs post-transcriptionally regulate gene expression and are involved in responses to biotic and abiotic stress. However, the role of miRNAs involved in osmotic plasticity remains largely unknown in marine bivalves. In the present study, we performed low salinity challenge with two Crassostrea species (C. gigas and C. hongkongensis), and conducted high-throughput sequencing of four small RNA libraries constructed from the gill tissues. A total of 202 and 87 miRNAs were identified from C. gigas and C. hongkongensis, respectively. Six miRNAs in C. gigas and two in C. hongkongensis were differentially expressed in response to osmotic stress. The expression profiles of these eight miRNAs were validated by qRT-PCR. Based on GO enrichment and KEGG pathway analysis, genes associated with microtubule-based process and cellular component movement were enriched in both species. In addition, five miRNA-mRNA interaction pairs that showed opposite expression patterns were identified in the C. hongkongensis, Differential expression analysis identified the miRNAs that play important regulatory roles in response to low salinity stress, providing insights into molecular mechanisms that are essential for salinity tolerance in marine bivalves.

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Section: Discussionmentioning

confidence: 99%

High throughput sequencing of small RNAs transcriptomes in two Crassostrea oysters identifies microRNAs involved in osmotic stress response

Zhao

Kong

et al. 2016

Sci Rep

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“…There are several recent papers addressing various questions regarding cross‐breeding of Hong Kong oyster with other oysters (Zhang et al . , ; Huo, Wang, Yan & Gaffney ; Zhang, Zhang, Li, Su, Guan, Xiao, Li & Yu ; Zhang, Zhang, Li, Wang, Yan & Yu ,b; Zhang, Zhang, Li, Xiao, Xiang, Wang, Yan & Yu ). However, in spite of its high economic value, there is limited information available about triploidy in C .…”

Section: Introductionmentioning

confidence: 99%

“…Due to its recent identification as a separate species distinct from Jinjiang oyster, C. rivularis (Boudry, Heurtebise & Lapegue 2003;Lam & Morton 2003;Wang, Guo, Zhang & Zhang 2004), there are relatively few studies regarding Hong Kong oyster genetics and breeding. There are several recent papers addressing various questions regarding cross-breeding of Hong Kong oyster with other oysters (Zhang et al 2012(Zhang et al , 2014Huo, Wang, Yan & Gaffney 2013;Zhang, Zhang, Li, Su, Guan, Xiao, Li & Yu 2015c;Zhang, Zhang, Li, Wang, Yan & Yu 2015a,b;Zhang, Zhang, Li, Xiao, Xiang, Wang, Yan & Yu 2016). However, in spite of its high economic value, there is limited information available about triploidy in C. hongkongensis and its genetic advantages with respect to aquaculture.…”

Section: Introductionmentioning

confidence: 99%

A comparative study of the survival, growth and gonad development of the diploid and triploid Hong Kong oyster, Crassostrea hongkongensis (Lam & Morton 2003)

Zhang

Qin

et al. 2016

Aquac Res

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The Hong Kong oyster, Crassostrea hongkongensis is an economically important and widely cultivated oyster species in southern China. Aiming to improve the aquaculture potentials of C. hongkongensis, a triploid organism was generated by inhibiting the second polar body formation in the fertilized eggs, using 0.5 mg L−1 cytochalasin B. Triploids exhibited significantly higher growth and were larger than the diploids throughout the life cycle. Much of the triploid growth advantage can be attributed to resource reallocation to structural development due to gonadal sterility. Most of the triploid oysters exhibited polymorphic sterility with atrophic gonads and abnormal gametogenesis. Only few ‘dysfunctional’ sperms were produced by the triploid males, thus ruling out reproductive and genetic contamination of natural oyster populations from triploid cultivations. This advantage in addition to the superior growth of triploids makes it a potent alternative in Hong Kong oyster aquaculture industry. Triploid survival was mostly found to be similar to that of diploids except for day 360, when a significant triploid advantage in terms of survival was noted. Furthermore, we found that growth and survival of Hong Kong oyster is significantly higher at the Zhuhai site in comparison to the Beihai site. Our results indicate a strong environmental effect in Hong Kong oyster cultivation and points to the fact that Zhuhai site was more suitable for oyster farming than the Beihai site. Overall, this study provides valuable information to the aquaculture industry of southern China.

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“…Furthermore, there is currently limited evidence for the application of heterosis for traits related to disease resistance in molluscs, albeit higher general survival has been shown in some cases [53–55]. While substantial knowledge gaps remain, there may be potential to harness the beneficial effects of heterosis within the context of family-based breeding programmes, for example via the breeding and maintenance of specialist lines for cross-breeding, as commonly occurs in the pig breeding sector [56].…”

Section: Breeding Programme Designmentioning

confidence: 99%

Potential of genomic technologies to improve disease resistance in molluscan aquaculture

Potts

Gutiérrez

Peñaloza

et al. 2021

Phil. Trans. R. Soc. B

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Molluscan aquaculture is a major contributor to global seafood production, but is hampered by infectious disease outbreaks that can cause serious economic losses. Selective breeding has been widely used to improve disease resistance in major agricultural and aquaculture species, and has clear potential in molluscs, albeit its commercial application remains at a formative stage. Advances in genomic technologies, especially the development of cost-efficient genomic selection, have the potential to accelerate genetic improvement. However, tailored approaches are required owing to the distinctive reproductive and life cycle characteristics of molluscan species. Transgenesis and genome editing, in particular CRISPR/Cas systems, have been successfully trialled in molluscs and may further understanding and improvement of genetic resistance to disease through targeted changes to the host genome. Whole-organism genome editing is achievable on a much greater scale compared to other farmed species, making genome-wide CRISPR screening approaches plausible. This review discusses the current state and future potential of selective breeding, genomic tools and genome editing approaches to understand and improve host resistance to infectious disease in molluscs. This article is part of the Theo Murphy meeting issue ‘Molluscan genomics: broad insights and future directions for a neglected phylum’.

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Artificial interspecific backcrosses between the hybrid of female Crassostrea hongkongensis × male C. gigas and the two parental species

Cited by 23 publications

References 59 publications

High throughput sequencing of small RNAs transcriptomes in two Crassostrea oysters identifies microRNAs involved in osmotic stress response

High throughput sequencing of small RNAs transcriptomes in two Crassostrea oysters identifies microRNAs involved in osmotic stress response

A comparative study of the survival, growth and gonad development of the diploid and triploid Hong Kong oyster, Crassostrea hongkongensis (Lam & Morton 2003)

Potential of genomic technologies to improve disease resistance in molluscan aquaculture

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