Artificial Insemination in Horses—More than a Century of Practice and Research

Aurich, Jörg

doi:10.1016/j.jevs.2012.06.011

Cited by 40 publications

(30 citation statements)

References 24 publications

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“…One of the underlying reasons for this lies in the genetic selection of stallions, which is mainly based on productive performance, sports and morphological characteristics, leaving the reproductive characteristics aside (Clulow, Maxwell, Evans, & Morris, ). Artificial insemination (AI) in horses is currently a common practice worldwide; however, due to the inefficiency of semen cryopreservation in this species, the use of fresh or cooled semen is preferred over frozen semen (Aurich, ). However, this practice presents several disadvantages associated mainly with the low lifespan of non‐frozen semen and the impossibility to transport these samples between countries or over long distances making the use of frozen semen highly necessary for the equine industry (Aurich, ).…”

Section: Introductionmentioning

confidence: 99%

“…Artificial insemination (AI) in horses is currently a common practice worldwide; however, due to the inefficiency of semen cryopreservation in this species, the use of fresh or cooled semen is preferred over frozen semen (Aurich, ). However, this practice presents several disadvantages associated mainly with the low lifespan of non‐frozen semen and the impossibility to transport these samples between countries or over long distances making the use of frozen semen highly necessary for the equine industry (Aurich, ).…”

Section: Introductionmentioning

confidence: 99%

“…semen and the impossibility to transport these samples between countries or over long distances making the use of frozen semen highly necessary for the equine industry (Aurich, 2012).…”

mentioning

confidence: 99%

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Cryopreservation of stallion semen: Effect of adding antioxidants to the freezing medium on sperm physiology

Contreras

Treulén

Arias

et al. 2020

Reprod Domestic Animals

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Cryopreservation of stallion semen has not reached the level of efficiency and positive results described in other species. This is mainly due to the greater sensitivity of stallion sperm to the freezing process, showing higher rates of oxidative stress and plasma membrane damage, which trigger the activation of several cell damage pathways that ultimately culminate in DNA fragmentation and cell death. Therefore, finding molecules that improve the efficiency of this technique in stallion by preventing oxidative stress and cell damage is required. Thus, the aim of the present study was to evaluate the effect of adding three antioxidants (MnTBAP, NAC and FeTPPS) to the freezing medium on the quality and functional parameters of stallion sperm. Semen samples from three stallions frozen with the antioxidants were evaluated in two conditions: (a) adding the antioxidants before freezing, and (b) before and after freezing. Plasma membrane integrity, mitochondrial membrane potential, lipid peroxidation, intracellular ROS levels, membrane lipid disorder, DNA damage, sperm motility and binding to the zona pellucida were assessed. The results showed that MnTBAP was the antioxidant treatment that best controlled the oxidative stress process and post‐thaw cell damage, showing higher plasma membrane integrity, mitochondrial membrane potential, sperm motility, number of spermatozoa bound to the zona pellucida of bovine oocytes and lower lipid disorder. Additionally, it was determined that a second post‐thaw application of antioxidants is detrimental since induced higher cell damage and lower sperm motility, without showing any beneficial effect on the spermatozoa.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cryopreservation of stallion semen: Effect of adding antioxidants to the freezing medium on sperm physiology

Contreras

Treulén

Arias

et al. 2020

Reprod Domestic Animals

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“…In the horse, the accepted number of viable spermatozoa for insemination is more individual dependent than in other species [3]. Studies have consistently shown CP sperm lack in motility, viability and intact membranes when compared to that of fresh semen [5]. Hence, in the horse, the development of successful freezing procedures will involve more than the identification or application of novel CPO's and additives [3].…”

Section: Discussionmentioning

confidence: 99%

Cryoprotectants & Cryopreservation of Equine Semen: A Review of Industry Cryoprotectants and the Effects of Cryopreservation on Equine Semen Membranes

Prien¹

2016

JDVAR

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“…The use of frozen semen in horse breeding is constantly increasing (Aurich, ), but post‐thaw semen quality and fertilization rates are still lower than with fresh and cooled‐stored semen (Loomis & Graham, ; Pena et al., ). Moreover, there is a large variability in semen viability and pregnancy rates among stallions and breeds (Alvarenga et al., ; Hoffmann, Oldenhof, Morandini, Rohn, & Sieme, ; Sieme, Harrison, & Petrunkina, ; Vidament, ).…”

Section: Introductionmentioning

confidence: 99%

Influence of partial or total replacement of glycerol by alternative cryoprotectants in Ghent freezing extender on post‐thaw sperm quality in stallions

Oliveira

Budik

Aurich

2017

Reprod Domestic Animals

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Although glycerol is the cryoprotectant most commonly used in stallions, it has also a considerable toxicity for equine sperm. It was the aim of this study to analyse the quality of frozen-thawed stallion semen after complete or partial replacement of glycerol in the freezing extender by alternative cryoprotectants. We hypothesized that partial or total replacement of glycerol by cryoprotectants occurring in cold-resistant frog, insect or plant species results in similar or better semen quality after freezing-thawing. As basic medium, the commercial Ghent basic extender was used and either supplemented with glucose and urea, trehalose and proline, or trehalose and betaine. Based on a series of preliminary experiments, semen was frozen in either commercial Ghent cryopreservation extender (Ghent control), Ghent glucose-urea extender or a Ghent combined extender (glucose-urea, trehalose-betaine and trehalose-proline; volume ratio of 2:1:2) in a computer-controlled rate freezer. After freezing-thawing, semen was analysed for motility, membrane integrity, phosphatidylserine translocation, mitochondrial membrane potential and chromatin condensation. No differences between Ghent control and Ghent glucose-urea extender were seen, while all endpoints except DNA integrity were negatively affected in Ghent combined extender (e.g., progressive motility: Ghent 49.2 ± 3.7, Ghent glucose-urea 46.5 ± 4.6, Ghent combined 24.4 ± 2.8%; p < .001). In conclusion, glycerol concentration in a commercial freezing extender for equine spermatozoa can be successfully reduced when urea as an additive cryoprotectant is added and the glucose concentration is elevated. However, total glycerol replacement with urea, betaine, proline and trehalose was less successful.

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Artificial Insemination in Horses—More than a Century of Practice and Research

Cited by 40 publications

References 24 publications

Cryopreservation of stallion semen: Effect of adding antioxidants to the freezing medium on sperm physiology

Cryopreservation of stallion semen: Effect of adding antioxidants to the freezing medium on sperm physiology

Cryoprotectants & Cryopreservation of Equine Semen: A Review of Industry Cryoprotectants and the Effects of Cryopreservation on Equine Semen Membranes

Influence of partial or total replacement of glycerol by alternative cryoprotectants in Ghent freezing extender on post‐thaw sperm quality in stallions

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