Artificial Insemination Trial of Frozen-Thawed Boar Spermatozoa with Thawing Solution Containing Seminal Plasma

Okazaki, Tetsuji; Akiyoshi, Teiichi; Kan, M.T; Teshima, Hisanori; Shimada, M.

doi:10.5938/youton.48.164

Cited by 3 publications

(4 citation statements)

References 11 publications

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“…In AI examination using frozen‐thawed sperm prepared according to the above protocol, a 78% farrowing rate and a mean litter size of 10.1 piglets was obtained (Okazaki et al . 2011b). However, such high success rates with AI have been recorded in research centers, and similar results obtained using related techniques in commercial settings have been reported (Eriksson et al .…”

Section: Introductionmentioning

confidence: 99%

Addition of oxytocin to semen extender improves both sperm transport to the oviduct and conception rates in pigs following AI

Okazaki¹,

Ikoma²,

Tinen³

et al. 2013

Animal Science Journal

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Oxytocin (OXT) contained in boar semen is known to produce uterine contraction; therefore, we hypothesized that the co-injection of OXT with sperm would improve artificial insemination (AI) using liquid or frozen-thawed boar sperm. We initially examined whether OXT added to semen extender improved sperm transport to the oviduct. Although the addition of OXT did not affect the fresh or frozen-thawed sperm motility or acrosomal integrity, it significantly increased the number of sperm in the oviduct at 6 h after AI injection with OXT, as compared with the control (P < 0.05). Moreover, some sperm were observed in the sperm reservoir of the isthmus in the OXT treatment group, whereas few sperm were observed in the control. When OXT was added to the semen extender immediately prior to AI, the conception rates were significantly higher in both fresh semen and frozen-thawed semen than in the control group (P < 0.05: liquid, 87.5% vs. 70.5%; frozen-thawed, 89.8% vs. 75.0%). From these results, we concluded that the addition of OXT to the semen extender assisted in sperm transportation from the uterus to the oviduct, which resulted in improved reproductive performance.

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Section: Introductionmentioning

confidence: 99%

Addition of oxytocin to semen extender improves both sperm transport to the oviduct and conception rates in pigs following AI

Okazaki¹,

Ikoma²,

Tinen³

et al. 2013

Animal Science Journal

Self Cite

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“…To clear the reasons why the ovulation process is not synchronized and takes a long time to induce the ovulation process in pig compared with those in rodent model, we examined the kinetic changes of gene expression and acti- Table 3. The reproductive performance of artificial insemination using fresh semen and frozen-thawed semen (from Okazaki et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…From these reports, we estimated that unstabilization of cell membrane during thawing process would allow the uptake of Ca 2+ in sperm cytoplasm to induce the abnormal capacitation and the loss of acrosome cap. To clear this hypothesis, we investigated the level of Ca 2+ in sperm after thawing and the additional effects of Ca 2+ chelator, EGTA to thawing solution on [Ca 2+ ] i level and post-thawed sperm functions (Okazaki et al 2011).…”

Section: -4 Ca 2+ Uptake In Sperm During Thawing Process Damages Spmentioning

confidence: 99%

Development of Artificial Insemination Technique in Pig Production Based on the Evidence of Physiology and Molecular Biology in Reproductive Organs

Shimada¹,

Yamashita²,

Okazaki³

2016

AGri-Biosci. Monogr. (AGBM)

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pean countries, more than 70% of pig farms carry out artificial insemination using liquid storage semen, and this rate reaches more than 98% in the Netherlands (Feitsma 2009). However, the diffusion of this technique has been still less than half in other countries including Japan. The improvements of both insemination technique and diluent extender of boar semen Abstract Reproductive technology in the animal production field not only improves the reproductive performance of swine but also reduces the production costs. However, the diffusion of this technique has been limited in most countries including Japan due to the reproductive characteristics of pig, such as multiple ovulations in female and large volume of semen in male. In females, the key mechanisms of oocyte maturation in pig are not different as compared with that of the rodent model. However, the timing and regulation of gene expression and enzyme activity are characterized in pig, which results in the longer duration of oocyte maturation and ovulation. In semen, seminal plasma has dual roles in sperm quality and successful implantation. The contaminating of bacteria in semen increases endotoxin activity and reduces the sperm quality. On the other hand, the seminal plasma contains factors to regulate immune activity to reduce the number of leukocytes to make an optimal condition for implantation and to maintain the pregnancy. Based on basic scientific information, we developed novel techniques for oocyte in vitro maturation, freezing and thawing sperm and artificial insemination. These technologies will be expanded to commercial farms to increase the production-efficiency all over the world.

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“…The optimal concentration of seminal plasma added to the thawing solution was 10% (v/v). Moreover, in the AI test, the conception rate and the mean litter size of sows ( n = 81) inseminated with frozen‐thawed sperm in 10% (v/v) seminal plasma were 80.2% and 10.1 piglets, values that are comparable to reproductive performance in fresh semen (conception rate; 73.9%, litter size; 10.3 piglets) (Okazaki et al . 2011).…”

Section: Positive Effects Of Seminal Plasma During the Thawing Processmentioning

confidence: 94%

New strategies of boar sperm cryopreservation: Development of novel freezing and thawing methods with a focus on the roles of seminal plasma

Okazaki¹,

Shimada

2012

Animal Science Journal

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Cryopreservation of boar spermatozoa offers an effective means of long‐term storage of important genetic material. Many researchers have investigated how to improve reproductive performance by artificial insemination (AI) using cryopreserved boar spermatozoa. Recently, we and other groups reported that high conception rates (70–80%) can be achieved by AI with frozen‐thawed boar spermatozoa using a modified temperature program during freezing, or a novel cryopreservation extender to improve sperm quality (including sperm survivability, motility, membrane status and fertilization ability) after thawing, or a novel sperm infusion method, deep intra uterine insemination. However, these techniques have not yet been used for commercial pig production. The variation in sperm freezability among boars or among ejaculations in an identical boar is one of the main reasons for this problem. In our previous study, it was revealed that some components of seminal plasma have a negative effect on the freezability of boar sperm. One of these factors is bacteria‐released endotoxin (lipopolysaccharide: LPS). LPS binds to Toll‐like receptor‐4 (TLR‐4) expressed on the sperm surface, resulting in induction of apoptosis. On the other hand, seminal plasma suppresses cryo‐capacitation induced by thawing stress. On the basis of these findings, we designed a novel protocol of AI using frozen‐thawed boar sperm.

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Artificial Insemination Trial of Frozen-Thawed Boar Spermatozoa with Thawing Solution Containing Seminal Plasma

Cited by 3 publications

References 11 publications

Addition of oxytocin to semen extender improves both sperm transport to the oviduct and conception rates in pigs following AI

Addition of oxytocin to semen extender improves both sperm transport to the oviduct and conception rates in pigs following AI

Development of Artificial Insemination Technique in Pig Production Based on the Evidence of Physiology and Molecular Biology in Reproductive Organs

New strategies of boar sperm cryopreservation: Development of novel freezing and thawing methods with a focus on the roles of seminal plasma

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