Aims: The study aimed to investigate the adoption and perception of artificial intelligence (AI) chatbots, particularly those powered by large language models (LLMs), among pathologists worldwide. It explored the extent of their engagement with these technologies, identifying potential impacts on their professional practices. Methods: A cross-sectional survey was conducted, gathering data from pathologists on their usage and views concerning AI chatbots powered by LLMs. The survey, distributed globally via various digital platforms, included both quantitative and qualitative questions. Statistical analyses were performed to delineate patterns in the adoption and perspectives on these AI tools among the respondents. Results: Of 215 respondents, 100 (46.5%) reported using LLMs, particularly ChatGPT, for professional purposes, predominantly for information retrieval, proofreading, and academic writing, highlighting a significant time-saving benefit. The adoption varied across demographics, with younger, male pathologists showing higher usage rates. While the technology was mainly utilized for drafting academic materials and programming tasks, users expressed concerns about information accuracy, privacy, and the need for regulatory approval. Despite recognizing occasional inaccuracies, respondents saw potential in advanced AI features, particularly in image analysis and speech-to-text functions. Conclusions: The survey underscored pathologists' cautious yet growing interest in leveraging LLMs to enhance information accessibility, efficiency, and medical education. While the potential benefits are recognized, significant apprehensions about the reliability, ethics, and security associated with these AI tools underscore the need for comprehensive regulation and standardized practices to ensure their responsible use in the medical field.